Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument

Marquardt, Drew; Frontzek, Matthias D.; Zhao, Yu; Chakoumakos, Bryan C.; Katsaras, John

doi:10.1107/S1600576718001243

Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument

Journal Article · Mon Feb 05 23:00:00 EST 2018 · Journal of Applied Crystallography (Online)

DOI:https://doi.org/10.1107/S1600576718001243· OSTI ID:1435280

Marquardt, Drew ^[1]; Frontzek, Matthias D. ^[2]; Zhao, Yu ^[3]; Chakoumakos, Bryan C. ^[2]; Katsaras, John ^[2]

Univ. of Windsor, ON (Canada)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Pennsylvania State Univ., University Park, PA (United States)

Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H₂O/D₂O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1435280

Journal Information:: Journal of Applied Crystallography (Online), Journal Name: Journal of Applied Crystallography (Online) Journal Issue: 2 Vol. 51; ISSN 1600-5767; ISSN JACGAR

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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A suite-level review of the neutron powder diffraction instruments at Oak Ridge National Laboratory Calder, S.; An, K.; Boehler, R. Review of Scientific Instruments, Vol. 89, Issue 9 https://doi.org/10.1063/1.5033906	journal	September 2018
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