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Title: Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds

Journal Article · · Journal of Biomaterials Applications
 [1];  [2];  [3];  [2];  [4];  [5]; ORCiD logo [5];  [6];  [7];  [3]; ORCiD logo [8]
  1. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine. Dept. of Ophthalmology and Visual Sciences
  2. Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering
  3. Univ. of Edinburgh, Scotland (United Kingdom). Inst. for Bioengineering
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  6. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Molecular Microbiology and Immunology. Dept. of Surgery
  7. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine
  8. Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering; Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Surgery
+ Show Author Affiliations

Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This paper examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus (S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Finally, colony forming cell assay and in vivo live imaging results strongly suggested the inhibition of S. aureus growth.

Research Organization:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Manufacturing Office; USDOE Office of Science (SC)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1435225
Journal Information:
Journal of Biomaterials Applications, Vol. 33, Issue 1; ISSN 0885-3282
Country of Publication:
United States
Language:
English

References (40)

Global trends in emerging infectious diseases journal February 2008
Quorum Sensing and the Social Evolution of Bacterial Virulence journal February 2009
Burns, biofilm and a new appraisal of burn wound sepsis journal February 2010
Scalable production of microbially mediated zinc sulfide nanoparticles and application to functional thin films journal October 2014
Synthesis and mechanical behavior of nanostructured materials via cryomilling journal January 2006
Mechanism of Immunologic Suppression in Burn Injury journal January 1990
Size-Dependent Bacterial Growth Inhibition and Mechanism of Antibacterial Activity of Zinc Oxide Nanoparticles journal April 2011
Mechanisms of Phagocytosis in Macrophages journal April 1999
Scalable economic extracellular synthesis of CdS nanostructured particles by a non-pathogenic thermophile journal September 2013
Effect of Macrolide Consumption on Erythromycin Resistance in Streptococcus pyogenes in Finland in 1997-2001 journal May 2004
An in vitro biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates journal March 2011
Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms journal April 2002
Calculations of the structural properties of cubic zinc sulfide journal April 1990
Fluorescent reporters for Staphylococcus aureus journal June 2009
Comparative Toxicity of Nanoparticulate ZnO, Bulk ZnO, and ZnCl 2 to a Freshwater Microalga (Pseudokirchneriella subcapitata): The Importance of Particle Solubility journal December 2007
Bacteria and wound healing journal January 2004
One-pot synthesis of monodispersed ZnS nanospheres with high antibacterial activity journal January 2010
Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms by Pseudomonas aeruginosa and Staphylococcus aureus journal April 2009
Silver against Pseudomonas aeruginosa biofilms journal August 2007
An Organoselenium Compound Inhibits Staphylococcus aureus Biofilms on Hemodialysis Catheters In Vivo journal November 2011
The role of Bacteria in burn Mortality journal August 1968
Antimicrobial tolerance and the significance of persister cells in recalcitrant chronic wound biofilms: Antimicrobial tolerance in wound biofilms journal January 2011
The Different Behavior of Rutile and Anatase Nanoparticles in Forming Oxy Radicals Upon Illumination with Visible Light: An EPR Study journal November 2011
Investigation of ZnS Nanoparticle Antibacterial Effect journal October 2014
Abrogation of the inflammatory response in LPS-stimulated RAW 264.7 murine macrophages by Zn- and Cu-doped bioactive sol-gel glasses journal August 2009
Next Science Wound Gel Technology, a Novel Agent That Inhibits Biofilm Development by Gram-Positive and Gram-Negative Wound Pathogens journal March 2014
Microbiology of the skin and the role of biofilms in infection journal October 2011
High-energy ball milling technique for ZnO nanoparticles as antibacterial material journal April 2011
Effect of azithromycin and clarithromycin therapy on pharyngeal carriage of macrolide-resistant streptococci in healthy volunteers: a randomised, double-blind, placebo-controlled study journal February 2007
The ability of a colloidal silver gel wound dressing to kill bacteria in vitro and in vivo journal April 2017
Impact of Silver-Containing Wound Dressings on Bacterial Biofilm Viability and Susceptibility to Antibiotics during Prolonged Treatment journal September 2010
Sol-gel-derived c-axis oriented ZnO thin films journal January 1998
Biofilms as Complex Differentiated Communities journal October 2002
The lattice relaxation of ZnO single crystal (0001) surface journal June 2000
Effect of lattice constant of zinc oxide on antibacterial characteristics journal August 2004
Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model journal December 2009
Factors Affecting Wound Healing journal February 2010
Mechanisms of antibiotic resistance in bacterial biofilms journal January 2002
Burn Wound Infections journal April 2006
Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms journal June 2002

Figures / Tables (10)

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ZnS particles
anti-biofilm
Staphylococcus aureus
anti-MRSA
cryomilling