Characterization and engineering of a plastic-degrading aromatic polyesterase

Austin, Harry P.; Allen, Mark D.; Donohoe, Bryon S.; Rorrer, Nicholas A.; Kearns, Fiona L.; Silveira, Rodrigo L.; Pollard, Benjamin C.; Dominick, Graham; Duman, Ramona; El Omari, Kamel; Mykhaylyk, Vitaliy; Wagner, Armin; Michener, William E.; Amore, Antonella; Skaf, Munir S.; Crowley, Michael F.; Thorne, Alan W.; Johnson, Christopher W.; Woodcock, H. Lee; McGeehan, John E.; Beckham, Gregg T.

doi:10.1073/pnas.1718804115

Characterization and engineering of a plastic-degrading aromatic polyesterase

Journal Article · Tue Apr 17 04:00:00 EDT 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1718804115· OSTI ID:1433403

Austin, Harry P. ^[1]; Allen, Mark D. ^[1]; ^[2]; Rorrer, Nicholas A. ^[3]; Kearns, Fiona L. ^[4]; Silveira, Rodrigo L. ^[5]; Pollard, Benjamin C. ^[4]; Dominick, Graham ^[3]; ^[6]; El Omari, Kamel ^[6]; Mykhaylyk, Vitaliy ^[6]; ^[6]; Michener, William E. ^[3]; Amore, Antonella ^[2]; Skaf, Munir S. ^[7]; Crowley, Michael F. ^[2]; Thorne, Alan W. ^[1]; Johnson, Christopher W. ^[3]; Woodcock, H. Lee ^[4]; ^[1] more »

Molecular Biophysics Laboratories, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom,
Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401,
National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401,
Department of Chemistry, University of South Florida, Tampa, FL 33620-5250,
Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401,, Institute of Chemistry, University of Campinas, Campinas, 13083-970 Sao Paulo, Brazil,
Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom
Institute of Chemistry, University of Campinas, Campinas, 13083-970 Sao Paulo, Brazil,

Significance

Synthetic polymers are ubiquitous in the modern world but pose a global environmental problem. While plastics such as poly(ethylene terephthalate) (PET) are highly versatile, their resistance to natural degradation presents a serious, growing risk to fauna and flora, particularly in marine environments. Here, we have characterized the 3D structure of a newly discovered enzyme that can digest highly crystalline PET, the primary material used in the manufacture of single-use plastic beverage bottles, in some clothing, and in carpets. We engineer this enzyme for improved PET degradation capacity and further demonstrate that it can also degrade an important PET replacement, polyethylene-2,5-furandicarboxylate, providing new opportunities for biobased plastics recycling.

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Sponsoring Organization:: USDOE

OSTI ID:: 1433403

Alternate ID(s):: OSTI ID: 1435703

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 19 Vol. 115; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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