Conformation of methylated GGQ in the Peptidyl Transferase Center during Translation Termination

Zeng, Fuxing; Jin, Hong

doi:10.1038/s41598-018-20107-8

Conformation of methylated GGQ in the Peptidyl Transferase Center during Translation Termination

Journal Article · Mon Feb 05 00:00:00 EST 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-20107-8· OSTI ID:1432880

Zeng, Fuxing ^[1]; Jin, Hong ^[1]

Univ. of Illinois at Urbana-Champaign, IL (United States)

The universally conserved Gly-Gly-Gln (GGQ) tripeptide in release factors or release factor-like surveillance proteins is required to catalyze the release of nascent peptide in the ribosome. The glutamine of the GGQ is methylated post-translationally at the N⁵ position in vivo; this covalent modification is essential for optimal cell growth and efficient translation termination. However, the precise conformation of the methylated-GGQ tripeptide in the ribosome remains unknown. Using cryoEM and X-ray crystallography, we report the conformation of methylated-GGQ in the peptidyl transferase center of the ribosome during canonical translational termination and co-translation quality control. It has been suggested that the GGQ motif arose independently through convergent evolution among otherwise unrelated proteins that catalyze peptide release. The requirement for this tripeptide in the highly conserved peptidyl transferase center suggests that the conformation reported here is likely shared during termination of protein synthesis in all domains of life

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH)

OSTI ID:: 1432880

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 8; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
Cryoelectron microscopy
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Conformation of methylated GGQ in the Peptidyl Transferase Center during Translation Termination

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