Expression system for structural and functional studies of human glycosylation enzymes

Moremen, Kelley W.; Ramiah, Annapoorani; Stuart, Melissa; Steel, Jason; Meng, Lu; Forouhar, Farhad; Moniz, Heather A.; Gahlay, Gagandeep; Gao, Zhongwei; Chapla, Digantkumar; Wang, Shuo; Yang, Jeong-Yeh; Prabhakar, Pradeep Kumar; Johnson, Roy; Rosa, Mitche dela; Geisler, Christoph; Nairn, Alison V.; Seetharaman, Jayaraman; Wu, Sheng-Cheng; Tong, Liang; Gilbert, Harry J.; LaBaer, Joshua; Jarvis, Donald L.

doi:10.1038/nchembio.2539

Expression system for structural and functional studies of human glycosylation enzymes

Journal Article · Mon Dec 18 00:00:00 EST 2017 · Nature Chemical Biology

DOI:https://doi.org/10.1038/nchembio.2539· OSTI ID:1432876

Moremen, Kelley W. ^[1]; Ramiah, Annapoorani ^[1]; Stuart, Melissa ^[2]; Steel, Jason ^[3]; Meng, Lu ^[1]; Forouhar, Farhad ^[4]; Moniz, Heather A. ^[1]; Gahlay, Gagandeep ^[2]; Gao, Zhongwei ^[1]; Chapla, Digantkumar ^[1]; Wang, Shuo ^[1]; Yang, Jeong-Yeh ^[1]; Prabhakar, Pradeep Kumar ^[1]; Johnson, Roy ^[1]; Rosa, Mitche dela ^[1]; Geisler, Christoph ^[2]; Nairn, Alison V. ^[1]; Seetharaman, Jayaraman ^[4]; Wu, Sheng-Cheng ^[1]; ^[4] more »

Univ. of Georgia, Athens, GA (United States)
Univ. of Wyoming, Laramie, WY (United States)
Arizona State Univ., Tempe, AZ (United States)
Columbia Univ., New York, NY (United States)
Univ. of Georgia, Athens, GA (United States); Newcastle Univ., Newcastle upon Tyne (United Kingdom)

Vertebrate glycoproteins and glycolipids are synthesized in complex biosynthetic pathways localized predominantly within membrane compartments of the secretory pathway. The enzymes that catalyze these reactions are exquisitely specific, yet few have been extensively characterized because of challenges associated with their recombinant expression as functional products. We used a modular approach to create an expression vector library encoding all known human glycosyltransferases, glycoside hydrolases, and sulfotransferases, as well as other glycan-modifying enzymes. We then expressed the enzymes as secreted catalytic domain fusion proteins in mammalian and insect cell hosts, purified and characterized a subset of the enzymes, and determined the structure of one enzyme, the sialyltransferase ST6GalNAcII. Many enzymes were produced at high yields and at similar levels in both hosts, but individual protein expression levels varied widely. Furthermore, this expression vector library will be a transformative resource for recombinant enzyme production, broadly enabling structure–function studies and expanding applications of these enzymes in glycochemistry and glycobiology.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: NIH

OSTI ID:: 1432876

Journal Information:: Nature Chemical Biology, Journal Name: Nature Chemical Biology Journal Issue: 2 Vol. 14; ISSN 1552-4450

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (57)

Selective Exo-Enzymatic Labeling of N-Glycans on the Surface of Living Cells by Recombinant ST6Gal I Mbua, Ngalle Eric; Li, Xiuru; Flanagan-Steet, Heather R. Angewandte Chemie, Vol. 125, Issue 49 https://doi.org/10.1002/ange.201307095	journal	October 2013
Selective Exo-Enzymatic Labeling of N-Glycans on the Surface of Living Cells by Recombinant ST6Gal I Mbua, Ngalle Eric; Li, Xiuru; Flanagan-Steet, Heather R. Angewandte Chemie International Edition, Vol. 52, Issue 49 https://doi.org/10.1002/anie.201307095	journal	October 2013
A PEGylated Photocleavable Auxiliary Mediates the Sequential Enzymatic Glycosylation and Native Chemical Ligation of Peptides Bello, Claudia; Wang, Shuo; Meng, Lu Angewandte Chemie International Edition, Vol. 54, Issue 26 https://doi.org/10.1002/anie.201501517	journal	May 2015
Divergent Chemoenzymatic Synthesis of Asymmetrical-Core-Fucosylated and Core-Unmodified N -Glycans Li, Tiehai; Huang, Min; Liu, Lin Chemistry - A European Journal, Vol. 22, Issue 52 https://doi.org/10.1002/chem.201604999	journal	November 2016
Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the β1,4-galactosyltransferase-I11Edited by R. Huber Ramakrishnan, B.; Qasba, Pradman K. Journal of Molecular Biology, Vol. 310, Issue 1 https://doi.org/10.1006/jmbi.2001.4757	journal	June 2001
XtalView/Xfit—A Versatile Program for Manipulating Atomic Coordinates and Electron Density McRee, Duncan E. Journal of Structural Biology, Vol. 125, Issue 2-3 https://doi.org/10.1006/jsbi.1999.4094	journal	April 1999
Glycosyltransferase complexes in eukaryotes: long-known, prevalent but still unrecognized Kellokumpu, Sakari; Hassinen, Antti; Glumoff, Tuomo Cellular and Molecular Life Sciences, Vol. 73, Issue 2 https://doi.org/10.1007/s00018-015-2066-0	journal	October 2015
Targeting of proteins to the Golgi apparatus Gleeson, Paul A. Histochemistry and Cell Biology, Vol. 109, Issue 5-6 https://doi.org/10.1007/s004180050252	journal	June 1998
Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide Tessier, Daniel C.; Thomas, David Y.; Khouri, Henry E. Gene, Vol. 98, Issue 2 https://doi.org/10.1016/0378-1119(91)90171-7	journal	February 1991
Polymerase chain reaction cloning of a developmentally regulated member of the sialyltransferase gene family Livingston, B. D.; Paulson, J. C. Journal of Biological Chemistry, Vol. 268, Issue 16 https://doi.org/10.1016/S0021-9258(19)50227-7	journal	June 1993
Glycosyltransferases Paulson, J. C.; Colley, K. J. Journal of Biological Chemistry, Vol. 264, Issue 30 https://doi.org/10.1016/S0021-9258(19)84610-0	journal	October 1989
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
A deliberate approach to screening for initial crystallization conditions of biological macromolecules Luft, Joseph R.; Collins, Robert J.; Fehrman, Nancy A. Journal of Structural Biology, Vol. 142, Issue 1 https://doi.org/10.1016/S1047-8477(03)00048-0	journal	April 2003
Acceptor specificities and selective inhibition of recombinant human Gal- and GlcNAc-transferases that synthesize core structures 1, 2, 3 and 4 of O-glycans Gao, Yin; Aryal, Rajindra P.; Ju, Tongzhong Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1830, Issue 8 https://doi.org/10.1016/j.bbagen.2013.04.001	journal	August 2013
Sialylation of IgG antibodies inhibits IgG-mediated allergic reactions Epp, Alexandra; Hobusch, Juliane; Bartsch, Yannic C. Journal of Allergy and Clinical Immunology, Vol. 141, Issue 1 https://doi.org/10.1016/j.jaci.2017.06.021	journal	January 2018
NMR Characterization of Immunoglobulin G Fc Glycan Motion on Enzymatic Sialylation Barb, Adam W.; Meng, Lu; Gao, Zhongwei Biochemistry, Vol. 51, Issue 22 https://doi.org/10.1021/bi300319q	journal	May 2012
One-Step Selective Exoenzymatic Labeling (SEEL) Strategy for the Biotinylation and Identification of Glycoproteins of Living Cells Sun, Tiantian; Yu, Seok-Ho; Zhao, Peng Journal of the American Chemical Society, Vol. 138, Issue 36 https://doi.org/10.1021/jacs.6b04049	journal	September 2016
Super-resolution biomolecular crystallography with low-resolution data Schröder, Gunnar F.; Levitt, Michael; Brunger, Axel T. Nature, Vol. 464, Issue 7292 https://doi.org/10.1038/nature08892	journal	April 2010
Engineering and characterization of a superfolder green fluorescent protein Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy Nature Biotechnology, Vol. 24, Issue 1, p. 79-88 https://doi.org/10.1038/nbt1172	journal	December 2005
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins Schmidt, Thomas GM; Skerra, Arne Nature Protocols, Vol. 2, Issue 6 https://doi.org/10.1038/nprot.2007.209	journal	June 2007
Vertebrate protein glycosylation: diversity, synthesis and function Moremen, Kelley W.; Tiemeyer, Michael; Nairn, Alison V. Nature Reviews Molecular Cell Biology, Vol. 13, Issue 7 https://doi.org/10.1038/nrm3383	journal	June 2012
Structural insight into mammalian sialyltransferases Rao, Francesco V.; Rich, Jamie R.; Rakić, Bojana Nature Structural & Molecular Biology, Vol. 16, Issue 11 https://doi.org/10.1038/nsmb.1685	journal	October 2009
Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation Volkers, Gesa; Worrall, Liam J.; Kwan, David H. Nature Structural & Molecular Biology, Vol. 22, Issue 8 https://doi.org/10.1038/nsmb.3060	journal	July 2015
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
The repertoire of glycan determinants in the human glycome Cummings, Richard D. Molecular BioSystems, Vol. 5, Issue 10 https://doi.org/10.1039/b907931a	journal	January 2009
Synthesis of asymmetrical multiantennary human milk oligosaccharides Prudden, Anthony R.; Liu, Lin; Capicciotti, Chantelle J. Proceedings of the National Academy of Sciences, Vol. 114, Issue 27 https://doi.org/10.1073/pnas.1701785114	journal	June 2017
Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line Reeves, P. J.; Callewaert, N.; Contreras, R. Proceedings of the National Academy of Sciences, Vol. 99, Issue 21 https://doi.org/10.1073/pnas.212519299	journal	October 2002
A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Carrington, J. C.; Dougherty, W. G. Proceedings of the National Academy of Sciences, Vol. 85, Issue 10 https://doi.org/10.1073/pnas.85.10.3391	journal	May 1988
The Sialyltransferase Sialylmotif Participates in Binding the Donor Substrate CMP-NeuAc Datta, Arun K.; Paulson, James C. Journal of Biological Chemistry, Vol. 270, Issue 4 https://doi.org/10.1074/jbc.270.4.1497	journal	January 1995
The Lectin Domain of the Polypeptide GalNAc Transferase Family of Glycosyltransferases (ppGalNAc Ts) Acts as a Switch Directing Glycopeptide Substrate Glycosylation in an N- or C-terminal Direction, Further Controlling Mucin Type O -Glycosylation Gerken, Thomas A.; Revoredo, Leslie; Thome, Joseph J. C. Journal of Biological Chemistry, Vol. 288, Issue 27 https://doi.org/10.1074/jbc.M113.477877	journal	May 2013
Enzymatic Basis for N -Glycan Sialylation : STRUCTURE OF RAT α2,6-SIALYLTRANSFERASE (ST6GAL1) REVEALS CONSERVED AND UNIQUE FEATURES FOR GLYCAN SIALYLATION Meng, Lu; Forouhar, Farhad; Thieker, David Journal of Biological Chemistry, Vol. 288, Issue 48 https://doi.org/10.1074/jbc.M113.519041	journal	October 2013
Selective Exo-Enzymatic Labeling Detects Increased Cell Surface Sialoglycoprotein Expression upon Megakaryocytic Differentiation Yu, Seok-Ho; Zhao, Peng; Sun, Tiantian Journal of Biological Chemistry, Vol. 291, Issue 8 https://doi.org/10.1074/jbc.M115.700369	journal	January 2016
Protein O -Linked Mannose β-1,4- N -Acetylglucosaminyl-transferase 2 (POMGNT2) Is a Gatekeeper Enzyme for Functional Glycosylation of α-Dystroglycan Halmo, Stephanie M.; Singh, Danish; Patel, Sneha Journal of Biological Chemistry, Vol. 292, Issue 6 https://doi.org/10.1074/jbc.M116.764712	journal	December 2016
KDEL and KKXX Retrieval Signals Appended to the Same Reporter Protein Determine Different Trafficking between Endoplasmic Reticulum, Intermediate Compartment, and Golgi Complex Stornaiuolo, Mariano; Lotti, Lavinia V.; Borgese, Nica Molecular Biology of the Cell, Vol. 14, Issue 3 https://doi.org/10.1091/mbc.e02-08-0468	journal	March 2003
Cloning, expression, purification, and characterization of the acid -mannosidase from Trypanosoma cruzi Vandersall-Nairn, A. S.; Merkle, R. K.; O'Brien, K. Glycobiology, Vol. 8, Issue 12 https://doi.org/10.1093/glycob/8.12.1183	journal	December 1998
Mucin-type O-glycosylation is controlled by short- and long-range glycopeptide substrate recognition that varies among members of the polypeptide GalNAc transferase family Revoredo, Leslie; Wang, Shengjun; Bennett, Eric Paul Glycobiology, Vol. 26, Issue 4 https://doi.org/10.1093/glycob/cwv108	journal	November 2015
Biological roles of glycans Varki, Ajit Glycobiology, Vol. 27, Issue 1 https://doi.org/10.1093/glycob/cww086	journal	August 2016
Rapid screening of sugar-nucleotide donor specificities of putative glycosyltransferases Sheikh, M. Osman; Halmo, Stephanie M.; Patel, Sneha Glycobiology, Vol. 27, Issue 3 https://doi.org/10.1093/glycob/cww114	journal	December 2016
UniProt: the Universal Protein knowledgebase Apweiler, R. Nucleic Acids Research, Vol. 32, Issue 90001 https://doi.org/10.1093/nar/gkh131	journal	January 2004
The carbohydrate-active enzymes database (CAZy) in 2013 Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1178	journal	November 2013
UniProt: the universal protein knowledgebase No authors listed Nucleic Acids Research, Vol. 45, Issue D1, p. D158-D169 https://doi.org/10.1093/nar/gkw1099	journal	November 2016
UniProt: the universal protein knowledgebase UniProt Consortium, The Nucleic Acids Research, Vol. 46, Issue 5 https://doi.org/10.1093/nar/gky092	journal	February 2018
A short history of SHELX Sheldrick, George M. Acta Crystallographica Section A Foundations of Crystallography, Vol. 64, Issue 1, p. 112-122 https://doi.org/10.1107/S0108767307043930	journal	December 2007
COMO : a program for combined molecular replacement Jogl, Gerwald; Tao, Xiao; Xu, Yingwu Acta Crystallographica Section D Biological Crystallography, Vol. 57, Issue 8 https://doi.org/10.1107/S0907444901006783	journal	July 2001
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
The structure of human α-2,6-sialyltransferase reveals the binding mode of complex glycans Kuhn, Bernd; Benz, Jörg; Greif, Michael Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 9 https://doi.org/10.1107/S0907444913015412	journal	August 2013
A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation Beckett, Dorothy; Kovaleva, Elena; Schatz, Peter J. Protein Science, Vol. 8, Issue 4 https://doi.org/10.1110/ps.8.4.921	journal	January 1999
Expression of a Recombinant Human Glycosyltransferase from a Synthetic Gene and its Utilization for Synthesis of the Human Blood Group B Trisaccharide Seto, Nina O. L.; Palcic, Monica M.; Hindsgaul, Ole European Journal of Biochemistry, Vol. 234, Issue 1 https://doi.org/10.1111/j.1432-1033.1995.323_c.x	journal	November 1995
Two Arabidopsis proteins synthesize acetylated xylan in vitro Urbanowicz, Breeanna R.; Peña, Maria J.; Moniz, Heather A. The Plant Journal, Vol. 80, Issue 2 https://doi.org/10.1111/tpj.12643	journal	September 2014
Structural, mutagenic and in silico studies of xyloglucan fucosylation in Arabidopsis thaliana suggest a water-mediated mechanism Urbanowicz, Breeanna R.; Bharadwaj, Vivek S.; Alahuhta, Markus The Plant Journal, Vol. 91, Issue 6 https://doi.org/10.1111/tpj.13628	journal	August 2017
Structural basis for regulation of human calcium-sensing receptor by magnesium ions and an unexpected tryptophan derivative co-agonist Zhang, Chen; Zhang, Tuo; Zou, Juan Science Advances, Vol. 2, Issue 5 https://doi.org/10.1126/sciadv.1600241	journal	May 2016
Glycosyltransferases: Structures, Functions, and Mechanisms Lairson, L. L.; Henrissat, B.; Davies, G. J. Annual Review of Biochemistry, Vol. 77, Issue 1 https://doi.org/10.1146/annurev.biochem.76.061005.092322	journal	June 2008
Role of the Human ST6GalNAc-I and ST6GalNAc-II in the Synthesis of the Cancer-Associated Sialyl-Tn Antigen Marcos, Nuno T.; Pinho, Sandra; Grandela, Catarina Cancer Research, Vol. 64, Issue 19 https://doi.org/10.1158/0008-5472.CAN-04-1921	journal	October 2004
Biochemical characterization of functional domains of the chaperone Cosmc Hanes, Melinda S.; Moremen, Kelley W.; Cummings, Richard D. PLOS ONE, Vol. 12, Issue 6 https://doi.org/10.1371/journal.pone.0180242	journal	June 2017
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan Praissman, Jeremy L.; Live, David H.; Wang, Shuo eLife, Vol. 3 https://doi.org/10.7554/eLife.03943	journal	October 2014
The functional O-mannose glycan on α-dystroglycan contains a phospho-ribitol primed for matriglycan addition Praissman, Jeremy L.; Willer, Tobias; Sheikh, M. Osman eLife, Vol. 5 https://doi.org/10.7554/eLife.14473	journal	April 2016

Cited By (27)

N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses Broszeit, Frederik; Tzarum, Netanel; Zhu, Xueyong Cell Reports, Vol. 27, Issue 11 https://doi.org/10.1016/j.celrep.2019.05.048	journal	June 2019
Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase García-García, Ana; Ceballos-Laita, Laura; Serna, Sonia Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14794-z	journal	February 2020
An automated platform for the enzyme-mediated assembly of complex oligosaccharides Li, Tiehai; Liu, Lin; Wei, Na Nature Chemistry, Vol. 11, Issue 3 https://doi.org/10.1038/s41557-019-0219-8	journal	February 2019
Structural and functional role of disulphide bonds and substrate binding residues of the human beta-galactoside alpha-2,3-sialyltransferase 1 (hST3Gal1) Ortiz-Soto, Maria Elena; Reising, Sabine; Schlosser, Andreas Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-54384-8	journal	November 2019
Critical Review of Plant Cell Wall Matrix Polysaccharide Glycosyltransferase Activities Verified by Heterologous Protein Expression Amos, Robert A.; Mohnen, Debra Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00915	journal	July 2019
Novel Zebrafish Mono-α2,8-sialyltransferase (ST8Sia VIII): An Evolutionary Perspective of α2,8-Sialylation Chang, Lan-Yi; Teppa, Elin; Noel, Maxence International Journal of Molecular Sciences, Vol. 20, Issue 3 https://doi.org/10.3390/ijms20030622	journal	January 2019
Additional file 2 of The O-glycosylating enzyme GALNT2 acts as an oncogenic driver in non-small cell lung cancer Hu, Qing; Tian, Tian; Leng, Yahui figshare https://doi.org/10.6084/m9.figshare.20933520.v1	dataset	January 2022
Additional file 3 of The O-glycosylating enzyme GALNT2 acts as an oncogenic driver in non-small cell lung cancer Hu, Qing; Tian, Tian; Leng, Yahui figshare https://doi.org/10.6084/m9.figshare.20933523	dataset	January 2022
Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases Houeix, Benoit; Cairns, Michael T. PeerJ, Vol. 7 https://doi.org/10.7717/peerj.5788	journal	January 2019
Toward Automated Enzymatic Glycan Synthesis in a Compartmented Flow Microreactor System Heinzler, Raphael; Fischöder, Thomas; Elling, Lothar Advanced Synthesis & Catalysis, Vol. 361, Issue 19 https://doi.org/10.1002/adsc.201900709	journal	August 2019
An Enzymatic N‐Acylation Step Enables the Biocatalytic Synthesis of Unnatural Sialosides Laborda, Pedro; Lyu, Yong‐Mei; Parmeggiani, Fabio Angewandte Chemie, Vol. 132, Issue 13 https://doi.org/10.1002/ange.201914338	journal	February 2020
An Enzymatic N‐Acylation Step Enables the Biocatalytic Synthesis of Unnatural Sialosides Laborda, Pedro; Lyu, Yong‐Mei; Parmeggiani, Fabio Angewandte Chemie International Edition, Vol. 59, Issue 13 https://doi.org/10.1002/anie.201914338	journal	March 2020
Cell-based glycan arrays for probing glycan–glycan binding protein interactions Briard, Jennie Grace; Jiang, Hao; Moremen, Kelley W. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-03245-5	journal	February 2018
Streamlining the chemoenzymatic synthesis of complex N-glycans by a stop and go strategy Liu, Lin; Prudden, Anthony R.; Capicciotti, Chantelle J. Nature Chemistry, Vol. 11, Issue 2 https://doi.org/10.1038/s41557-018-0188-3	journal	December 2018
Legionella effector SetA as a general O-glucosyltransferase for eukaryotic proteins Gao, Ling; Song, Qitao; Liang, Hao Nature Chemical Biology, Vol. 15, Issue 3 https://doi.org/10.1038/s41589-018-0189-y	journal	January 2019
Emerging structural insights into glycosyltransferase-mediated synthesis of glycans Moremen, Kelley W.; Haltiwanger, Robert S. Nature Chemical Biology, Vol. 15, Issue 9 https://doi.org/10.1038/s41589-019-0350-2	journal	August 2019
Reprogramming the enzymatic assembly line for site-specific fucosylation Ye, Jinfeng; Xia, Hui; Sun, Na Nature Catalysis, Vol. 2, Issue 6 https://doi.org/10.1038/s41929-019-0281-z	journal	May 2019
Carbohydrates@MOFs Astria, Efwita; Thonhofer, Martin; Ricco, Raffaele Materials Horizons, Vol. 6, Issue 5 https://doi.org/10.1039/c8mh01611a	journal	January 2019
Chemoenzymatic synthesis of the oligosaccharide moiety of the tumor-associated antigen disialosyl globopentaosylceramide 't Hart, Ingrid M. E.; Li, Tiehai; Wolfert, Margreet A. Organic & Biomolecular Chemistry, Vol. 17, Issue 31 https://doi.org/10.1039/c9ob01368g	journal	January 2019
Human N -acetylglucosaminyltransferase II substrate recognition uses a modular architecture that includes a convergent exosite Kadirvelraj, Renuka; Yang, Jeong-Yeh; Sanders, Justin H. Proceedings of the National Academy of Sciences, Vol. 115, Issue 18 https://doi.org/10.1073/pnas.1716988115	journal	April 2018
A two-phase model for the non-processive biosynthesis of homogalacturonan polysaccharides by the GAUT1:GAUT7 complex Amos, Robert A.; Pattathil, Sivakumar; Yang, Jeong-Yeh Journal of Biological Chemistry, Vol. 293, Issue 49 https://doi.org/10.1074/jbc.ra118.004463	journal	October 2018
A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation Steentoft, Catharina; Yang, Zhang; Wang, Shengjun Glycobiology, Vol. 29, Issue 9 https://doi.org/10.1093/glycob/cwz041	journal	June 2019
Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O -Methylated Plant Polysaccharides Smith, Peter J.; O’Neill, Malcolm A.; Backe, Jason SLAS TECHNOLOGY: Translating Life Sciences Innovation, Vol. 25, Issue 4 https://doi.org/10.1177/2472630320912692	journal	March 2020
Toward Automated Enzymatic Glycan Synthesis in a Compartmented Flow Microreactor System Heinzler, Raphael; Fischöder, Thomas; Elling, Lothar RWTH Aachen University https://doi.org/10.18154/rwth-2019-08207	text	January 2019
Glycosylation Flux Analysis of Immunoglobulin G in Chinese Hamster Ovary Perfusion Cell Culture Hutter, Sandro; Wolf, Moritz; Papili Gao, Nan Processes, Vol. 6, Issue 10 https://doi.org/10.3390/pr6100176	journal	October 2018
Glycosylation Flux Analysis of Immunoglobulin G in Chinese Hamster Ovary Perfusion Cell Culture Hutter, Sandro; Wolf, Moritz; Papili Gao, Nan ETH Zurich https://doi.org/10.3929/ethz-b-000294344	text	January 2018
Toward Automated Enzymatic Glycan Synthesis in a Compartmented Flow Microreactor System Heinzler, Raphael; Fischöder, T.; Elling, L. Karlsruhe https://doi.org/10.5445/ir/1000098147	text	January 2019

Similar Records

Producing a glycosylating Escherichia coli cell factory: The placement of the bacterial oligosaccharyl transferase pglB onto the genome

Journal Article · Sun Jan 14 23:00:00 EST 2018 · Biochemical and Biophysical Research Communications · OSTI ID:23100614

Human N-acetylglucosaminyltransferase II substrate recognition uses a modular architecture that includes a convergent exosite

Journal Article · Sun Apr 15 20:00:00 EDT 2018 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1464834

Harnessing Glycosylation to Improve Cellulase Activity

Journal Article · Fri Jun 01 00:00:00 EDT 2012 · Current Opinion in Biotechnology · OSTI ID:1045063

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Carbohydrates
Glycobiololgy
Post-translational modifications
Structure determination

Expression system for structural and functional studies of human glycosylation enzymes

Citation Formats

References (57)

Cited By (27)

Similar Records

Related Subjects