Fermentation of dihydroxyacetone by engineered Escherichia coli and Klebsiella variicola to products
Methane can be converted to triose dihydroxyacetone (DHA) by chemical processes with formaldehyde as an intermediate. Carbon dioxide, a by-product of various industries including ethanol/butanol biorefineries, can also be converted to formaldehyde and then to DHA. DHA, upon entry into a cell and phosphorylation to DHA-3-phosphate, enters the glycolytic pathway and can be fermented to any one of several products. However, DHA is inhibitory to microbes due to its chemical interaction with cellular components. Fermentation of DHA tod-lactate byEscherichia colistrain TG113 was inefficient, and growth was inhibited by 30 g∙L-1DHA. An ATP-dependent DHA kinase fromKlebsiella oxytoca(pDC117d) permitted growth of strain TG113 in a medium with 30 g∙L-1DHA, and in a fed-batch fermentation thed-lactate titer of TG113(pDC117d) was 580 ± 21 mM at a yield of 0.92 g∙g-1DHA fermented.Klebsiella variicolastrain LW225, with a higher glucose flux thanE. coli, produced 811 ± 26 mMd-lactic acid at an average volumetric productivity of 2.0 g-1∙L-1∙h-1. Fermentation of DHA required a balance between transport of the triose and utilization by the microorganism. Using other engineeredE. colistrains, we also fermented DHA to succinic acid and ethanol, demonstrating the potential of converting CH4and CO2to value-added chemicals and fuels by a combination of chemical/biological processes.
- Research Organization:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO); USDOE Office of Policy and International Affairs (PO); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
- Grant/Contract Number:
- PI0000031
- OSTI ID:
- 1432427
- Alternate ID(s):
- OSTI ID: 1540286; OSTI ID: 1766313
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 115 Journal Issue: 17; ISSN 0027-8424
- Publisher:
- National Academy of SciencesCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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