Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans

Khosravi, Claire; Battaglia, Evy; Kun, Roland S.; Dalhuijsen, Sacha; Visser, Jaap; Aguilar-Pontes, María Victoria; Zhou, Miaomiao; Heyman, Heino M.; Kim, Young-Mo; Baker, Scott E.; de Vries, Ronald P.

doi:10.1186/s12864-018-4609-x

Title: Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans

Journal Article · Thu Mar 22 00:00:00 EDT 2018 · BMC Genomics

DOI:https://doi.org/10.1186/s12864-018-4609-x· OSTI ID:1618548

Khosravi, Claire; Battaglia, Evy; Kun, Roland S.; Dalhuijsen, Sacha; Visser, Jaap; Aguilar-Pontes, María Victoria; Zhou, Miaomiao; Heyman, Heino M.; Kim, Young-Mo; Baker, Scott E.;

Background: Plant biomass is the most abundant carbon source for many fungal species. In the biobased industry fungi are used to produce lignocellulolytic enzymes to degrade agricultural waste biomass. Here we evaluated if it would be possible to create an Aspergillus nidulans strain that releases but does not metabolize hexoses from plant biomass. For this purpose, metabolic mutants were generated that were impaired in glycolysis, by using hexokinase (hxkA) and glucokinase (glkA) negative strains. To prevent repression of enzyme production due to the hexose accumulation, strains were generated that combined these mutations with a deletion in creA, the repressor involved in regulating preferential use of different carbon catabolic pathways. Results: Phenotypic analysis revealed reduced growth for the hxkA1 glkA4 mutant on wheat bran. However, hexoses did not accumulate during growth of the mutants on wheat bran, suggesting that glucose metabolism is re-routed towards alternative carbon catabolic pathways. The creAΔ4 mutation in combination with preventing initial phosphorylation in glycolysis resulted in better growth than the hxkA/glkA mutant and an increased expression of pentose catabolic and pentose phosphate pathway genes. This indicates that the reduced ability to use hexoses as carbon sources created a shift towards the pentose fraction of wheat bran as a major carbon source to support growth. Conclusion: Blocking the direct entry of hexoses to glycolysis activates alternative metabolic conversion of these sugars in A. nidulans during growth on plant biomass, but also upregulates conversion of other sugars, such as pentoses.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0008744; AC05-76RL01830

OSTI ID:: 1618548

Alternate ID(s):: OSTI ID: 1431406

Report Number(s):: PNNL-SA-132841; 214; PII: 4609

Journal Information:: BMC Genomics, Journal Name: BMC Genomics Vol. 19 Journal Issue: 1; ISSN 1471-2164

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 8 works

Citation information provided by
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Additional file 10: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/M9.FIGSHARE.6020816 The current record is supplemented by this dataset	dataset	March 2018
Additional file 10: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020816.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 11: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020834 The current record is supplemented by this figure	figure	March 2018
Additional file 11: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020834.v1 The current record is supplemented by this figure	figure	March 2018
Additional file 1: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020849 The current record is supplemented by this dataset	dataset	March 2018
Additional file 1: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020849.v1 The current record is supplemented by this figure	figure	March 2018
Additional file 2: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/M9.FIGSHARE.6020882 The current record is supplemented by this dataset	dataset	March 2018
Additional file 2: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020882.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 3: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020894 The current record is supplemented by this dataset	dataset	March 2018
Additional file 3: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020894.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 4: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/M9.FIGSHARE.6020909 The current record is supplemented by this dataset	dataset	March 2018
Additional file 4: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020909.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 5: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020927.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 6: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020942 The current record is supplemented by this figure	figure	March 2018
Additional file 6: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020942.v1 The current record is supplemented by this figure	figure	March 2018
Additional file 7: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020963 The current record is supplemented by this dataset	dataset	March 2018
Additional file 7: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020963.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 8: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020981.v1 The current record is supplemented by this dataset	dataset	March 2018
Additional file 9: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020996 The current record is supplemented by this figure	figure	March 2018
Additional file 9: of Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.6020996.v1 The current record is supplemented by this dataset	dataset	March 2018
Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans Khosravi, Claire; Battaglia, Evy; Kun, Roland https://doi.org/10.6084/m9.figshare.c.4040144.v1 The current record is supplemented by this dataset	dataset	March 2018