The Kinetochore Receptor for the Cohesin Loading Complex

Hinshaw, Stephen M.; Makrantoni, Vasso; Harrison, Stephen C.; Marston, Adèle L.

doi:10.1016/j.cell.2017.08.017

The Kinetochore Receptor for the Cohesin Loading Complex

Journal Article · Thu Sep 21 00:00:00 EDT 2017 · Cell

DOI:https://doi.org/10.1016/j.cell.2017.08.017· OSTI ID:1430320

Hinshaw, Stephen M. ^[1]; Makrantoni, Vasso ^[2]; Harrison, Stephen C. ^[3]; Marston, Adèle L. ^[2]

Harvard Medical School, Boston, MA (United States)
Univ. of Edinburgh (United Kingdom)
Harvard Medical School, Boston, MA (United States); Howard Hughes Medical Inst., Chevy Chase, MD (United States)

The ring-shaped cohesin complex brings together distant DNA domains to maintain, express, and segregate the genome. Establishing specific chromosomal linkages depends on cohesin recruitment to defined loci. One such locus is the budding yeast centromere, which is a paradigm for targeted cohesin loading. The kinetochore, a multiprotein complex that connects centromeres to microtubules, drives the recruitment of high levels of cohesin to link sister chromatids together. We have exploited this system to determine the mechanism of specific cohesin recruitment. We show that phosphorylation of the Ctf19 kinetochore protein by a conserved kinase, DDK, provides a binding site for the Scc2/4 cohesin loading complex, thereby directing cohesin loading to centromeres. A similar mechanism targets cohesin to chromosomes in vertebrates. These findings represent a complete molecular description of targeted cohesin loading, a phenomenon with wide-ranging importance in chromosome segregation and, in multicellular organisms, transcription regulation.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: NIH; Wellcome Centre for Cell Biology

OSTI ID:: 1430320

Journal Information:: Cell, Journal Name: Cell Journal Issue: 1 Vol. 171; ISSN 0092-8674

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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