Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice

Lu, Kai; Wu, Bowen; Wang, Jie; Zhu, Wei; Nie, Haipeng; Qian, Junjie; Huang, Weiting; Fang, Zhongming

doi:10.1111/pbi.12907

Title: Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice

Journal Article · Sun Mar 25 00:00:00 EDT 2018 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12907· OSTI ID:1429543

Lu, Kai ^[1]; Wu, Bowen ^[1]; Wang, Jie ^[1]; Zhu, Wei ^[1]; Nie, Haipeng ^[2]; Qian, Junjie ^[2]; Huang, Weiting ^[1];

^[2]

Center of Applied Biotechnology Wuhan Institute of Bioengineering Wuhan China
Center of Applied Biotechnology Wuhan Institute of Bioengineering Wuhan China, National Key Laboratory of Crop Genetic Improvement College of Plant Science and Technology Huazhong Agricultural University Wuhan China

Summary Amino acid transporters ( AAT s) play indispensable roles in nutrient allocation during plant development. In this study, we demonstrated that inhibiting expression of the rice amino acid transporter Os AAP 3 increased grain yield due to a formation of larger numbers of tillers as a result of increased bud outgrowth. Elevated expression of Os AAP 3 in transgenic plants resulted in significantly higher amino acid concentrations of Lys, Arg, His, Asp, Ala, Gln, Gly, Thr and Tyr, and inhibited bud outgrowth and rice tillering. However, RNA i of Os AAP 3 decreased significantly Arg, Lys, Asp and Thr concentrations to a small extent, and thus promoted bud outgrowth, increased significantly tiller numbers and effective panicle numbers per plant, and further enhanced significantly grain yield and nitrogen use efficiency ( NUE ). The promoter sequences of Os AAP 3 showed some divergence between Japonica and Indica rice, and expression of the gene was higher in Japonica , which produced fewer tillers than Indica . We generated knockout lines of Os AAP 3 on Japonica ZH 11 and KY 131 using CRISPR technology and found that grain yield could be increased significantly. These results suggest that manipulation of Os AAP 3 expression could be used to increase grain yield in rice.

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Sponsoring Organization:: USDOE

OSTI ID:: 1429543

Alternate ID(s):: OSTI ID: 1429544

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 16 Journal Issue: 10; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 110 works

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