The terpene synthase gene family in Tripterygium wilfordii harbors a labdane-type diterpene synthase among the monoterpene synthase TPS-b subfamily

Hansen, Nikolaj L.; Heskes, Allison M.; Hamberger, Britta; Olsen, Carl E.; Hallstrom, Bjorn M.; Andersen-Ranberg, Johan; Hamberger, Bjorn

doi:10.1111/tpj.13410

Title: The terpene synthase gene family in Tripterygium wilfordii harbors a labdane-type diterpene synthase among the monoterpene synthase TPS-b subfamily

Journal Article · Tue Nov 01 00:00:00 EDT 2016 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.13410· OSTI ID:1427678

Hansen, Nikolaj L. ^[1]; Heskes, Allison M. ^[1]; Hamberger, Britta ^[1]; Olsen, Carl E. ^[2]; Hallstrom, Bjorn M. ^[3]; Andersen-Ranberg, Johan ^[1];

^[1]

Univ. of Copenhagen (Denmark). Plant Biochemistry Laboratory, Department of Plant and Environmental Sciences; Center for Synthetic Biology 'bioSYNergy' and Copenhagen Plant Sciences Centre, Copenhagen (Denmark)
Univ. of Copenhagen (Denmark). Plant Biochemistry Laboratory, Department of Plant and Environmental Sciences
Royal Institute of Technology, Stockholm (Sweden). Science for Life Laboratory, Kungliga Tekniska Högskolan

We present Tripterygium wilfordii (Celastraceae) is a medicinal plant with anti-inflammatory and immunosuppressive properties. Identification of a vast array of unusual sesquiterpenoids, diterpenoids and triterpenoids in T. wilfordii has spurred investigations of their pharmacological properties. The tri-epoxide lactone triptolide was the first of many diterpenoids identified, attracting interest due to the spectrum of bioactivities. To probe the genetic underpinning of diterpenoid diversity, an expansion of the class II diterpene synthase (diTPS) family was recently identified in a leaf transcriptome. Following detection of triptolide and simple diterpene scaffolds in the root, we sequenced and mined the root transcriptome. This allowed identification of the root-specific complement of TPSs and an expansion in the class I diTPS family. Functional characterization of the class II diTPSs established their activities in the formation of four C-20 diphosphate intermediates, precursors of both generalized and specialized metabolism and a novel scaffold for Celastraceae. Functional pairs of the class I and II enzymes resulted in formation of three scaffolds, accounting for some of the terpenoid diversity found in T. wilfordii. The absence of activity-forming abietane-type diterpenes encouraged further testing of TPSs outside the canonical class I diTPS family. TwTPS27, close relative of mono-TPSs, was found to couple with TwTPS9, converting normal-copalyl diphosphate to miltiradiene. The phylogenetic distance to established diTPSs indicates neo-functionalization of TwTPS27 into a diTPS, a function not previously observed in the TPS-b subfamily. Finally, this example of evolutionary convergence expands the functionality of TPSs in the TPS-b family and may contribute miltiradiene to the diterpenoids of T. wilfordii.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1427678

Journal Information:: The Plant Journal, Vol. 89, Issue 3; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 58 works

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Figures / Tables (19)

Supplemental table S1(p. 47)

Supplementary table S2(p. 48)

Supplemental table S3(p. 49)

Supplemental table S3 (continued)(p. 50)

Supplemental table S3 (continued)(p. 51)

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Tripterygium wilfordii
terpene synthase
specialized metabolism
convergent evolution
bioactive diterpenoids