skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A Portable Immunoassay Platform for Multiplexed Detection of Biotoxins in Clinical and Environmental Samples

Abstract

Multiple cases of attempted bioterrorism events using biotoxins have highlighted the urgent need for tools capable of rapid screening of suspect samples in the field (e.g., mailroom and public events). We present a portable microfluidic device capable of analyzing environmental (e.g., white powder), food (e.g., milk) and clinical (e.g., blood) samples for multiplexed detection of biotoxins. The device is rapid (<15-30 min sample-to-answer), sensitive (< 0.08 pg/mL detection limit for botulinum toxin), multiplexed (up to 64 parallel assays) and capable of analyzing small volume samples (< 20 μL total sample input). The immunoassay approach (SpinDx) is based on binding of toxins in a sample to antibody-laden capture particles followed by sedimentation of particles through a density-media in a microfluidic disk and quantification using a laser-induced fluorescence detector. A direct, blinded comparison with a gold standard ELISA revealed a 5-fold more sensitive detection limit for botulinum toxin while requiring 250-fold less sample volume and a 30 minute assay time with a near unity correlation. A key advantage of the technique is its compatibility with a variety of sample matrices with no additional sample preparation required. Ultrasensitive quantification has been demonstrated from direct analysis of multiple clinical, environmental and food samples, includingmore » white powder, whole blood, saliva, salad dressing, whole milk, peanut butter, half and half, honey, and canned meat. We believe that this device can met an urgent need in screening both potentially exposed people as well as suspicious samples in mail-rooms, airports, public sporting venues and emergency rooms. The general-purpose immunodiagnostics device can also find applications in screening of infectious and systemic diseases or serve as a lab device for conducting rapid immunoassays.« less

Authors:
 [1];  [2];  [3];  [4];  [4];  [5];  [5];  [3];  [1]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Cepheid, Sunnyvale, CA (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandstone Diagnostics, Livermore, CA (United States)
  4. US Dept. of Agriculture, Albany, CA (United States). Western Regional Research Center, Foodborne Contaminants Research Unit
  5. Univ. of Massachusetts, Dartmouth, MA (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
OSTI Identifier:
1427244
Report Number(s):
SAND-2015-0079J
Journal ID: ISSN 9999-0014; 558359
DOE Contract Number:  
AC04-94AL85000; U01A1075441; U54 AI065359
Resource Type:
Journal Article
Journal Name:
Sandia journal manuscript; Not yet accepted for publication
Additional Journal Information:
Journal Name: Sandia journal manuscript; Not yet accepted for publication; Journal ID: ISSN 9999-0014
Publisher:
Sandia
Country of Publication:
United States
Language:
English

Citation Formats

Koh, Chung-Yan, Piccini, Matthew Ernest, Schaff, Ulrich Y., Stanker, Larry H., Cheng, Luisa W., Ravichandran, Easwaran, Singh, Bal-Ram, Sommer, Greg J., and Singh, Anup K. A Portable Immunoassay Platform for Multiplexed Detection of Biotoxins in Clinical and Environmental Samples. United States: N. p., 2015. Web.
Koh, Chung-Yan, Piccini, Matthew Ernest, Schaff, Ulrich Y., Stanker, Larry H., Cheng, Luisa W., Ravichandran, Easwaran, Singh, Bal-Ram, Sommer, Greg J., & Singh, Anup K. A Portable Immunoassay Platform for Multiplexed Detection of Biotoxins in Clinical and Environmental Samples. United States.
Koh, Chung-Yan, Piccini, Matthew Ernest, Schaff, Ulrich Y., Stanker, Larry H., Cheng, Luisa W., Ravichandran, Easwaran, Singh, Bal-Ram, Sommer, Greg J., and Singh, Anup K. Thu . "A Portable Immunoassay Platform for Multiplexed Detection of Biotoxins in Clinical and Environmental Samples". United States. https://www.osti.gov/servlets/purl/1427244.
@article{osti_1427244,
title = {A Portable Immunoassay Platform for Multiplexed Detection of Biotoxins in Clinical and Environmental Samples},
author = {Koh, Chung-Yan and Piccini, Matthew Ernest and Schaff, Ulrich Y. and Stanker, Larry H. and Cheng, Luisa W. and Ravichandran, Easwaran and Singh, Bal-Ram and Sommer, Greg J. and Singh, Anup K.},
abstractNote = {Multiple cases of attempted bioterrorism events using biotoxins have highlighted the urgent need for tools capable of rapid screening of suspect samples in the field (e.g., mailroom and public events). We present a portable microfluidic device capable of analyzing environmental (e.g., white powder), food (e.g., milk) and clinical (e.g., blood) samples for multiplexed detection of biotoxins. The device is rapid (<15-30 min sample-to-answer), sensitive (< 0.08 pg/mL detection limit for botulinum toxin), multiplexed (up to 64 parallel assays) and capable of analyzing small volume samples (< 20 μL total sample input). The immunoassay approach (SpinDx) is based on binding of toxins in a sample to antibody-laden capture particles followed by sedimentation of particles through a density-media in a microfluidic disk and quantification using a laser-induced fluorescence detector. A direct, blinded comparison with a gold standard ELISA revealed a 5-fold more sensitive detection limit for botulinum toxin while requiring 250-fold less sample volume and a 30 minute assay time with a near unity correlation. A key advantage of the technique is its compatibility with a variety of sample matrices with no additional sample preparation required. Ultrasensitive quantification has been demonstrated from direct analysis of multiple clinical, environmental and food samples, including white powder, whole blood, saliva, salad dressing, whole milk, peanut butter, half and half, honey, and canned meat. We believe that this device can met an urgent need in screening both potentially exposed people as well as suspicious samples in mail-rooms, airports, public sporting venues and emergency rooms. The general-purpose immunodiagnostics device can also find applications in screening of infectious and systemic diseases or serve as a lab device for conducting rapid immunoassays.},
doi = {},
journal = {Sandia journal manuscript; Not yet accepted for publication},
issn = {9999-0014},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {1}
}