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Title: Mechanisms of proton relay and product release by Class A β-lactamase at ultrahigh resolution

Journal Article · · Federation of European Biochemical Societies (FEBS) Journal
DOI:https://doi.org/10.1111/febs.14315· OSTI ID:1426672
 [1];  [1];  [2];  [3];  [3];  [1]
  1. Univ. of South Florida, Tampa, FL (United States). College of Medicine, Dept. of Molecular Medicine
  2. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS). X-ray Science Division
  3. Univ. of Lodz (Poland). Dept. of Organic Chemistry, Faculty of Chemistry
+ Show Author Affiliations

The beta-lactam antibiotics inhibit penicillin-binding proteins (PBPs) by forming a stable, covalent, acyl-enzyme complex. During the evolution from PBPs to Class A beta-lactamases, the beta-lactamases acquired Glu166 to activate a catalytic water and cleave the acyl-enzyme bond. Here we present three product complex crystal structures of CTX-M-14 Class A beta-lactamase with a ruthenocene-conjugated penicillin-a 0.85 angstrom resolution structure of E166A mutant complexed with the penilloate product, a 1.30 angstrom resolution complex structure of the same mutant with the penicilloate product, and a 1.18 angstrom resolution complex structure of S70G mutant with a penicilloate product epimer-shedding light on the catalytic mechanisms and product inhibition of PBPs and Class A beta-lactamases. The E166A-penilloate complex captured the hydrogen bonding network following the protonation of the leaving group and, for the first time, unambiguously show that the ring nitrogen donates a proton to Ser130, which in turn donates a proton to Lys73. These observations indicate that in the absence of Glu166, the equivalent lysine would be neutral in PBPs and therefore capable of serving as the general base to activate the catalytic serine. Together with previous results, this structure suggests a common proton relay network shared by Class A beta-lactamases and PBPs, from the catalytic serine to the lysine, and ultimately to the ring nitrogen. Additionally, the E166A-penicilloate complex reveals previously unseen conformational changes of key catalytic residues during the release of the product, and is the first structure to capture the hydrolyzed product in the presence of an unmutated catalytic serine.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
National Institutes of Health (NIH); USDOE
Grant/Contract Number:
AC02-06CH11357; ACB‐12002; AGM‐12006; DEC‐2013/11/B/ST5/00997
OSTI ID:
1426672
Alternate ID(s):
OSTI ID: 1409859
Journal Information:
Federation of European Biochemical Societies (FEBS) Journal, Vol. 285, Issue 1; ISSN 1742-464X
Publisher:
Federation of European Biochemical SocietiesCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

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Cited By (1)

New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis journal January 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
X-ray crystallography
CTX-M
Penicillin Binding Protein
Penilloate
Penicilloate