Enhanced itaconic acid production in Aspergillus with increased LaeA expression

Dai, Ziyu; Baker, Scott E.

Enhanced itaconic acid production in Aspergillus with increased LaeA expression

Patent · Tue Mar 06 04:00:00 EST 2018

OSTI ID:1425892

Dai, Ziyu; Baker, Scott E.

Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 9,909,152

Application Number:: 14/928,511

OSTI ID:: 1425892

Country of Publication:: United States

Language:: English

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