skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study

Journal Article · · Scientific Reports
 [1];  [2];  [3];  [2];  [4];  [3];  [3];  [4];  [3];  [5]
  1. Huazhong Univ. of Science and Technology, Wuhan (China). School of Life Science and Technology; Texas A & M Univ., College Station, TX (United States). Dept. of Veterinary Pathology
  2. Huazhong Univ. of Science and Technology, Wuhan (China). School of Life Science and Technology; Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub. Dept. of Plant Pathology and Microbiology
  3. Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub. Dept. of Plant Pathology and Microbiology
  4. Huazhong Univ. of Science and Technology, Wuhan (China). School of Life Science and Technology
  5. Texas A & M Univ., College Station, TX (United States). Dept. of Veterinary Pathology. Office of Texas State Chemist
+ Show Author Affiliations

Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the application of shot-gun proteomic technology to study the bio-remediation of environmental hazards by white-rot fungus. Lignin, a naturally abundant component of the plant biomass, is discovered to promote the degradation of Azo dye by white-rot fungus Irpex lacteus CD2 in the lignin/dye/fungus system. Shotgun proteomics technique was used to understand degradation mechanism at the protein level for the lignin/dye/fungus system. Our proteomics study can identify about two thousand proteins (one third of the predicted white-rot fungal proteome) in a single experiment, as one of the most powerful proteomics platforms to study the fungal system to date. The study shows a significant enrichment of oxidoreduction functional category under the dye/lignin combined treatment. An in vitro validation is performed and supports our hypothesis that the synergy of Fenton reaction and manganese peroxidase might play an important role in DR5B dye degradation. The results could guide the development of effective bioremediation strategies and efficient lignocellulosic biomass conversion.

Research Organization:
Texas A & M Univ., College Station, TX (United States); Huazhong Univ. of Science and Technology, Wuhan (China)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; National Natural Science Foundation of China (NSFC); National High-tech Research and Development Program of China; Major State Basic Research Development Program of China
Grant/Contract Number:
EE0006112; EE0007104; 31170104; 2012AA101805; 2014CB138301
OSTI ID:
1425151
Journal Information:
Scientific Reports, Vol. 7; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 17 works
Citation information provided by
Web of Science

References (46)

The promoting effect of byproducts from Irpex lacteus on subsequent enzymatic hydrolysis of bio-pretreated cornstalks journal October 2011
Carcinogenicity of azo colorants: influence of solubility and bioavailability journal June 2004
The impact of proteomics on the understanding of functions and biogenesis of fungal extracellular vesicles journal January 2014
Influence of culture parameters on lignin metabolism byPhanerochaete chrysosporium journal June 1978
Proteomics of filamentous fungi journal September 2007
Genes associated with lignin degradation in the polyphagous white-rot pathogen Heterobasidion irregulare show substrate-specific regulation journal July 2013
Integrated analysis of shotgun proteomic data with PatternLab for proteomics 4.0 journal December 2015
Exploration of Natural Biomass Utilization Systems (NBUS) for advanced biofuel—from systems biology to synthetic design journal June 2014
Proteomics by Mass Spectrometry: Approaches, Advances, and Applications journal August 2009
Plant Protein-Protein Interaction Network and Interactome journal March 2010
Next-generation proteomics: towards an integrative view of proteome dynamics journal December 2012
White-rot fungi and their enzymes for the treatment of industrial dye effluents journal December 2003
WGCNA: an R package for weighted correlation network analysis journal December 2008
Genomic and molecular mechanisms for efficient biodegradation of aromatic dye journal January 2016
Bladder cancer among workers in the textile industry: Results of a spanish case-control study journal January 1988
Advances in fungal proteomics journal July 2007
SignalP 4.0: discriminating signal peptides from transmembrane regions journal September 2011
Elucidating the fungal stress response by proteomics journal January 2014
Fungal proteomics: from identification to function: Fungal proteomics journal May 2011
Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis journal March 2012
Proteomic analysis of mycelium and secretome of different Botrytis cinerea wild-type strains journal January 2014
Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment journal January 2013
Induction of Extracellular Hydroxyl Radical Production by White-Rot Fungi through Quinone Redox Cycling journal April 2009
Plant systems biology comes of age journal April 2008
ProLuCID: An improved SEQUEST-like algorithm with enhanced sensitivity and specificity journal November 2015
Cloning and Expression Analysis of a Novel Human Serine Hydrolase with Sequence Similarity to Prokaryotic Enzymes Involved in the Degradation of Aromatic Compounds journal May 1995
Applying mass spectrometry-based proteomics to genetics, genomics and network biology journal September 2009
Direct Cellular Lysis/Protein Extraction Protocol for Soil Metaproteomics journal December 2010
Proteomic and Metabolomic Analyses of the White-Rot Fungus Phanerochaete chrysosporium Exposed to Exogenous Benzoic Acid journal June 2008
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources journal December 2008
Methodologies and Perspectives of Proteomics Applied to Filamentous Fungi: From Sample Preparation to Secretome Analysis journal March 2015
Integration of shot-gun proteomics and bioinformatics analysis to explore plant hormone responses journal January 2012
Biodegradation of azo dyes Acid Red 183, Direct Blue 15 and Direct Red 75 by the isolate Penicillium oxalicum SAR-3 journal July 2014
Large-scale analysis of the yeast proteome by multidimensional protein identification technology journal March 2001
Cytoscape 2.8: new features for data integration and network visualization journal December 2010
Simultaneous conversion of all cell wall components by an oleaginous fungus without chemi-physical pretreatment journal January 2015
Analyzing Shotgun Proteomic Data with PatternLab for Proteomics journal June 2010
Induction, Purification and Characterization of a Novel Manganese Peroxidase from Irpex lacteus CD2 and Its Application in the Decolorization of Different Types of Dye journal November 2014
Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium journal April 2010
Fungal treatment of cornstalks enhances the delignification and xylan loss during mild alkaline pretreatment and enzymatic digestibility of glucan journal September 2010
Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures. journalarticle January 2018
Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion journal February 2009
Application of an Improved Proteomics Method for Abundant Protein Cleanup: Molecular and Genomic Mechanisms Study in Plant Defense journal August 2013
Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78 journal May 2004
Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa journal December 2009
Identification of a Serine Hydrolase Which Cleaves the Alicyclic Ring of Tetralin journal October 2000

Cited By (1)

Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies journal July 2019

Similar Records

Lytic polysaccharide monooxygenase synergized with lignin-degrading enzymes for efficient lignin degradation
Journal Article · Sat Sep 09 00:00:00 EDT 2023 · iScience · OSTI ID:1425151
+10 more
Differential Expression in Phanerochaete chrysosporium of Membrane-Associated Proteins Relevant to Lignin Degradation
Journal Article · Thu Oct 02 00:00:00 EDT 2008 · Applied and Environmental Microbiology, 74(23):7252-7257 · OSTI ID:1425151
+3 more
Multiple roles for lignin peroxidases in the biodegradation of organic pollutants
Conference · Sat Dec 31 00:00:00 EST 1994 · OSTI ID:1425151

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
proteomics
water microbiology