Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H

Ito, Fumiaki; Yang, Hanjing; Xiao, Xiao; Li, Shu-Xing; Wolfe, Aaron; Zirkle, Brett; Arutiunian, Vagan; Chen, Xiaojiang S.

doi:10.1038/s41598-018-21955-0

Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H

Journal Article · Tue Feb 27 23:00:00 EST 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-21955-0· OSTI ID:1424775

Ito, Fumiaki ^[1]; Yang, Hanjing ^[1]; ^[2]; Li, Shu-Xing ^[1]; Wolfe, Aaron ^[1]; Zirkle, Brett ^[1]; Arutiunian, Vagan ^[3]; Chen, Xiaojiang S. ^[1]

Univ. of Southern California, Los Angeles, CA (United States)
Merck Research Laboratories, Merck & Co., Inc, West Point, PA (United States); Univ. of Southern California, Los Angeles, CA (United States)
Meharry Medical College, Nashville, TN (United States); Univ. of Southern California, Los Angeles, CA (United States)

APOBEC3H (A3H) is a member of the APOBEC3 subfamily of DNA cytosine deaminases that are important for innate immune defense and have been implicated in cancer biogenesis. To understand the structural basis for A3H biochemical function, we determined a high-resolution structure of human A3H and performed extensive biochemical analysis. The 2.49 Å crystal structure reveals a uniquely long C-terminal helix 6 (h6), a disrupted β5 strand of the canonical five-stranded β-sheet core, and a long loop 1 around the Zn-active center. Mutation of a loop 7 residue, W115, disrupted the RNA-mediated dimerization of A3H yielding an RNA-free monomeric form that still possessed nucleic acid binding and deaminase activity. A3H expressed in HEK293T cells showed RNA dependent HMW complex formation and RNase A-dependent deaminase activity. A3H has a highly positively charged surface surrounding the Zn-active center, and multiple positively charged residues within this charged surface play an important role in the RNA-mediated HMW formation and deaminase inhibition. Furthermore, these positively charged residues affect subcellular localization of A3H between the nucleus and cytosol. Finally, we have identified multiple residues of loop 1 and 7 that contribute to the overall deaminase activity and the methylcytosine selectivity.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: NIH

OSTI ID:: 1424775

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 8; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (86)

APOBEC deaminases-mutases with defensive roles for immunity Prochnow, Courtney; Bransteitter, Ronda; Chen, XiaoJiang S. Science in China Series C: Life Sciences, Vol. 52, Issue 10 https://doi.org/10.1007/s11427-009-0133-1	journal	October 2009
Determinants of sequence-specificity within human AID and APOBEC3G Carpenter, Michael A.; Rajagurubandara, Erandi; Wijesinghe, Priyanga DNA Repair, Vol. 9, Issue 5 https://doi.org/10.1016/j.dnarep.2010.02.010	journal	May 2010
The Local Dinucleotide Preference of APOBEC3G Can Be Altered from 5′-CC to 5′-TC by a Single Amino Acid Substitution Rathore, Anurag; Carpenter, Michael A.; Demir, Özlem Journal of Molecular Biology, Vol. 425, Issue 22 https://doi.org/10.1016/j.jmb.2013.07.040	journal	November 2013
Multiple APOBEC3 Restriction Factors for HIV-1 and One Vif to Rule Them All Desimmie, Belete A.; Delviks-Frankenberrry, Krista A.; Burdick, Ryan C. Journal of Molecular Biology, Vol. 426, Issue 6 https://doi.org/10.1016/j.jmb.2013.10.033	journal	March 2014
Biochemical Characterization of APOBEC3H Variants: Implications for Their HIV-1 Restriction Activity and mC Modification Gu, Jiang; Chen, Qihan; Xiao, Xiao Journal of Molecular Biology, Vol. 428, Issue 23 https://doi.org/10.1016/j.jmb.2016.08.012	journal	November 2016
Molecular Interactions of a DNA Modifying Enzyme APOBEC3F Catalytic Domain with a Single-Stranded DNA Fang, Yao; Xiao, Xiao; Li, Shu-Xing Journal of Molecular Biology, Vol. 430, Issue 1 https://doi.org/10.1016/j.jmb.2017.11.007	journal	January 2018
AID Recognizes Structured DNA for Class Switch Recombination Qiao, Qi; Wang, Li; Meng, Fei-Long Molecular Cell, Vol. 67, Issue 3 https://doi.org/10.1016/j.molcel.2017.06.034	journal	August 2017
Crystal Structure of the DNA Cytosine Deaminase APOBEC3F: The Catalytically Active and HIV-1 Vif-Binding Domain Bohn, Markus-Frederik; Shandilya, Shivender M. D.; Albin, John S. Structure, Vol. 21, Issue 6 https://doi.org/10.1016/j.str.2013.04.010	journal	June 2013
Structure-Guided Analysis of the Human APOBEC3-HIV Restrictome Aydin, Halil; Taylor, Matthew W.; Lee, Jeffrey E. Structure, Vol. 22, Issue 5 https://doi.org/10.1016/j.str.2014.02.011	journal	May 2014
APOBECs and virus restriction Harris, Reuben S.; Dudley, Jaquelin P. Virology, Vol. 479-480 https://doi.org/10.1016/j.virol.2015.03.012	journal	May 2015
A Novel Cytidine Deaminase Affects Antibody Diversity Longacre, Angelika; Storb, Ursula Cell, Vol. 102, Issue 5 https://doi.org/10.1016/s0092-8674(00)00075-1	journal	September 2000
Cellular APOBEC3G restricts HIV-1 infection in resting CD4+ T cells Chiu, Ya-Lin; Soros, Vanessa B.; Kreisberg, Jason F. Nature, Vol. 435, Issue 7038 https://doi.org/10.1038/nature03493	journal	April 2005
Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implications Holden, Lauren G.; Prochnow, Courtney; Chang, Y. Paul Nature, Vol. 456, Issue 7218 https://doi.org/10.1038/nature07357	journal	October 2008
Reprogramming towards pluripotency requires AID-dependent DNA demethylation Bhutani, Nidhi; Brady, Jennifer J.; Damian, Mara Nature, Vol. 463, Issue 7284 https://doi.org/10.1038/nature08752	journal	December 2009
Genome-wide erasure of DNA methylation in mouse primordial germ cells is affected by AID deficiency Popp, Christian; Dean, Wendy; Feng, Suhua Nature, Vol. 463, Issue 7284 https://doi.org/10.1038/nature08829	journal	January 2010
AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes Kumar, Ritu; DiMenna, Lauren; Schrode, Nadine Nature, Vol. 500, Issue 7460 https://doi.org/10.1038/nature12299	journal	June 2013
Crystal structures of APOBEC3G N-domain alone and its complex with DNA Xiao, Xiao; Li, Shu-Xing; Yang, Hanjing Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms12193	journal	August 2016
The DNA cytosine deaminase APOBEC3H haplotype I likely contributes to breast and lung cancer mutagenesis Starrett, Gabriel J.; Luengas, Elizabeth M.; McCann, Jennifer L. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms12918	journal	September 2016
NMR structure of human restriction factor APOBEC3A reveals substrate binding and enzyme specificity Byeon, In-Ja L.; Ahn, Jinwoo; Mitra, Mithun Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms2883	journal	May 2013
Structural determinants of HIV-1 Vif susceptibility and DNA binding in APOBEC3F Siu, Karen K.; Sultana, Azmiri; Azimi, Farshad C. Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms3593	journal	November 2013
Evidence for APOBEC3B mutagenesis in multiple human cancers Burns, Michael B.; Temiz, Nuri A.; Harris, Reuben S. Nature Genetics, Vol. 45, Issue 9 https://doi.org/10.1038/ng.2701	journal	July 2013
APOBEC3B mutagenesis in cancer Kuong, Kawai J.; Loeb, Lawrence A. Nature Genetics, Vol. 45, Issue 9 https://doi.org/10.1038/ng.2736	journal	August 2013
The APOBEC3C crystal structure and the interface for HIV-1 Vif binding Kitamura, Shingo; Ode, Hirotaka; Nakashima, Masaaki Nature Structural & Molecular Biology, Vol. 19, Issue 10 https://doi.org/10.1038/nsmb.2378	journal	September 2012
APOBEC3G DNA deaminase acts processively 3′ → 5′ on single-stranded DNA Chelico, Linda; Pham, Phuong; Calabrese, Peter Nature Structural & Molecular Biology, Vol. 13, Issue 5 https://doi.org/10.1038/nsmb1086	journal	April 2006
APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA Bohn, Jennifer A.; Thummar, Keyur; York, Ashley Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01309-6	journal	October 2017
Deamination-independent restriction of LINE-1 retrotransposition by APOBEC3H Feng, Yuqing; Goubran, Mariam H.; Follack, Tyson B. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-11344-4	journal	September 2017
DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B Fu, Yang; Ito, Fumiaki; Zhang, Gewen Biochemical Journal, Vol. 471, Issue 1 https://doi.org/10.1042/bj20150382	journal	September 2015
Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase Bransteitter, R.; Pham, P.; Scharff, M. D. Proceedings of the National Academy of Sciences, Vol. 100, Issue 7 https://doi.org/10.1073/pnas.0730835100	journal	March 2003
Natural Polymorphisms and Oligomerization of Human APOBEC3H Contribute to Single-stranded DNA Scanning Ability Feng, Yuqing; Love, Robin P.; Ara, Anjuman Journal of Biological Chemistry, Vol. 290, Issue 45 https://doi.org/10.1074/jbc.m115.666065	journal	September 2015
Human Cytidine Deaminase APOBEC3H Restricts HIV-1 Replication Dang, Ying; Siew, Lai Mun; Wang, Xiaojun Journal of Biological Chemistry, Vol. 283, Issue 17 https://doi.org/10.1074/jbc.m707586200	journal	February 2008
APOBEC3 Cytidine Deaminases: Distinct Antiviral Actions along the Retroviral Life Cycle Chiu, Ya-Lin; Greene, Warner C. Journal of Biological Chemistry, Vol. 281, Issue 13 https://doi.org/10.1074/jbc.r500021200	journal	December 2005
Evolution of class switch recombination function in fish activation-induced cytidine deaminase, AID Wakae, Koshou; Magor, Brad G.; Saunders, Holly International Immunology, Vol. 18, Issue 1 https://doi.org/10.1093/intimm/dxh347	journal	November 2005
Evolution of the AID/APOBEC Family of Polynucleotide (Deoxy)cytidine Deaminases Conticello, Silvestro G.; Thomas, Cornelia J. F.; Petersen-Mahrt, Svend K. Molecular Biology and Evolution, Vol. 22, Issue 2 https://doi.org/10.1093/molbev/msi026	journal	October 2004
Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation Xiao, Xiao; Yang, Hanjing; Arutiunian, Vagan Nucleic Acids Research, Vol. 45, Issue 12 https://doi.org/10.1093/nar/gkx564	journal	June 2017
Sole copy of Z2-type human cytidine deaminase APOBEC3H has inhibitory activity against retrotransposons and HIV-1 Tan, Lindi; Sarkis, Phuong Thi Nguyen; Wang, Tao The FASEB Journal, Vol. 23, Issue 1 https://doi.org/10.1096/fj.07-088781	journal	January 2009
A critical role for AID in the initiation of reprogramming to induced pluripotent stem cells Bhutani, Nidhi; Decker, Matthew N.; Brady, Jennifer J. The FASEB Journal, Vol. 27, Issue 3 https://doi.org/10.1096/fj.12-222125	journal	March 2013
HIV Restriction Factors and Mechanisms of Evasion Malim, M. H.; Bieniasz, P. D. Cold Spring Harbor Perspectives in Medicine, Vol. 2, Issue 5 https://doi.org/10.1101/cshperspect.a006940	journal	February 2012
Polymorphism in Human APOBEC3H Affects a Phenotype Dominant for Subcellular Localization and Antiviral Activity Li, M. M. H.; Emerman, M. Journal of Virology, Vol. 85, Issue 16 https://doi.org/10.1128/jvi.00624-11	journal	June 2011
The Localization of APOBEC3H Variants in HIV-1 Virions Determines Their Antiviral Activity Ooms, M.; Majdak, S.; Seibert, C. W. Journal of Virology, Vol. 84, Issue 16 https://doi.org/10.1128/jvi.00754-10	journal	June 2010
Polymorphisms and Splice Variants Influence the Antiretroviral Activity of Human APOBEC3H Harari, A.; Ooms, M.; Mulder, L. C. F. Journal of Virology, Vol. 83, Issue 1 https://doi.org/10.1128/jvi.01665-08	journal	October 2008
Guidelines for Naming Nonprimate APOBEC3 Genes and Proteins LaRue, R. S.; Andresdottir, V.; Blanchard, Y. Journal of Virology, Vol. 83, Issue 2 https://doi.org/10.1128/jvi.01976-08	journal	November 2008
Analysis of Human APOBEC3H Haplotypes and Anti-Human Immunodeficiency Virus Type 1 Activity Wang, X.; Abudu, A.; Son, S. Journal of Virology, Vol. 85, Issue 7 https://doi.org/10.1128/jvi.02049-10	journal	January 2011
APOBEC Enzymes: Mutagenic Fuel for Cancer Evolution and Heterogeneity Swanton, C.; McGranahan, N.; Starrett, G. J. Cancer Discovery, Vol. 5, Issue 7 https://doi.org/10.1158/2159-8290.cd-15-0344	journal	June 2015
Expression of DNA Cytosine Deaminase APOBEC3 Proteins, a Potential Source for Producing Mutations, in Gastric, Colorectal and Prostate Cancers Gwak, Min; Choi, Youn Jin; Yoo, Nam Jin Tumori Journal, Vol. 100, Issue 4 https://doi.org/10.1177/1636.17922	journal	July 2014
The artiodactyl APOBEC3 innate immune repertoire shows evidence for a multi-functional domain organization that existed in the ancestor of placental mammals LaRue, Rebecca S.; Jónsson, Stefán R.; Silverstein, Kevin AT BMC Molecular Biology, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2199-9-104	journal	January 2008
The AID/APOBEC family of nucleic acid mutators Conticello, Silvestro G. Genome Biology, Vol. 9, Issue 6 https://doi.org/10.1186/gb-2008-9-6-229	journal	January 2008
Sequence and structural determinants of human APOBEC3H deaminase and anti-HIV-1 activities Mitra, Mithun; Singer, Dustin; Mano, Yu Retrovirology, Vol. 12, Issue 1 https://doi.org/10.1186/s12977-014-0130-8	journal	January 2015
Natural Polymorphisms in Human APOBEC3H and HIV-1 Vif Combine in Primary T Lymphocytes to Affect Viral G-to-A Mutation Levels and Infectivity Refsland, Eric W.; Hultquist, Judd F.; Luengas, Elizabeth M. PLoS Genetics, Vol. 10, Issue 11 https://doi.org/10.1371/journal.pgen.1004761	journal	November 2014
The Resistance of Human APOBEC3H to HIV-1 NL4-3 Molecular Clone Is Determined by a Single Amino Acid in Vif Ooms, Marcel; Letko, Michael; Binka, Mawuena PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0057744	journal	February 2013
The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid York, Ashley; Kutluay, Sebla B.; Errando, Manel PLOS Pathogens, Vol. 12, Issue 8 https://doi.org/10.1371/journal.ppat.1005833	journal	August 2016
Subcellular localization of the APOBEC3 proteins during mitosis and implications for genomic DNA deamination Lackey, Lela; Law, Emily K.; Brown, William L. Cell Cycle, Vol. 12, Issue 5 https://doi.org/10.4161/cc.23713	journal	March 2013
Activation-Induced Cytidine Deaminase (AID) Deficiency Causes the Autosomal Recessive Form of the Hyper-IgM Syndrome (HIGM2) Revy, Patrick; Muto, Taro; Levy, Yves Cell, Vol. 102, Issue 5 https://doi.org/10.1016/S0092-8674(00)00079-9	journal	September 2000
Antiretroelement Activity of APOBEC3H Was Lost Twice in Recent Human Evolution OhAinle, Molly; Kerns, Julie A.; Li, Melody M. H. Cell Host & Microbe, Vol. 4, Issue 3 https://doi.org/10.1016/j.chom.2008.07.005	journal	September 2008
Aid Honjo, Tasuku; Muramatsu, Masamichi; Fagarasan, Sidonia Immunity, Vol. 20, Issue 6 https://doi.org/10.1016/j.immuni.2004.05.011	journal	June 2004
Interaction with 7SL RNA but Not with HIV-1 Genomic RNA or P Bodies Is Required for APOBEC3F Virion Packaging Wang, Tao; Tian, Chunjuan; Zhang, Wenyan Journal of Molecular Biology, Vol. 375, Issue 4 https://doi.org/10.1016/j.jmb.2007.11.017	journal	January 2008
APOBEC3 Multimerization Correlates with HIV-1 Packaging and Restriction Activity in Living Cells Li, Jinhui; Chen, Yan; Li, Ming Journal of Molecular Biology, Vol. 426, Issue 6 https://doi.org/10.1016/j.jmb.2013.12.014	journal	March 2014
The in vitro Biochemical Characterization of an HIV-1 Restriction Factor APOBEC3F: Importance of Loop 7 on Both CD1 and CD2 for DNA Binding and Deamination Chen, Qihan; Xiao, Xiao; Wolfe, Aaron Journal of Molecular Biology, Vol. 428, Issue 13 https://doi.org/10.1016/j.jmb.2016.03.031	journal	July 2016
Mapping Region of Human Restriction Factor APOBEC3H Critical for Interaction with HIV-1 Vif Nakashima, Masaaki; Tsuzuki, Shinya; Awazu, Hiroaki Journal of Molecular Biology, Vol. 429, Issue 8 https://doi.org/10.1016/j.jmb.2017.03.019	journal	April 2017
Family-Wide Comparative Analysis of Cytidine and Methylcytidine Deamination by Eleven Human APOBEC Proteins Ito, Fumiaki; Fu, Yang; Kao, Shen-Chi A. Journal of Molecular Biology, Vol. 429, Issue 12 https://doi.org/10.1016/j.jmb.2017.04.021	journal	June 2017
The Antiviral and Cancer Genomic DNA Deaminase APOBEC3H Is Regulated by an RNA-Mediated Dimerization Mechanism Shaban, Nadine M.; Shi, Ke; Lauer, Kate V. Molecular Cell, Vol. 69, Issue 1 https://doi.org/10.1016/j.molcel.2017.12.010	journal	January 2018
Crystal Structure of the APOBEC3G Catalytic Domain Reveals Potential Oligomerization Interfaces Shandilya, Shivender M. D.; Nalam, Madhavi N. L.; Nalivaika, Ellen A. Structure, Vol. 18, Issue 1 https://doi.org/10.1016/j.str.2009.10.016	journal	January 2010
The ssDNA Mutator APOBEC3A Is Regulated by Cooperative Dimerization Bohn, Markus-Frederik; Shandilya, Shivender M. D.; Silvas, Tania V. Structure, Vol. 23, Issue 5 https://doi.org/10.1016/j.str.2015.03.016	journal	May 2015
The APOBEC-2 crystal structure and functional implications for the deaminase AID Prochnow, Courtney; Bransteitter, Ronda; Klein, Michael G. Nature, Vol. 445, Issue 7126 https://doi.org/10.1038/nature05492	journal	December 2006
AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation Nabel, Christopher S.; Jia, Huijue; Ye, Yu Nature Chemical Biology, Vol. 8, Issue 9 https://doi.org/10.1038/nchembio.1042	journal	July 2012
An APOBEC cytidine deaminase mutagenesis pattern is widespread in human cancers Roberts, Steven A.; Lawrence, Michael S.; Klimczak, Leszek J. Nature Genetics, Vol. 45, Issue 9 https://doi.org/10.1038/ng.2702	journal	July 2013
DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B Fu, Yang; Ito, Fumiaki; Zhang, Gewen Biochemical Journal, Vol. 471, Issue 1 https://doi.org/10.1042/BJ20150382	journal	September 2015
Methylcytosine and Normal Cytosine Deamination by the Foreign DNA Restriction Enzyme APOBEC3A Carpenter, Michael A.; Li, Ming; Rathore, Anurag Journal of Biological Chemistry, Vol. 287, Issue 41 https://doi.org/10.1074/jbc.M112.385161	journal	August 2012
Natural Polymorphisms and Oligomerization of Human APOBEC3H Contribute to Single-stranded DNA Scanning Ability Feng, Yuqing; Love, Robin P.; Ara, Anjuman Journal of Biological Chemistry, Vol. 290, Issue 45 https://doi.org/10.1074/jbc.M115.666065	journal	September 2015
Crystal Structure of the DNA Deaminase APOBEC3B Catalytic Domain Shi, Ke; Carpenter, Michael A.; Kurahashi, Kayo Journal of Biological Chemistry, Vol. 290, Issue 47 https://doi.org/10.1074/jbc.M115.679951	journal	September 2015
Human Cytidine Deaminase APOBEC3H Restricts HIV-1 Replication Dang, Ying; Siew, Lai Mun; Wang, Xiaojun Journal of Biological Chemistry, Vol. 283, Issue 17 https://doi.org/10.1074/jbc.M707586200	journal	February 2008
APOBEC3 Cytidine Deaminases: Distinct Antiviral Actions along the Retroviral Life Cycle Chiu, Ya-Lin; Greene, Warner C. Journal of Biological Chemistry, Vol. 281, Issue 13 https://doi.org/10.1074/jbc.R500021200	journal	December 2005
Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation Xiao, Xiao; Yang, Hanjing; Arutiunian, Vagan Nucleic Acids Research, Vol. 45, Issue 12 https://doi.org/10.1093/nar/gkx362	journal	May 2017
Hydrogen bonds are a primary driving force for de novo protein folding Lee, Schuyler; Wang, Chao; Liu, Haolin Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 12 https://doi.org/10.1107/S2059798317015303	journal	November 2017
Polymorphism in Human APOBEC3H Affects a Phenotype Dominant for Subcellular Localization and Antiviral Activity Li, M. M. H.; Emerman, M. Journal of Virology, Vol. 85, Issue 16 https://doi.org/10.1128/JVI.00624-11	journal	June 2011
The Localization of APOBEC3H Variants in HIV-1 Virions Determines Their Antiviral Activity Ooms, M.; Majdak, S.; Seibert, C. W. Journal of Virology, Vol. 84, Issue 16 https://doi.org/10.1128/JVI.00754-10	journal	June 2010
Polymorphisms and Splice Variants Influence the Antiretroviral Activity of Human APOBEC3H Harari, A.; Ooms, M.; Mulder, L. C. F. Journal of Virology, Vol. 83, Issue 1 https://doi.org/10.1128/JVI.01665-08	journal	October 2008
Guidelines for Naming Nonprimate APOBEC3 Genes and Proteins LaRue, R. S.; Andresdottir, V.; Blanchard, Y. Journal of Virology, Vol. 83, Issue 2 https://doi.org/10.1128/JVI.01976-08	journal	November 2008
Analysis of Human APOBEC3H Haplotypes and Anti-Human Immunodeficiency Virus Type 1 Activity Wang, X.; Abudu, A.; Son, S. Journal of Virology, Vol. 85, Issue 7 https://doi.org/10.1128/JVI.02049-10	journal	January 2011
Determinants of Efficient Degradation of APOBEC3 Restriction Factors by HIV-1 Vif Baig, T. T.; Feng, Y.; Chelico, L. Journal of Virology, Vol. 88, Issue 24 https://doi.org/10.1128/JVI.02484-14	journal	October 2014
Adaptive Evolution and Antiviral Activity of the Conserved Mammalian Cytidine Deaminase APOBEC3H OhAinle, M.; Kerns, J. A.; Malik, H. S. Journal of Virology, Vol. 80, Issue 8 https://doi.org/10.1128/JVI.80.8.3853-3862.2006	journal	March 2006
APOBEC Enzymes: Mutagenic Fuel for Cancer Evolution and Heterogeneity Swanton, C.; McGranahan, N.; Starrett, G. J. Cancer Discovery, Vol. 5, Issue 7 https://doi.org/10.1158/2159-8290.CD-15-0344	journal	June 2015
Reduced APOBEC3H Variant Anti-Viral Activities Are Associated with Altered RNA Binding Activities Zhen, Anjie; Du, Juan; Zhou, Xiaohong PLoS ONE, Vol. 7, Issue 7 https://doi.org/10.1371/journal.pone.0038771	journal	July 2012
Efficient Deamination of 5-Methylcytidine and 5-Substituted Cytidine Residues in DNA by Human APOBEC3A Cytidine Deaminase Suspène, Rodolphe; Aynaud, Marie-Ming; Vartanian, Jean-Pierre PLoS ONE, Vol. 8, Issue 6 https://doi.org/10.1371/journal.pone.0063461	journal	June 2013
Promiscuous RNA Binding Ensures Effective Encapsidation of APOBEC3 Proteins by HIV-1 Apolonia, Luis; Schulz, Reiner; Curk, Tomaž PLOS Pathogens, Vol. 11, Issue 1 https://doi.org/10.1371/journal.ppat.1004609	journal	January 2015
AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation Yi, Zhang,; Li, Shen,; Yu, Ye, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/1wtq-4483	text	January 2012
The Antiviral and Cancer Genomic DNA Deaminase APOBEC3H Is Regulated by an RNA-Mediated Dimerization Mechanism Shaban, Nadine Mendeley https://doi.org/10.17632/6mdtnv4kjb.1	dataset	January 2017

Cited By (5)

Structural basis of chimpanzee APOBEC3H dimerization stabilized by double-stranded RNA Matsuoka, Tatsuya; Nagae, Takayuki; Ode, Hirotaka Nucleic Acids Research, Vol. 46, Issue 19 https://doi.org/10.1093/nar/gky676	journal	July 2018
Structural perspectives on HIV‐1 Vif and APOBEC3 restriction factor interactions Azimi, Farshad C.; Lee, Jeffrey E. Protein Science, Vol. 29, Issue 2 https://doi.org/10.1002/pro.3729	journal	November 2019
Recurrent Loss of APOBEC3H Activity during Primate Evolution Garcia, Erin I.; Emerman, Michael Journal of Virology, Vol. 92, Issue 17 https://doi.org/10.1128/jvi.00971-18	journal	June 2018
Flexibility in Nucleic Acid Binding Is Central to APOBEC3H Antiviral Activity Bohn, Jennifer A.; DaSilva, Justin; Kharytonchyk, Siarhei Journal of Virology, Vol. 93, Issue 24 https://doi.org/10.1128/jvi.01275-19	journal	October 2019
APOBEC3H Subcellular Localization Determinants Define Zipcode for Targeting HIV-1 for Restriction Salamango, Daniel J.; Becker, Jordan T.; McCann, Jennifer L. Molecular and Cellular Biology, Vol. 38, Issue 23 https://doi.org/10.1128/mcb.00356-18	journal	September 2018

Similar Records

The Antiviral and Cancer Genomic DNA Deaminase APOBEC3H Is Regulated by an RNA-Mediated Dimerization Mechanism

Journal Article · Wed Dec 27 23:00:00 EST 2017 · Molecular Cell · OSTI ID:1432879

Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation

Journal Article · Tue May 30 00:00:00 EDT 2017 · Nucleic Acids Research · OSTI ID:1372228

APOBEC3B lysine residues are dispensable for DNA cytosine deamination, HIV-1 restriction, and nuclear localization

Journal Article · Tue Nov 14 23:00:00 EST 2017 · Virology · OSTI ID:22722972

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Metalloproteins
X-ray crystallography

Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H

Citation Formats

References (86)

Cited By (5)

Similar Records

Related Subjects