skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Novel Agents for Gram-Negative Biodefense Pathogens Final Report CRADA No. TC02128.0

; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
OSTI Identifier:
Report Number(s):
DOE Contract Number:
Resource Type:
Technical Report
Country of Publication:
United States
09-Biological and Medical Sciences; 50-Military Science

Citation Formats

Finn, J, Lightstone, F, and Turteltaub, K W. Novel Agents for Gram-Negative Biodefense Pathogens Final Report CRADA No. TC02128.0. United States: N. p., 2018. Web. doi:10.2172/1424632.
Finn, J, Lightstone, F, & Turteltaub, K W. Novel Agents for Gram-Negative Biodefense Pathogens Final Report CRADA No. TC02128.0. United States. doi:10.2172/1424632.
Finn, J, Lightstone, F, and Turteltaub, K W. 2018. "Novel Agents for Gram-Negative Biodefense Pathogens Final Report CRADA No. TC02128.0". United States. doi:10.2172/1424632.
title = {Novel Agents for Gram-Negative Biodefense Pathogens Final Report CRADA No. TC02128.0},
author = {Finn, J and Lightstone, F and Turteltaub, K W},
abstractNote = {},
doi = {10.2172/1424632},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2018,
month = 1

Technical Report:

Save / Share:
  • The effects of 2,4,6-trinitrotoluene (TNT) on indigenous soil populations and pure bacterial cultures were examined. The number of colony-forming units (CFU) appearing when TNT-contaminated soil was spread on 0.3% molasses plates decreased by 50% when the agar was amended with 67 {mu}g TNT mL{sup -1}, whereas a 99% reduction was observed when uncontaminated soil was plated. Furthermore, TNT-contaminated soil harbored a greater number of organisms able to grow on plates amended with greater than 10 {mu}g TNT mL{sup -1}. The percentage of gram-positive isolates was markedly less in TNT-contaminated soil (7%; 2 of 30) than in uncontaminated soil (61%; 20more » of 33). Pseudomonas aeruginosa, Pseudomonas corrugate, Pseudomonasfluorescens and Alcaligenes xylosoxidans made up the majority of the gram-negative isolates from TNT-contaminated soil. Gram-positive isolates from both soils demonstrated marked growth inhibition when greater than 8-16 {mu}g TNT mL{sup -1} was present in the culture media. Most pure cultures of known aerobic gram-negative organisms readily degraded TNT and evidenced net consumption of reduced metabolites. However, pure cultures of aerobic gram-positive bacteria were sensitive to relatively low concentrations of TNT as indicated by the 50% reduction in growth and TNT transformation which was observed at approximately 10 {mu}g TNT mL{sup -1}. Most non-sporeforming gram-positive organisms incubated in molasses media amended with 80 {mu}g TNT mL{sup -1} or greater became unculturable, whereas all strains tested remained culturable when incubated in mineral media amended with 98 {mu}g TNT mL{sup -1}, indicating that TNT sensitivity is likely linked to cell growth. These results indicate that gram-negative organisms are most likely responsible for any TNT transformation in contaminated soil, due to their relative insensitivity to high TNT concentrations and their ability to transform TNT.« less
  • The goal of this work was to use electrochemically driven pH control to develop a second generation, enzyme-based contained liquid membrane (CLM) permeator to extract CO{sub 2} from a variety of coal-based flue gas streams more efficiently than does the CLM current design, while achieving performance coincident with DOE targets of less than 45% Cost of electricity (COE) in 2007 and less than 20% COE in 2012. Central to this goal the CLM would be alkaline (>pH 8) at the feed gas side and acid (<pH 6) at the permeate side. Argonne demonstrated the technical feasibility for CO{sub 2} capturemore » and release using Argonne's resin-wafer electrode ionization (RW-EDI) system integrated with Carbozyme's carbonic anhydrase (CA) enzyme. Argonne developed RW-EDI for pH controlled desalination of process streams (e.g. Patents 7,452,920 & 7,306,934). In the current work, Argonne captured CO{sub 2} as HCO{sub 3}{sup -} and released it as CO{sub 2}. The goal is to both capture CO{sub 2} from a simulated flue gas stream and release it within the DOE targets for increase in COE. Initial performance results indicate that the 2012 COE targets are achievable with the developed technology. The design is subject to patent-hold. This task was funded in an exploratory phase, so no process optimization was attempted. Argonne believes that with optimization this performance could be significantly improved.« less
  • Evaluation of novel semiconductor materials potentially useful in solar cells. NREL will fabricate, test and analyze solar cells from EpiWorks' wafers produced in 2-3 separate growth campaigns. NREL will also characterize material from 2-3 separate EpiWorks material development campaigns. Finally, NREL will visit EpiWorks and help establish any necessary process, such as spectral CV measurements and III-V on Si metalization processes and help validate solar cell designs and performance.