Droplet microfluidics for synthetic biology

Gach, Philip Charles; Iwai, Kosuke; Kim, Peter Wonhee; Hillson, Nathan J.; Singh, Anup K.

doi:10.1039/C7LC00576H

Droplet microfluidics for synthetic biology

Journal Article · Thu Aug 10 00:00:00 EDT 2017 · Lab on a chip (Print)

DOI:https://doi.org/10.1039/C7LC00576H· OSTI ID:1421856

Gach, Philip Charles ^[1]; Iwai, Kosuke ^[1]; Kim, Peter Wonhee ^[1]; Hillson, Nathan J. ^[2]; ^[1]

DOE Joint BioEnergy Institute, Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
DOE Joint BioEnergy Institute, Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Here, synthetic biology is an interdisciplinary field that aims to engineer biological systems for useful purposes. Organism engineering often requires the optimization of individual genes and/or entire biological pathways (consisting of multiple genes). Advances in DNA sequencing and synthesis have recently begun to enable the possibility of evaluating thousands of gene variants and hundreds of thousands of gene combinations. However, such large-scale optimization experiments remain cost-prohibitive to researchers following traditional molecular biology practices, which are frequently labor-intensive and suffer from poor reproducibility. Liquid handling robotics may reduce labor and improve reproducibility, but are themselves expensive and thus inaccessible to most researchers. Microfluidic platforms offer a lower entry price point alternative to robotics, and maintain high throughput and reproducibility while further reducing operating costs through diminished reagent volume requirements. Droplet microfluidics have shown exceptional promise for synthetic biology experiments, including DNA assembly, transformation/transfection, culturing, cell sorting, phenotypic assays, artificial cells and genetic circuits.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1421856

Report Number(s):: SAND--2017-9052J; 656492

Journal Information:: Lab on a chip (Print), Journal Name: Lab on a chip (Print) Journal Issue: 20 Vol. 17; ISSN LCAHAM; ISSN 1473-0197

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

References (126)

On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics Gasilova, Natalia; Yu, Qiuliyang; Qiao, Liang Angewandte Chemie, Vol. 126, Issue 17 https://doi.org/10.1002/ange.201310795	journal	March 2014
On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics Gasilova, Natalia; Yu, Qiuliyang; Qiao, Liang Angewandte Chemie International Edition, Vol. 53, Issue 17 https://doi.org/10.1002/anie.201310795	journal	March 2014
Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water van Swaay, Dirk; Tang, T. -Y. Dora; Mann, Stephen Angewandte Chemie International Edition, Vol. 54, Issue 29 https://doi.org/10.1002/anie.201502886	journal	May 2015
Electroporation of micro-droplet encapsulated HeLa cells in oil phase Xiao, Kang; Zhang, Mengying; Chen, Shuyu ELECTROPHORESIS, Vol. 31, Issue 18 https://doi.org/10.1002/elps.201000155	journal	August 2010
Lipid Bilayers on a Picoliter Microdroplet Array for Rapid Fluorescence Detection of Membrane Transport Tonooka, Taishi; Sato, Koji; Osaki, Toshihisa Small, Vol. 10, Issue 16 https://doi.org/10.1002/smll.201303332	journal	March 2014
Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays Zhu, Ying; Warrick, Jay W.; Haubert, Kathryn Biomedical Microdevices, Vol. 11, Issue 3 https://doi.org/10.1007/s10544-008-9263-7	journal	January 2009
Integrated microbioreactor for culture and analysis of bacteria, algae and yeast Au, Sam H.; Shih, Steve C. C.; Wheeler, Aaron R. Biomedical Microdevices, Vol. 13, Issue 1 https://doi.org/10.1007/s10544-010-9469-3	journal	September 2010
Microdroplet-based cell culture models and their application Jang, Minjeong; Yang, Seungwon; Kim, Pilnam BioChip Journal, Vol. 10, Issue 4 https://doi.org/10.1007/s13206-016-0407-1	journal	November 2016
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis Beaucage, S. L.; Caruthers, M. H. Tetrahedron Letters, Vol. 22, Issue 20, p. 1859-1862 https://doi.org/10.1016/S0040-4039(01)90461-7	journal	January 1981
Comparison of plasma sample purification by manual liquid–liquid extraction, automated 96-well liquid–liquid extraction and automated 96-well solid-phase extraction for analysis by high-performance liquid chromatography with tandem mass spectrometry Jemal, Mohammed; Teitz, Deborah; Ouyang, Zheng Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 732, Issue 2 https://doi.org/10.1016/S0378-4347(99)00323-0	journal	September 1999
Transfection of Jurkat T cells by droplet electroporation Im, Do Jin; Jeong, Su-Nam Biochemical Engineering Journal, Vol. 122 https://doi.org/10.1016/j.bej.2017.03.010	journal	June 2017
Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells Klein, Allon M.; Mazutis, Linas; Akartuna, Ilke Cell, Vol. 161, Issue 5 https://doi.org/10.1016/j.cell.2015.04.044	journal	May 2015
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets Macosko, Evan Z.; Basu, Anindita; Satija, Rahul Cell, Vol. 161, Issue 5 https://doi.org/10.1016/j.cell.2015.05.002	journal	May 2015
Quorum-Sensing Crosstalk-Driven Synthetic Circuits: From Unimodality to Trimodality Wu, Fuqing; Menn, David J.; Wang, Xiao Chemistry & Biology, Vol. 21, Issue 12 https://doi.org/10.1016/j.chembiol.2014.10.008	journal	December 2014
Microfluidics-to-mass spectrometry: A review of coupling methods and applications Wang, Xue; Yi, Lian; Mukhitov, Nikita Journal of Chromatography A, Vol. 1382 https://doi.org/10.1016/j.chroma.2014.10.039	journal	February 2015
Capillary-composited microfluidic device for heat shock transformation of Escherichia coli Sha, Jun; Wang, Yaolei; Wang, Jianchun Journal of Bioscience and Bioengineering, Vol. 112, Issue 4 https://doi.org/10.1016/j.jbiosc.2011.06.004	journal	October 2011
Design and construction of small perturbation mutagenesis libraries for antibody affinity maturation using massive microchip-synthesized oligonucleotides Xu, Man; Hu, Siyi; Ding, Bo Journal of Biotechnology, Vol. 194 https://doi.org/10.1016/j.jbiotec.2014.11.007	journal	January 2015
Recent advances in microchip-mass spectrometry for biological analysis He, Xiangwei; Chen, Qiushui; Zhang, Yandong TrAC Trends in Analytical Chemistry, Vol. 53 https://doi.org/10.1016/j.trac.2013.09.013	journal	January 2014
Biochemical analysis on microfluidic chips Wu, Jing; He, Ziyi; Chen, Qiushui TrAC Trends in Analytical Chemistry, Vol. 80 https://doi.org/10.1016/j.trac.2016.03.013	journal	June 2016
Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets He, Mingyan; Edgar, J. Scott; Jeffries, Gavin D. M. Analytical Chemistry, Vol. 77, Issue 6 https://doi.org/10.1021/ac0480850	journal	March 2005
Automated Acoustic Matrix Deposition for MALDI Sample Preparation Aerni, Hans-Rudolf; Cornett, Dale S.; Caprioli, Richard M. Analytical Chemistry, Vol. 78, Issue 3 https://doi.org/10.1021/ac051534r	journal	February 2006
Chemical Transfection of Cells in Picoliter Aqueous Droplets in Fluorocarbon Oil Chen, Fangyuan; Zhan, Yihong; Geng, Tao Analytical Chemistry, Vol. 83, Issue 22 https://doi.org/10.1021/ac2022794	journal	November 2011
Interfacing Droplet Microfluidics with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Label-Free Content Analysis of Single Droplets Küster, Simon K.; Fagerer, Stephan R.; Verboket, Pascal E. Analytical Chemistry, Vol. 85, Issue 3 https://doi.org/10.1021/ac3033189	journal	January 2013
Sensitive, High Throughput Detection of Proteins in Individual, Surfactant-Stabilized Picoliter Droplets Using Nanoelectrospray Ionization Mass Spectrometry Smith, Clive A.; Li, Xin; Mize, Todd H. Analytical Chemistry, Vol. 85, Issue 8 https://doi.org/10.1021/ac400453t	journal	March 2013
Electroporation of Cells in Microfluidic Droplets Zhan, Yihong; Wang, Jun; Bao, Ning Analytical Chemistry, Vol. 81, Issue 5 https://doi.org/10.1021/ac9001172	journal	March 2009
Digital Microfluidic Approach for Efficient Electroporation with High Productivity: Transgene Expression of Microalgae without Cell Wall Removal Im, Do Jin; Jeong, Su-Nam; Yoo, Byeong Sun Analytical Chemistry, Vol. 87, Issue 13 https://doi.org/10.1021/acs.analchem.5b00725	journal	June 2015
Integrated Microfluidic System for Size-Based Selection and Trapping of Giant Vesicles Kazayama, Yuki; Teshima, Tetsuhiko; Osaki, Toshihisa Analytical Chemistry, Vol. 88, Issue 2 https://doi.org/10.1021/acs.analchem.5b03772	journal	December 2015
Specific and Reversible DNA-Directed Self-Assembly of Modular Vesicle-Droplet Hybrid Materials Hadorn, Maik; Boenzli, Eva; Hanczyc, Martin M. Langmuir, Vol. 32, Issue 15 https://doi.org/10.1021/acs.langmuir.5b04003	journal	March 2016
Microfluidic Fabrication of Pluronic Vesicles with Controlled Permeability do Nascimento, Débora F.; Arriaga, Laura R.; Eggersdorfer, Max Langmuir, Vol. 32, Issue 21 https://doi.org/10.1021/acs.langmuir.6b01399	journal	May 2016
Partitioning Variability of a Compartmentalized In Vitro Transcriptional Thresholding Circuit Kapsner, Korbinian; Simmel, Friedrich C. ACS Synthetic Biology, Vol. 4, Issue 10 https://doi.org/10.1021/acssynbio.5b00051	journal	May 2015
A Versatile Microfluidic Device for Automating Synthetic Biology Shih, Steve C. C.; Goyal, Garima; Kim, Peter W. ACS Synthetic Biology, Vol. 4, Issue 10 https://doi.org/10.1021/acssynbio.5b00062	journal	June 2015
Marching along to an Offbeat Drum: Entrainment of Synthetic Gene Oscillators by a Noisy Stimulus Butzin, Nicholas C.; Hochendoner, Philip; Ogle, Curtis T. ACS Synthetic Biology, Vol. 5, Issue 2 https://doi.org/10.1021/acssynbio.5b00127	journal	November 2015
Entrainment and Control of Bacterial Populations: An in Silico Study over a Spatially Extended Agent Based Model Mina, Petros; Tsaneva-Atanasova, Krasimira; Bernardo, Mario di ACS Synthetic Biology, Vol. 5, Issue 7 https://doi.org/10.1021/acssynbio.5b00243	journal	April 2016
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology Garamella, Jonathan; Marshall, Ryan; Rustad, Mark ACS Synthetic Biology, Vol. 5, Issue 4 https://doi.org/10.1021/acssynbio.5b00296	journal	February 2016
A Droplet Microfluidic Platform for Automating Genetic Engineering Gach, Philip C.; Shih, Steve C. C.; Sustarich, Jess ACS Synthetic Biology, Vol. 5, Issue 5 https://doi.org/10.1021/acssynbio.6b00011	journal	February 2016
Resource Sharing Controls Gene Expression Bursting Caveney, Patrick M.; Norred, S. Elizabeth; Chin, Charles W. ACS Synthetic Biology, Vol. 6, Issue 2 https://doi.org/10.1021/acssynbio.6b00189	journal	September 2016
Streamlining the Design-to-Build Transition with Build-Optimization Software Tools Oberortner, Ernst; Cheng, Jan-Fang; Hillson, Nathan J. ACS Synthetic Biology, Vol. 6, Issue 3 https://doi.org/10.1021/acssynbio.6b00200	journal	November 2016
Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform Madison, Andrew C.; Royal, Matthew W.; Vigneault, Frederic ACS Synthetic Biology, Vol. 6, Issue 9 https://doi.org/10.1021/acssynbio.7b00007	journal	June 2017
Fluorinated Pickering Emulsions Impede Interfacial Transport and Form Rigid Interface for the Growth of Anchorage-Dependent Cells Pan, Ming; Rosenfeld, Liat; Kim, Minkyu ACS Applied Materials & Interfaces, Vol. 6, Issue 23 https://doi.org/10.1021/am506443e	journal	November 2014
Communication and Computation by Bacteria Compartmentalized within Microemulsion Droplets Weitz, Maximilian; Mückl, Andrea; Kapsner, Korbinian Journal of the American Chemical Society, Vol. 136, Issue 1 https://doi.org/10.1021/ja411132w	journal	December 2013
j5 DNA Assembly Design Automation Software Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D. ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21 https://doi.org/10.1021/sb2000116	journal	December 2011
Accurate multiplex gene synthesis from programmable DNA microchips Tian, Jingdong; Gong, Hui; Sheng, Nijing Nature, Vol. 432, Issue 7020, p. 1050-1054 https://doi.org/10.1038/nature03151	journal	December 2004
A synchronized quorum of genetic clocks Danino, Tal; Mondragón-Palomino, Octavio; Tsimring, Lev Nature, Vol. 463, Issue 7279 https://doi.org/10.1038/nature08753	journal	January 2010
Rapid and tunable post-translational coupling of genetic circuits Prindle, Arthur; Selimkhanov, Jangir; Li, Howard Nature, Vol. 508, Issue 7496 https://doi.org/10.1038/nature13238	journal	April 2014
Synchronized cycles of bacterial lysis for in vivo delivery Din, M. Omar; Danino, Tal; Prindle, Arthur Nature, Vol. 536, Issue 7614 https://doi.org/10.1038/nature18930	journal	July 2016
Synchronous long-term oscillations in a synthetic gene circuit Potvin-Trottier, Laurent; Lord, Nathan D.; Vinnicombe, Glenn Nature, Vol. 538, Issue 7626 https://doi.org/10.1038/nature19841	journal	October 2016
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips Kosuri, Sriram; Eroshenko, Nikolai; LeProust, Emily M. Nature Biotechnology, Vol. 28, Issue 12, p. 1295-1299 https://doi.org/10.1038/nbt.1716	journal	November 2010
Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption Wang, Benjamin L.; Ghaderi, Adel; Zhou, Hang Nature Biotechnology, Vol. 32, Issue 5 https://doi.org/10.1038/nbt.2857	journal	April 2014
Digital-to-biological converter for on-demand production of biologics Boles, Kent S.; Kannan, Krishna; Gill, John Nature Biotechnology, Vol. 35, Issue 7 https://doi.org/10.1038/nbt.3859	journal	May 2017
Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding Lan, Freeman; Demaree, Benjamin; Ahmed, Noorsher Nature Biotechnology, Vol. 35, Issue 7 https://doi.org/10.1038/nbt.3880	journal	May 2017
Diversity in the dynamical behaviour of a compartmentalized programmable biochemical oscillator Weitz, Maximilian; Kim, Jongmin; Kapsner, Korbinian Nature Chemistry, Vol. 6, Issue 4 https://doi.org/10.1038/nchem.1869	journal	February 2014
High-resolution mapping of bifurcations in nonlinear biochemical circuits Genot, A. J.; Baccouche, A.; Sieskind, R. Nature Chemistry, Vol. 8, Issue 8 https://doi.org/10.1038/nchem.2544	journal	June 2016
Pulse-density modulation control of chemical oscillation far from equilibrium in a droplet open-reactor system Sugiura, Haruka; Ito, Manami; Okuaki, Tomoya Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms10212	journal	January 2016
A general strategy for expanding polymerase function by droplet microfluidics Larsen, Andrew C.; Dunn, Matthew R.; Hatch, Andrew Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms11235	journal	April 2016
High-throughput and combinatorial gene expression on a chip for metabolism-induced toxicology screening Kwon, Seok Joon; Lee, Dong Woo; Shah, Dhiral A. Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4739	journal	May 2014
Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways Elani, Yuval; Law, Robert V.; Ces, Oscar Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms6305	journal	October 2014
Enzymatic assembly of DNA molecules up to several hundred kilobases Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan Nature Methods, Vol. 6, Issue 5, p. 343-345 https://doi.org/10.1038/nmeth.1318	journal	April 2009
Large-scale de novo DNA synthesis: technologies and applications Kosuri, Sriram; Church, George M. Nature Methods, Vol. 11, Issue 5 https://doi.org/10.1038/nmeth.2918	journal	April 2014
Principles of genetic circuit design Brophy, Jennifer A. N.; Voigt, Christopher A. Nature Methods, Vol. 11, Issue 5 https://doi.org/10.1038/nmeth.2926	journal	April 2014
Single-cell analysis and sorting using droplet-based microfluidics Mazutis, Linas; Gilbert, John; Ung, W. Lloyd Nature Protocols, Vol. 8, Issue 5 https://doi.org/10.1038/nprot.2013.046	journal	April 2013
Synthetic biology: applications come of age Khalil, Ahmad S.; Collins, James J. Nature Reviews Genetics, Vol. 11, Issue 5 https://doi.org/10.1038/nrg2775	journal	May 2010
Microfluidic Preparation of Polymer-Nucleic Acid Nanocomplexes Improves Nonviral Gene Transfer Grigsby, Christopher L.; Ho, Yi-Ping; Lin, Chao Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep03155	journal	November 2013
Droplet-interface-bilayer assays in microfluidic passive networks Schlicht, Bárbara; Zagnoni, Michele Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep09951	journal	April 2015
Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters Soga, Naoki; Watanabe, Rikiya; Noji, Hiroyuki Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep11025	journal	June 2015
Detection of Infertility-related Neutralizing Antibodies with a Cell-free Microfluidic Method Eyer, Klaus; Root, Katharina; Verboket, Pascal E. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep16551	journal	November 2015
High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics Beneyton, Thomas; Wijaya, I. Putu Mahendra; Postros, Prexilia Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep27223	journal	June 2016
Label-free isolation and deposition of single bacterial cells from heterogeneous samples for clonal culturing Riba, J.; Gleichmann, T.; Zimmermann, S. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep32837	journal	September 2016
Mechanically activated artificial cell by using microfluidics Ho, Kenneth K. Y.; Lee, Lap Man; Liu, Allen P. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep32912	journal	September 2016
Sequence specific sorting of DNA molecules with FACS using 3dPCR Sukovich, David J.; Lance, Shea T.; Abate, Adam R. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep39385	journal	January 2017
High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells Bian, Shengtai; Zhou, Yicen; Hu, Yawei Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep42512	journal	February 2017
Cell-like liposomes integrated with microfluidic technology for synthetic biology Osaki, Toshihisa; Kamiya, Koki; Takeuchi, Shoji Synthetic Biology https://doi.org/10.1039/9781849737845-00275	book	January 2014
A microfluidic bioreactor for increased active retrovirus output Vu, Halong N.; Li, Yawen; Casali, Monica Lab Chip, Vol. 8, Issue 1 https://doi.org/10.1039/B711577F	journal	January 2008
Surfactants in droplet-based microfluidics Baret, Jean-Christophe Lab Chip, Vol. 12, Issue 3 https://doi.org/10.1039/C1LC20582J	journal	January 2012
Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors Elani, Yuval; Law, Robert V.; Ces, Oscar Physical Chemistry Chemical Physics, Vol. 17, Issue 24 https://doi.org/10.1039/C4CP05933F	journal	January 2015
Integration of microfluidics into the synthetic biology design flow Huang, Haiyao; Densmore, Douglas Lab Chip, Vol. 14, Issue 18 https://doi.org/10.1039/C4LC00509K	journal	January 2014
A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings Czekalska, Magdalena A.; Kaminski, Tomasz S.; Jakiela, Slawomir Lab on a Chip, Vol. 15, Issue 2 https://doi.org/10.1039/C4LC00985A	journal	January 2015
Rapid modulation of droplet composition with pincer microvalves Ochs, Christopher J.; Abate, Adam R. Lab on a Chip, Vol. 15, Issue 1 https://doi.org/10.1039/C4LC01040J	journal	January 2015
Accurate microfluidic sorting of droplets at 30 kHz Sciambi, Adam; Abate, Adam R. Lab on a Chip, Vol. 15, Issue 1 https://doi.org/10.1039/C4LC01194E	journal	January 2015
Trapping and release of giant unilamellar vesicles in microfluidic wells Yamada, Ayako; Lee, Sungyon; Bassereau, Patricia Soft Matter, Vol. 10, Issue 32 https://doi.org/10.1039/C4SM00065J	journal	January 2014
Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets Schwarz-Schilling, M.; Aufinger, L.; Mückl, A. Integrative Biology, Vol. 8, Issue 4 https://doi.org/10.1039/C5IB00301F	journal	January 2016
Continuous microfluidic fabrication of synthetic asymmetric vesicles Lu, Li; Schertzer, Jeffrey W.; Chiarot, Paul R. Lab on a Chip, Vol. 15, Issue 17 https://doi.org/10.1039/C5LC00520E	journal	January 2015
The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation Collins, David J.; Neild, Adrian; deMello, Andrew Lab on a Chip, Vol. 15, Issue 17 https://doi.org/10.1039/C5LC00614G	journal	January 2015
Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes Caschera, Filippo; Lee, Jin Woo; Ho, Kenneth K. Y. Chemical Communications, Vol. 52, Issue 31 https://doi.org/10.1039/C6CC00223D	journal	January 2016
On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening Heinemann, Joshua; Deng, Kai; Shih, Steve C. C. Lab on a Chip, Vol. 17, Issue 2 https://doi.org/10.1039/C6LC01182A	journal	January 2017
Cell-sized mechanosensitive and biosensing compartment programmed with DNA Majumder, Sagardip; Garamella, Jonathan; Wang, Ying-Lin Chemical Communications, Vol. 53, Issue 53 https://doi.org/10.1039/C7CC03455E	journal	January 2017
Dielectrowetting manipulation for digital microfluidics: creating, transporting, splitting, and merging of droplets Geng, Hongyao; Feng, Jian; Stabryla, Lisa Marie Lab on a Chip, Vol. 17, Issue 6 https://doi.org/10.1039/C7LC00006E	journal	January 2017
Droplet microfluidics Teh, Shia-Yen; Lin, Robert; Hung, Lung-Hsin Lab on a Chip, Vol. 8, Issue 2, p. 198-220 https://doi.org/10.1039/b715524g	journal	January 2008
Microfluidic based single cell microinjection Adamo, Andrea; Jensen, Klavs F. Lab on a Chip, Vol. 8, Issue 8 https://doi.org/10.1039/b803212b	journal	January 2008
Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity Baret, Jean-Christophe; Miller, Oliver J.; Taly, Valerie Lab on a Chip, Vol. 9, Issue 13, p. 1850-1858 https://doi.org/10.1039/b902504a	journal	January 2009
Heat-shock transformation of Escherichia coli in nanolitre droplets formed in a capillary-composited microfluidic device Sha, Jun; Wang, Yaolei; Wang, Jianchun Analytical Methods, Vol. 3, Issue 9 https://doi.org/10.1039/c1ay05189j	journal	January 2011
Microfluidic approaches for gene delivery and gene therapy Kim, Jungkyu; Hwang, Inseong; Britain, Derek Lab on a Chip, Vol. 11, Issue 23 https://doi.org/10.1039/c1lc20766k	journal	January 2011
Droplet microfluidics for high-throughput biological assays Guo, Mira T.; Rotem, Assaf; Heyman, John A. Lab on a Chip, Vol. 12, Issue 12 https://doi.org/10.1039/c2lc21147e	journal	January 2012
Digital microfluidics: a versatile tool for applications in chemistry, biology and medicine Jebrail, Mais J.; Bartsch, Michael S.; Patel, Kamlesh D. Lab on a Chip, Vol. 12, Issue 14 https://doi.org/10.1039/c2lc40318h	journal	January 2012
Electrode-free picoinjection of microfluidic drops O'Donovan, Brian; Eastburn, Dennis J.; Abate, Adam R. Lab on a Chip, Vol. 12, Issue 20 https://doi.org/10.1039/c2lc40693d	journal	January 2012
Microfluidic electro-sonoporation: a multi-modal cell poration methodology through simultaneous application of electric field and ultrasonic wave Longsine-Parker, Whitney; Wang, Han; Koo, Chiwan Lab on a Chip, Vol. 13, Issue 11 https://doi.org/10.1039/c3lc40877a	journal	January 2013
Building droplet-based microfluidic systems for biological analysis Niu, Xize; deMello, Andrew J. Biochemical Society Transactions, Vol. 40, Issue 4 https://doi.org/10.1042/BST20120005	journal	July 2012
Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology Elani, Yuval Biochemical Society Transactions, Vol. 44, Issue 3 https://doi.org/10.1042/BST20160052	journal	June 2016
DNA-library assembly programmed by on-demand nano-liter droplets from a custom microfluidic chip Tangen, Uwe; Minero, Gabriel Antonio S.; Sharma, Abhishek Biomicrofluidics, Vol. 9, Issue 4 https://doi.org/10.1063/1.4926616	journal	July 2015
Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads Moore, J. A.; Nemat-Gorgani, M.; Madison, A. C. Biomicrofluidics, Vol. 11, Issue 1 https://doi.org/10.1063/1.4975391	journal	January 2017
A vesicle bioreactor as a step toward an artificial cell assembly Noireaux, V.; Libchaber, A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 51 https://doi.org/10.1073/pnas.0408236101	journal	December 2004
Unilamellar vesicle formation and encapsulation by microfluidic jetting Stachowiak, J. C.; Richmond, D. L.; Li, T. H. Proceedings of the National Academy of Sciences, Vol. 105, Issue 12 https://doi.org/10.1073/pnas.0710875105	journal	March 2008
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution Agresti, J. J.; Antipov, E.; Abate, A. R. Proceedings of the National Academy of Sciences, Vol. 107, Issue 9, p. 4004-4009 https://doi.org/10.1073/pnas.0910781107	journal	February 2010
High-throughput injection with microfluidics using picoinjectors Abate, A. R.; Hung, T.; Mary, P. Proceedings of the National Academy of Sciences, Vol. 107, Issue 45 https://doi.org/10.1073/pnas.1006888107	journal	October 2010
Engineered temperature compensation in a synthetic genetic clock Hussain, F.; Gupta, C.; Hirning, A. J. Proceedings of the National Academy of Sciences, Vol. 111, Issue 3 https://doi.org/10.1073/pnas.1316298111	journal	January 2014
Dissecting enzyme function with microfluidic-based deep mutational scanning Romero, Philip A.; Tran, Tuan M.; Abate, Adam R. Proceedings of the National Academy of Sciences, Vol. 112, Issue 23 https://doi.org/10.1073/pnas.1422285112	journal	May 2015
Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast Huang, Mingtao; Bai, Yunpeng; Sjostrom, Staffan L. Proceedings of the National Academy of Sciences, Vol. 112, Issue 34 https://doi.org/10.1073/pnas.1506460112	journal	August 2015
Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity Terekhov, Stanislav S.; Smirnov, Ivan V.; Stepanova, Anastasiya V. Proceedings of the National Academy of Sciences, Vol. 114, Issue 10 https://doi.org/10.1073/pnas.1621226114	journal	February 2017
Principles of cell-free genetic circuit assembly Noireaux, V.; Bar-Ziv, R.; Libchaber, A. Proceedings of the National Academy of Sciences, Vol. 100, Issue 22 https://doi.org/10.1073/pnas.2135496100	journal	October 2003
Parallel gene synthesis in a microfluidic device Kong, D. S.; Carr, P. A.; Chen, L. Nucleic Acids Research, Vol. 35, Issue 8 https://doi.org/10.1093/nar/gkm121	journal	March 2007
A microfluidic oligonucleotide synthesizer Lee, Cheng-Chung; Snyder, Thomas M.; Quake, Stephen R. Nucleic Acids Research, Vol. 38, Issue 8 https://doi.org/10.1093/nar/gkq092	journal	February 2010
Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process LeProust, Emily M.; Peck, Bill J.; Spirin, Konstantin Nucleic Acids Research, Vol. 38, Issue 8 https://doi.org/10.1093/nar/gkq163	journal	March 2010
Synthesis and cell-free cloning of DNA libraries using programmable microfluidics Yehezkel, Tuval Ben; Rival, Arnaud; Raz, Ofir Nucleic Acids Research, Vol. 44, Issue 4 https://doi.org/10.1093/nar/gkv1087	journal	October 2015
Droplet-based microfluidics for artificial cell generation: a brief review Martino, Chiara; deMello, Andrew J. Interface Focus, Vol. 6, Issue 4 https://doi.org/10.1098/rsfs.2016.0011	journal	August 2016
Fluid Transport in Partially Shielded Electrowetting on Dielectric Digital Microfluidic Devices Madison, Andrew C.; Royal, Matthew W.; Fair, Richard B. Journal of Microelectromechanical Systems, Vol. 25, Issue 4 https://doi.org/10.1109/JMEMS.2016.2561699	journal	August 2016
CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation Han, Xin; Liu, Zongbin; Jo, Myeong chan Science Advances, Vol. 1, Issue 7 https://doi.org/10.1126/sciadv.1500454	journal	August 2015
Emergent genetic oscillations in a synthetic microbial consortium Chen, Y.; Kim, J. K.; Hirning, A. J. Science, Vol. 349, Issue 6251 https://doi.org/10.1126/science.aaa3794	journal	August 2015
High-Throughput Single-Cell Cultivation on Microfluidic Streak Plates Jiang, Cheng-Ying; Dong, Libing; Zhao, Jian-Kang Applied and Environmental Microbiology, Vol. 82, Issue 7 https://doi.org/10.1128/AEM.03588-15	journal	February 2016
Vesicles-on-a-chip: A universal microfluidic platform for the assembly of liposomes and polymersomes Petit, Julien; Polenz, Ingmar; Baret, Jean-Christophe The European Physical Journal E, Vol. 39, Issue 6 https://doi.org/10.1140/epje/i2016-16059-8	journal	June 2016
Digital Microfluidic Cell Culture Ng, Alphonsus H. C.; Li, Bingyu Betty; Chamberlain, M. Dean Annual Review of Biomedical Engineering, Vol. 17, Issue 1 https://doi.org/10.1146/annurev-bioeng-071114-040808	journal	December 2015
Artificial cell mimics as simplified models for the study of cell biology Salehi-Reyhani, Ali; Ces, Oscar; Elani, Yuval Experimental Biology and Medicine, Vol. 242, Issue 13 https://doi.org/10.1177/1535370217711441	journal	June 2017
A One Pot, One Step, Precision Cloning Method with High Throughput Capability Engler, Carola; Kandzia, Romy; Marillonnet, Sylvestre PLoS ONE, Vol. 3, Issue 11, Article No. e3647 https://doi.org/10.1371/journal.pone.0003647	journal	November 2008
Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies Hu, Dongmei; Hu, Siyi; Wan, Wen PLOS ONE, Vol. 10, Issue 6 https://doi.org/10.1371/journal.pone.0129125	journal	June 2015
Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encapsulated mammalian cell-free expression Ho, Kenneth K. Y.; Lee, Jin Woo; Durand, Grégory PLOS ONE, Vol. 12, Issue 3 https://doi.org/10.1371/journal.pone.0174689	journal	March 2017
Synchronous long-term oscillations in a synthetic gene circuit Potvin-Trottier, Laurent; Lord, Nathan D.; Vinnicombe, Glenn Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.956	text	January 2016
Microfluidic Production of Multiple Emulsions Vladisavljević, Goran; Al Nuumani, Ruqaya; Nabavi, Seyed Micromachines, Vol. 8, Issue 3 https://doi.org/10.3390/mi8030075	journal	March 2017
Detection of Infertility-related Neutralizing Antibodies with a Cell-free Microfluidic Method Eyer, Klaus; Root, Katharina; Verboket, Pascal E. ETH Zurich https://doi.org/10.3929/ethz-b-000107487	text	January 2015

Cited By (23)

Mastering Complexity: Towards Bottom-up Construction of Multifunctional Eukaryotic Synthetic Cells Göpfrich, Kerstin; Platzman, Ilia; Spatz, Joachim P. Trends in Biotechnology, Vol. 36, Issue 9 https://doi.org/10.1016/j.tibtech.2018.03.008	journal	September 2018
Simultaneous monitoring of transcription and translation in mammalian cell-free expression in bulk and in cell-sized droplets Wang, Shue; Majumder, Sagardip; Emery, Nicholas J. Synthetic Biology, Vol. 3, Issue 1 https://doi.org/10.1093/synbio/ysy005	journal	January 2018
Phenotype-Independent Isolation of Interspecies Saccharomyces Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting Gorter de Vries, Arthur R.; Koster, Charlotte C.; Weening, Susan M. Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00871	journal	April 2019
Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics Damiati, Samar; Mhanna, Rami; Kodzius, Rimantas Genes, Vol. 9, Issue 3 https://doi.org/10.3390/genes9030144	journal	March 2018
Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies Sato, Yusuke; Takinoue, Masahiro Micromachines, Vol. 10, Issue 4 https://doi.org/10.3390/mi10040216	journal	March 2019
Cytoskeletal and Actin‐Based Polymerization Motors and Their Role in Minimal Cell Design Hürtgen, Daniel; Vogel, Sven Kenjiro; Schwille, Petra Advanced Biosystems, Vol. 3, Issue 6 https://doi.org/10.1002/adbi.201800311	journal	April 2019
Programmable Functionalization of Surfactant‐Stabilized Microfluidic Droplets via DNA‐Tags Jahnke, Kevin; Weiss, Marian; Frey, Christoph Advanced Functional Materials, Vol. 29, Issue 23 https://doi.org/10.1002/adfm.201808647	journal	April 2019
Impact of electrode design and voltage waveform on low-potential magnetohydrodynamic fluid actuation Warner, Cael; McDermid, Cameron M.; Ahmadi, Ali Microfluidics and Nanofluidics, Vol. 23, Issue 7 https://doi.org/10.1007/s10404-019-2264-4	journal	June 2019
A flexible precise volume sensor based on metal-on-polyimide electrodes sandwiched by PDMS channel for microfluidic systems Pu, Zhihua; Ma, Jiaming; Li, Wenwen Microfluidics and Nanofluidics, Vol. 23, Issue 12 https://doi.org/10.1007/s10404-019-2300-4	journal	November 2019
Three-dimensional numerical simulation of droplet formation in a microfluidic flow-focusing device Han, Wenbo; Chen, Xueye; Wu, Zhongli Journal of the Brazilian Society of Mechanical Sciences and Engineering, Vol. 41, Issue 6 https://doi.org/10.1007/s40430-019-1767-y	journal	May 2019
Nanofiber membrane supported lung-on-a-chip microdevice for anti-cancer drug testing Yang, Xingyuan; Li, Kaiyan; Zhang, Xu Lab on a Chip, Vol. 18, Issue 3 https://doi.org/10.1039/c7lc01224a	journal	January 2018
High throughput screening of complex biological samples with mass spectrometry – from bulk measurements to single cell analysis Kempa, Emily E.; Hollywood, Katherine A.; Smith, Clive A. The Analyst, Vol. 144, Issue 3 https://doi.org/10.1039/c8an01448e	journal	January 2019
Fluorescence lifetime-activated droplet sorting in microfluidic chip systems Hasan, Sadat; Geissler, David; Wink, Konstantin Lab on a Chip, Vol. 19, Issue 3 https://doi.org/10.1039/c8lc01278d	journal	January 2019
Antifouling digital microfluidics using lubricant infused porous film Geng, Hongyao; Cho, Sung Kwon Lab on a Chip, Vol. 19, Issue 13 https://doi.org/10.1039/c9lc00289h	journal	January 2019
Liquid metal electrode-enabled flexible microdroplet sensor Zhang, Renchang; Ye, Zi; Gao, Meng Lab on a Chip, Vol. 20, Issue 3 https://doi.org/10.1039/c9lc00995g	journal	January 2020
Synthetic biology UK: progress, paradigms and prospects Clarke, Lionel J. Engineering Biology, Vol. 1, Issue 2 https://doi.org/10.1049/enb.2017.0022	journal	December 2017
New insights into generation of highly controllable monodisperse high-throughput microdroplets in a T-junction microchannel with step structure Han, Wenbo; Chen, Xueye Journal of Dispersion Science and Technology https://doi.org/10.1080/01932691.2019.1679643	journal	October 2019
Encapsulation of complex solutions using droplet microfluidics towards the synthesis of artificial cells Majumder, Sagardip; Wubshet, Nadab; Liu, Allen P. Journal of Micromechanics and Microengineering, Vol. 29, Issue 8 https://doi.org/10.1088/1361-6439/ab2377	journal	June 2019
Mass Transfer via Femtoliter Droplets in Ping-Pong Mode Shojaeian, Mostafa; Hardt, Steffen Physical Review Applied, Vol. 13, Issue 1 https://doi.org/10.1103/physrevapplied.13.014015	journal	January 2020
Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable Kothamachu, Varun B.; Zaini, Sabrina; Muffatto, Federico SLAS TECHNOLOGY: Translating Life Sciences Innovation https://doi.org/10.1177/2472630320931794	journal	June 2020
High-throughput magnetic particle washing in nanoliter droplets using serial injection and splitting Stephenson, William Micro and Nano Systems Letters, Vol. 6, Issue 1 https://doi.org/10.1186/s40486-018-0065-2	journal	June 2018
Label-Free Sensing in Microdroplet-Based Microfluidic Systems Kalantarifard, Ali; Saateh, Abtin; Elbuken, Caglar Chemosensors, Vol. 6, Issue 2 https://doi.org/10.3390/chemosensors6020023	journal	May 2018
The Usual Suspects 2019: of Chips, Droplets, Synthesis, and Artificial Cells Eilenberger, Christoph; Spitz, Sarah; Bachmann, Barbara Eva Maria Micromachines, Vol. 10, Issue 5 https://doi.org/10.3390/mi10050285	journal	April 2019

Figures / Tables (1)

Table 1(p. 12)

Similar Records

A Versatile Microfluidic Device for Automating Synthetic Biology

Journal Article · Mon Jun 15 00:00:00 EDT 2015 · ACS Synthetic Biology · OSTI ID:1378593

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Droplet microfluidics for synthetic biology

Citation Formats

References (126)

Cited By (23)

Figures / Tables (1)

Similar Records

Related Subjects