Adaptive evolution of genomically recoded Escherichia coli

Wannier, Timothy M.; Kunjapur, Aditya M.; Rice, Daniel P.; McDonald, Michael J.; Desai, Michael M.; Church, George M.

doi:10.1073/pnas.1715530115

Adaptive evolution of genomically recoded Escherichia coli

Journal Article · Tue Feb 13 00:00:00 EST 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1715530115· OSTI ID:1420357

Wannier, Timothy M. ^[1]; Kunjapur, Aditya M. ^[1]; Rice, Daniel P. ^[2]; McDonald, Michael J. ^[2]; Desai, Michael M. ^[2]; ^[1]

Department of Genetics, Harvard Medical School, Boston, MA 02115,
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138

Significance

The construction of an organism with an altered genetic code negatively impacted its fitness. We evolved this organism for ∼1,100 generations in the laboratory to recover fitness and learn what changes would accumulate during evolutionary trajectories toward faster growth rates. We observed several selective mutations that helped alleviate insufficient translation termination or that corrected for unintended mutations that accumulated when we originally altered the genetic code. Further observed mutations were generally adaptive in a nonrecoded background. This work bolsters our understanding of the pliability of the genetic code and will help guide future efforts seeking to recode genomes. Finally, it results in a useful strain for nonstandard amino acid incorporation in numerous contexts relevant for research and industry.

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Research Organization:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Organization:: Amazon Web Services Cloud Credits for Research Program; USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: FG02-02ER63445

OSTI ID:: 1420357

Alternate ID(s):: OSTI ID: 1527125

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 12 Vol. 115; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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