Functionalisation of Detonation Nanodiamond for Monodispersed, Soluble DNA-Nanodiamond Conjugates Using Mixed Silane Bead-Assisted Sonication Disintegration

Edgington, Robert; Spillane, Katelyn M.; Papageorgiou, George; Wray, William; Ishiwata, Hitoshi; Labarca, Mariana; Leal-Ortiz, Sergio; Reid, Gordon; Webb, Martin; Foord, John; Melosh, Nicholas; Schaefer, Andreas T.

doi:10.1038/s41598-017-18601-6

Functionalisation of Detonation Nanodiamond for Monodispersed, Soluble DNA-Nanodiamond Conjugates Using Mixed Silane Bead-Assisted Sonication Disintegration

Journal Article · Mon Jan 15 00:00:00 EST 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-18601-6· OSTI ID:1419981

Edgington, Robert ^[1]; Spillane, Katelyn M. ^[2]; Papageorgiou, George ^[3]; Wray, William ^[4]; Ishiwata, Hitoshi ^[5]; Labarca, Mariana ^[5]; Leal-Ortiz, Sergio ^[5]; Reid, Gordon ^[3]; Webb, Martin ^[3]; Foord, John ^[6]; Melosh, Nicholas ^[5]; Schaefer, Andreas T. ^[4]

The Francis Crick Institute, London (United Kingdom); Stanford Univ., Stanford, CA (United States); Univ. of Oxford, Oxford (United Kingdom)
The Francis Crick Institute, London (United Kingdom); King's College London, London (United Kingdom)
The Francis Crick Institute, London (United Kingdom)
The Francis Crick Institute, London (United Kingdom); Univ. College London, London (United Kingdom)
Stanford Univ., Stanford, CA (United States)
Univ. of Oxford, Oxford (United Kingdom)

Here, nanodiamonds have many attractive properties that make them suitable for a range of biological applications, but their practical use has been limited because nanodiamond conjugates tend to aggregate in solution during or after functionalisation. Here we demonstrate the production of DNA-detonation nanodiamond (DNA-DND) conjugates with high dispersion and solubility using an ultrasonic, mixed-silanization chemistry protocol based on the in situ Bead-Assisted Sonication Disintegration (BASD) silanization method. We use two silanes to achieve these properties: (1) 3-(trihydroxysilyl)propyl methylphosphonate (THPMP); a negatively charged silane that imparts high zeta potential and solubility in solution; and (2) (3-aminopropyl)triethoxysilane (APTES); a commonly used functional silane that contributes an amino group for subsequent bioconjugation. We target these amino groups for covalent conjugation to thiolated, single-stranded DNA oligomers using the heterobifunctional crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC). The resulting DNA-DND conjugates are the smallest reported to date, as determined by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). The functionalisation method we describe is versatile and can be used to produce a wide variety of soluble DND-biomolecule conjugates.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1419981

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 8; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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