Visualization of cellulases bound to cellulose microfibrils: Evidence for endo/exo synergism
Conference
·
OSTI ID:141961
- NREL, Golden, CO (United States)
- Colorado State Univ., Fort Collins, CO (United States)
Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10-nm and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I to cellulose was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immobilization of cellulose microfibrils on copper EM grids, incubation of the cellulose-coated grids with cellulase(s), binding of MAb-colloidal gold conjugates to cellulase(s), and visualization via transmission electron microscopy (TEM). Apparent differences in the substrate binding preferences for gold-labelled CBH I and EG I were observed; however, the actual structure of the cellulose at these sites was not confirmed. Sites showing binding of both enzymes sometimes showed patterns of coordination suggestive of the {open_quotes}progressive{close_quotes} cellulase action model. The sensitivity of this technique was evident when micrographs were observed in 3-D.
- OSTI ID:
- 141961
- Report Number(s):
- CONF-930304--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Visualization of Trichoderma reesei cellobiohydrolase I and endoglucanase I on aspen cellulose by using monoclonal antibody-colloidal gold conjugates
Cellulase: A key enzyme for fermentation feedstocks
Cellulase a key enzyme in fermentation: Annual report, 1985--1986
Journal Article
·
Thu Oct 31 23:00:00 EST 1991
· Applied and Environmental Microbiology; (United States)
·
OSTI ID:5883818
Cellulase: A key enzyme for fermentation feedstocks
Technical Report
·
Mon Aug 01 00:00:00 EDT 1988
·
OSTI ID:6810692
Cellulase a key enzyme in fermentation: Annual report, 1985--1986
Technical Report
·
Sun May 04 00:00:00 EDT 1986
·
OSTI ID:6838861