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Title: Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123

Abstract

Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at least one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation.

Authors:
 [1];  [2];  [1];  [2]; ORCiD logo [1]
  1. Department of Biological Chemistry, University of Michigan Medical School, Michigan, United States
  2. Structural Biology Laboratory of Epigenetics, Department of Biological Sciences, Graduate school of Nanoscience and Technology (World Class University), KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
NIHFOREIGNOTHER
OSTI Identifier:
1419062
Resource Type:
Journal Article
Resource Relation:
Journal Name: eLife; Journal Volume: 6; Journal Issue: 2017
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

An, Sojin, Yoon, Jungmin, Kim, Hanseong, Song, Ji-Joon, and Cho, Uhn-soo. Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123. United States: N. p., 2017. Web. doi:10.7554/eLife.30244.
An, Sojin, Yoon, Jungmin, Kim, Hanseong, Song, Ji-Joon, & Cho, Uhn-soo. Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123. United States. doi:10.7554/eLife.30244.
An, Sojin, Yoon, Jungmin, Kim, Hanseong, Song, Ji-Joon, and Cho, Uhn-soo. Mon . "Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123". United States. doi:10.7554/eLife.30244.
@article{osti_1419062,
title = {Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123},
author = {An, Sojin and Yoon, Jungmin and Kim, Hanseong and Song, Ji-Joon and Cho, Uhn-soo},
abstractNote = {Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at least one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation.},
doi = {10.7554/eLife.30244},
journal = {eLife},
number = 2017,
volume = 6,
place = {United States},
year = {Mon Oct 16 00:00:00 EDT 2017},
month = {Mon Oct 16 00:00:00 EDT 2017}
}