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Title: Constructs and methods for genome editing and genetic engineering of fungi and protists

Abstract

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Inventors:
;
Publication Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1418981
Patent Number(s):
9,879,270
Application Number:
14/826,566
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Aug 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hittinger, Christopher Todd, and Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States: N. p., 2018. Web.
Hittinger, Christopher Todd, & Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States.
Hittinger, Christopher Todd, and Alexander, William Gerald. Tue . "Constructs and methods for genome editing and genetic engineering of fungi and protists". United States. doi:. https://www.osti.gov/servlets/purl/1418981.
@article{osti_1418981,
title = {Constructs and methods for genome editing and genetic engineering of fungi and protists},
author = {Hittinger, Christopher Todd and Alexander, William Gerald},
abstractNote = {Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 30 00:00:00 EST 2018},
month = {Tue Jan 30 00:00:00 EST 2018}
}

Patent:

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