Constructs and methods for genome editing and genetic engineering of fungi and protists

Hittinger, Christopher Todd; Alexander, William Gerald

Title: Constructs and methods for genome editing and genetic engineering of fungi and protists

Patent · Tue Jan 30 00:00:00 EST 2018

OSTI ID:1418981

Hittinger, Christopher Todd; Alexander, William Gerald

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

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Research Organization:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 9,879,270

Application Number:: 14/826,566

OSTI ID:: 1418981

Resource Relation:: Patent File Date: 2015 Aug 14

Country of Publication:: United States

Language:: English

References (8)

Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription Doudna, Jennifer A.; Jinek, Martin; Charpentier, Emmanuelle https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140068797 US Patent Application 13/842859; 20140068797	patent-application	March 2014
Cryptosporidium Transfection Methods And Transfected Cryptosporidium Cells Striepen, Boris; Vinayak, Sumiti; Brooks, Carrie F. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160160224 US Patent Application 14/905907; 20160160224	patent-application	June 2016
High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces Alexander, W. G.; Doering, D. T.; Hittinger, C. T. Genetics, Vol. 198, Issue 3, p. 859-866 https://doi.org/10.1534/genetics.114.170118	journal	September 2014
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems DiCarlo, James E.; Norville, Julie E.; Mali, Prashant Nucleic Acids Research, Vol. 41, Issue 7, p. 4336-4343 https://doi.org/10.1093/nar/gkt135	journal	March 2013
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering Gaj, Thomas; Gersbach, Charles A.; Barbas, Carlos F. Trends in Biotechnology, Vol. 31, Issue 7, p. 397-405 https://doi.org/10.1016/j.tibtech.2013.04.004	journal	July 2013
The CRISPR Craze Pennisi, E. Science, Vol. 341, Issue 6148, p. 833-836 https://doi.org/10.1126/science.341.6148.833	journal	August 2013
In vivo site-directed mutagenesis using oligonucleotides Storici, Francesca; Lewis, L. Kevin; Resnick, Michael A. Nature Biotechnology, Vol. 19, Issue 8, p. 773-776 https://doi.org/10.1038/90837	journal	August 2001
Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae Stuckey, Samantha; Storici, Francesca Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate https://doi.org/10.1016/B978-0-12-420067-8.00008-8	book	January 2013

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