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Title: Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii

Abstract

Sedum alfredii is one of a few plant species known to hyperaccumulate cadmium (Cd). Uptake, localization, and tolerance of Cd at cellular levels in shoots were compared in hyperaccumulating (HE) and non-hyperaccumulating (NHE) ecotypes of Sedum alfredii. X-ray fluorescence images of Cd in stems and leaves showed only a slight Cd signal restricted within vascular bundles in the NHEs, while enhanced localization of Cd, with significant tissue- and age-dependent variations, was detected in HEs. In contrast to the vascular-enriched Cd in young stems, parenchyma cells in leaf mesophyll, stem pith and cortex tissues served as terminal storage sites for Cd sequestration in HEs. Kinetics of Cd transport into individual leaf protoplasts of the two ecotypes showed little difference in Cd accumulation. However, far more efficient storage of Cd in vacuoles was apparent in HEs. Subsequent analysis of cell viability and hydrogen peroxide levels suggested that HE protoplasts exhibited higher resistance to Cd than those of NHE protoplasts. These results suggest that efficient sequestration into vacuoles, as opposed to rapid transport into parenchyma cells, is a pivotal process in Cd accumulation and homeostasis in shoots of HE S. alfredii. This is in addition to its efficient root-to-shoot translocation of Cd.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGN
OSTI Identifier:
1417381
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Experimental Botany; Journal Volume: 68; Journal Issue: 9
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tian, Shengke, Xie, Ruohan, Wang, Haixin, Hu, Yan, Hou, Dandi, Liao, Xingcheng, Brown, Patrick H., Yang, Hongxia, Lin, Xianyong, Labavitch, John M., and Lu, Lingli. Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii. United States: N. p., 2017. Web. doi:10.1093/jxb/erx112.
Tian, Shengke, Xie, Ruohan, Wang, Haixin, Hu, Yan, Hou, Dandi, Liao, Xingcheng, Brown, Patrick H., Yang, Hongxia, Lin, Xianyong, Labavitch, John M., & Lu, Lingli. Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii. United States. doi:10.1093/jxb/erx112.
Tian, Shengke, Xie, Ruohan, Wang, Haixin, Hu, Yan, Hou, Dandi, Liao, Xingcheng, Brown, Patrick H., Yang, Hongxia, Lin, Xianyong, Labavitch, John M., and Lu, Lingli. Sat . "Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii". United States. doi:10.1093/jxb/erx112.
@article{osti_1417381,
title = {Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii},
author = {Tian, Shengke and Xie, Ruohan and Wang, Haixin and Hu, Yan and Hou, Dandi and Liao, Xingcheng and Brown, Patrick H. and Yang, Hongxia and Lin, Xianyong and Labavitch, John M. and Lu, Lingli},
abstractNote = {Sedum alfredii is one of a few plant species known to hyperaccumulate cadmium (Cd). Uptake, localization, and tolerance of Cd at cellular levels in shoots were compared in hyperaccumulating (HE) and non-hyperaccumulating (NHE) ecotypes of Sedum alfredii. X-ray fluorescence images of Cd in stems and leaves showed only a slight Cd signal restricted within vascular bundles in the NHEs, while enhanced localization of Cd, with significant tissue- and age-dependent variations, was detected in HEs. In contrast to the vascular-enriched Cd in young stems, parenchyma cells in leaf mesophyll, stem pith and cortex tissues served as terminal storage sites for Cd sequestration in HEs. Kinetics of Cd transport into individual leaf protoplasts of the two ecotypes showed little difference in Cd accumulation. However, far more efficient storage of Cd in vacuoles was apparent in HEs. Subsequent analysis of cell viability and hydrogen peroxide levels suggested that HE protoplasts exhibited higher resistance to Cd than those of NHE protoplasts. These results suggest that efficient sequestration into vacuoles, as opposed to rapid transport into parenchyma cells, is a pivotal process in Cd accumulation and homeostasis in shoots of HE S. alfredii. This is in addition to its efficient root-to-shoot translocation of Cd.},
doi = {10.1093/jxb/erx112},
journal = {Journal of Experimental Botany},
number = 9,
volume = 68,
place = {United States},
year = {Sat Apr 01 00:00:00 EDT 2017},
month = {Sat Apr 01 00:00:00 EDT 2017}
}