Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases

Zhang, Tao; Datta, Supratim; Simmons, Blake A.; Rubin, Edward M.

Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases

Patent · 09 January 2018

OSTI ID:1416354

Zhang, Tao; Datta, Supratim; Simmons, Blake A.; Rubin, Edward M.

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA); Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 9,862,982

Application Number:: 15/164,813

OSTI ID:: 1416354

Country of Publication:: United States

Language:: English

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