SPRUCE Deep Peat Microbial Diversity, CO2 and CH4 Production in Response to Nutrient, Temperature, and pH Treatments during Incubation Studies.

Dataset · Mon Sep 01 04:00:00 EDT 2014

DOI:https://doi.org/10.3334/CDIAC/spruce.040· OSTI ID:1415734

A., Kluber Laurel ^[1]; Allen, Samantha A. ^[2]; Hendershot, Nicholas ^[2]; Hanson, Paul J. ^[2]; Schadt, Christopher W. ^[2]

---

1. Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A.; ORNL
2. Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A.

This data set contains the results of a microcosm incubation study on deep peat collected from the SPRUCE experimental site in the S1 Bog in September 2014. Microcosms were monitored for CO2 and CH4 production, and microbial community dynamics were assessed using qPCR and amplicon sequencing.The experiment was designed with a full factorial design with elevated temperature, nitrogen (N), (P), and pH treatments was used with samples from each transect serving replicates. In all, 96 microcosms were constructed to account for the 16 treatment combinations (N x P x pH x temperature), 2 time points, and 3 replicates. Temperature treatments were 6 °C, to mimic the SPRUCE ambient plot temperatures, and 15 °C to mimic the SPRUCE +9 °C treatment.

• Research Organization: ORNLTESSFA (Oak Ridge National Lab's Terrestrial Ecosystem Science Scientific Focus Area (ORNL TES SFA))
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Climate and Environmental Sciences Division
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1415734
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES