Microorganisms and methods for producing pyruvate, ethanol, and other compounds

Reed, Jennifer L.; Zhang, Xiaolin

Microorganisms and methods for producing pyruvate, ethanol, and other compounds

Patent · 25 December 2017

OSTI ID:1415026

Reed, Jennifer L.; Zhang, Xiaolin

Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.

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Research Organization:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494; SC0008103

Assignee:: WISCONSIN ALUMNI RESEARCH FOUNDATION (Madison, WI)

Patent Number(s):: 9,850,505

Application Number:: 14/848,646

OSTI ID:: 1415026

Country of Publication:: United States

Language:: English

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