Quantification of triglyceride content in oleaginous materials using thermo-gravimetry
- Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering; Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering
- Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering; Manhanttan College, Riverdale, NY (United States). Dept. of Chemical Engineering
Laboratory analytical methods for quantification of triglyceride content in oleaginous biomass samples, especially microalgae, require toxic chemicals and/or organic solvents and involve multiple steps. We describe a simple triglyceride quantification method that uses thermo-gravimetry. This method is based on the observation that triglycerides undergo near-complete volatilization/degradation over a narrow temperature interval with a derivative weight loss peak at 420 °C when heated in an inert atmosphere. Degradation of the other constituents of oleaginous biomass (protein and carbohydrates) is largely complete after prolonged exposure of samples at 320 °C. Based on these observations, the triglyceride content of oleaginous biomass was estimated by using the following two-step process. In Step 1, samples were heated to 320 °C and kept isothermal at this temperature for 15 min. In Step 2, samples were heated from 320 °C to 420 °C and then kept isothermal at 420 °C for 15 min. The results show that mass loss in step 2 correlated well with triglyceride content estimates obtained from conventional techniques for diverse microalgae and oilseed samples.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- Grant/Contract Number:
- AC0576RL01830; CHE-1230609; EE0005993
- OSTI ID:
- 1413483
- Journal Information:
- Journal of Analytical and Applied Pyrolysis, Vol. 128, Issue C; ISSN 0165-2370
- Publisher:
- ElsevierCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Similar Records
Flash Pyrolysis of Oleaginous Biomass in a Fluidized-Bed Reactor
Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana