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Title: Quantification of triglyceride content in oleaginous materials using thermo-gravimetry

Abstract

Laboratory analytical methods for quantification of triglyceride content in oleaginous biomass samples, especially microalgae, require toxic chemicals and/or organic solvents and involve multiple steps. We describe a simple triglyceride quantification method that uses thermo-gravimetry. This method is based on the observation that triglycerides undergo near-complete volatilization/degradation over a narrow temperature interval with a derivative weight loss peak at 420 °C when heated in an inert atmosphere. Degradation of the other constituents of oleaginous biomass (protein and carbohydrates) is largely complete after prolonged exposure of samples at 320 °C. Based on these observations, the triglyceride content of oleaginous biomass was estimated by using the following two-step process. In Step 1, samples were heated to 320 °C and kept isothermal at this temperature for 15 min. In Step 2, samples were heated from 320 °C to 420 °C and then kept isothermal at 420 °C for 15 min. The results show that mass loss in step 2 correlated well with triglyceride content estimates obtained from conventional techniques for diverse microalgae and oilseed samples.

Authors:
 [1];  [2];  [2];  [3]
  1. Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering; Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering
  3. Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering; Manhanttan College, Riverdale, NY (United States). Dept. of Chemical Engineering
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1413483
Grant/Contract Number:
AC0576RL01830; CHE-1230609; EE0005993
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Analytical and Applied Pyrolysis
Additional Journal Information:
Journal Volume: 128; Journal Issue: C; Journal ID: ISSN 0165-2370
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 09 BIOMASS FUELS; triglyceride quantification; lipid analysis; thermo-gravimetry; microalgae; biomass; oilseeds

Citation Formats

Maddi, Balakrishna, Vadlamani, Agasteswar, Viamajala, Sridhar, and Varanasi, Sasidhar. Quantification of triglyceride content in oleaginous materials using thermo-gravimetry. United States: N. p., 2017. Web. doi:10.1016/J.JAAP.2017.10.006.
Maddi, Balakrishna, Vadlamani, Agasteswar, Viamajala, Sridhar, & Varanasi, Sasidhar. Quantification of triglyceride content in oleaginous materials using thermo-gravimetry. United States. doi:10.1016/J.JAAP.2017.10.006.
Maddi, Balakrishna, Vadlamani, Agasteswar, Viamajala, Sridhar, and Varanasi, Sasidhar. Mon . "Quantification of triglyceride content in oleaginous materials using thermo-gravimetry". United States. doi:10.1016/J.JAAP.2017.10.006.
@article{osti_1413483,
title = {Quantification of triglyceride content in oleaginous materials using thermo-gravimetry},
author = {Maddi, Balakrishna and Vadlamani, Agasteswar and Viamajala, Sridhar and Varanasi, Sasidhar},
abstractNote = {Laboratory analytical methods for quantification of triglyceride content in oleaginous biomass samples, especially microalgae, require toxic chemicals and/or organic solvents and involve multiple steps. We describe a simple triglyceride quantification method that uses thermo-gravimetry. This method is based on the observation that triglycerides undergo near-complete volatilization/degradation over a narrow temperature interval with a derivative weight loss peak at 420 °C when heated in an inert atmosphere. Degradation of the other constituents of oleaginous biomass (protein and carbohydrates) is largely complete after prolonged exposure of samples at 320 °C. Based on these observations, the triglyceride content of oleaginous biomass was estimated by using the following two-step process. In Step 1, samples were heated to 320 °C and kept isothermal at this temperature for 15 min. In Step 2, samples were heated from 320 °C to 420 °C and then kept isothermal at 420 °C for 15 min. The results show that mass loss in step 2 correlated well with triglyceride content estimates obtained from conventional techniques for diverse microalgae and oilseed samples.},
doi = {10.1016/J.JAAP.2017.10.006},
journal = {Journal of Analytical and Applied Pyrolysis},
number = C,
volume = 128,
place = {United States},
year = {Mon Oct 16 00:00:00 EDT 2017},
month = {Mon Oct 16 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on October 16, 2018
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