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Title: S5H/DMR6 Encodes a Salicylic Acid 5-Hydroxylase That Fine-Tunes Salicylic Acid Homeostasis

Abstract

The phytohormone salicylic acid (SA) plays essential roles in biotic and abiotic responses, plant development, and leaf senescence. 2,5-Dihydroxybenzoic acid (2,5-DHBA or gentisic acid) is one of the most commonly occurring aromatic acids in green plants and is assumed to be generated from SA, but the enzymes involved in its production remain obscure. DMR6 (Downy Mildew Resistant 6, At5g24530) has been proven essential in plant immunity of Arabidopsis, but its biochemical properties are not well understood. Here in this paper, we report the discovery and functional characterization of DMR6 as a SA 5-hydroxylase (S5H) that catalyzes the formation of 2,5-DHBA by hydroxylating SA at the C5 position of its phenyl ring in Arabidopsis. S5H/DMR6 specifically converts SA to 2,5-DHBA in vitro and displays higher catalytic efficiency (K cat/K m=4.96×10 4 M -1s -1) than the previously reported SA 3-hydroxylase (S3H, K cat/K m=6.09 × 10 3 M -1s -1) for SA. Interestingly, S5H/DMR6 displays a substrate inhibition property that may enable automatic control of its enzyme activities. The s5h mutant and s5hs3h double mutant over accumulate SA and display phenotypes such as a smaller growth size, early senescence and a loss of susceptibility to Pseudomonas syringae pv. tomato DC3000 (Pstmore » DC3000). S5H/DMR6 is sensitively induced by SA/pathogen treatment and is widely expressed from young seedlings to senescing plants, whereas S3H is more specifically expressed at the mature and senescing stages. Collectively, our results disclose the identity of the enzyme required for 2,5-DHBA formation and reveal a mechanism by which plants fine-tune SA homeostasis by mediating SA 5-hydroxylation.« less

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [1];  [1];  [2]; ORCiD logo [3]; ORCiD logo [1]
  1. Zhejiang Normal Univ., Jinhua, Zhejiang (China). Inst. of Plant Genetics and Developmental Biology, College of Chemistry and Life Sciences
  2. Cornell Univ., Ithaca, NY (United States). Plant Biology Section, School of Integrative Plant Science
  3. Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Biosciences
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Natural Science Foundation of China (NNSFC); Natural Science Foundation of Zhejiang Province
OSTI Identifier:
1412669
Alternate Identifier(s):
OSTI ID: 1389878
Report Number(s):
BNL-114465-2017-JA
Journal ID: ISSN 0032-0889
Grant/Contract Number:
SC0012704; AC02-98CH10886
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda); Journal Volume: 175; Journal Issue: 3; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhang, Yanjun, Zhao, Li, Zhao, Jiangzhe, Li, Yujia, Wang, Jinbin, Guo, Rong, Gan, Susheng, Liu, Chang-Jun, and Zhang, Kewei. S5H/DMR6 Encodes a Salicylic Acid 5-Hydroxylase That Fine-Tunes Salicylic Acid Homeostasis. United States: N. p., 2017. Web. doi:10.1104/pp.17.00695.
Zhang, Yanjun, Zhao, Li, Zhao, Jiangzhe, Li, Yujia, Wang, Jinbin, Guo, Rong, Gan, Susheng, Liu, Chang-Jun, & Zhang, Kewei. S5H/DMR6 Encodes a Salicylic Acid 5-Hydroxylase That Fine-Tunes Salicylic Acid Homeostasis. United States. doi:10.1104/pp.17.00695.
Zhang, Yanjun, Zhao, Li, Zhao, Jiangzhe, Li, Yujia, Wang, Jinbin, Guo, Rong, Gan, Susheng, Liu, Chang-Jun, and Zhang, Kewei. Tue . "S5H/DMR6 Encodes a Salicylic Acid 5-Hydroxylase That Fine-Tunes Salicylic Acid Homeostasis". United States. doi:10.1104/pp.17.00695. https://www.osti.gov/servlets/purl/1412669.
@article{osti_1412669,
title = {S5H/DMR6 Encodes a Salicylic Acid 5-Hydroxylase That Fine-Tunes Salicylic Acid Homeostasis},
author = {Zhang, Yanjun and Zhao, Li and Zhao, Jiangzhe and Li, Yujia and Wang, Jinbin and Guo, Rong and Gan, Susheng and Liu, Chang-Jun and Zhang, Kewei},
abstractNote = {The phytohormone salicylic acid (SA) plays essential roles in biotic and abiotic responses, plant development, and leaf senescence. 2,5-Dihydroxybenzoic acid (2,5-DHBA or gentisic acid) is one of the most commonly occurring aromatic acids in green plants and is assumed to be generated from SA, but the enzymes involved in its production remain obscure. DMR6 (Downy Mildew Resistant 6, At5g24530) has been proven essential in plant immunity of Arabidopsis, but its biochemical properties are not well understood. Here in this paper, we report the discovery and functional characterization of DMR6 as a SA 5-hydroxylase (S5H) that catalyzes the formation of 2,5-DHBA by hydroxylating SA at the C5 position of its phenyl ring in Arabidopsis. S5H/DMR6 specifically converts SA to 2,5-DHBA in vitro and displays higher catalytic efficiency (Kcat/Km=4.96×104 M-1s-1) than the previously reported SA 3-hydroxylase (S3H, Kcat/Km=6.09 × 103 M-1s-1) for SA. Interestingly, S5H/DMR6 displays a substrate inhibition property that may enable automatic control of its enzyme activities. The s5h mutant and s5hs3h double mutant over accumulate SA and display phenotypes such as a smaller growth size, early senescence and a loss of susceptibility to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). S5H/DMR6 is sensitively induced by SA/pathogen treatment and is widely expressed from young seedlings to senescing plants, whereas S3H is more specifically expressed at the mature and senescing stages. Collectively, our results disclose the identity of the enzyme required for 2,5-DHBA formation and reveal a mechanism by which plants fine-tune SA homeostasis by mediating SA 5-hydroxylation.},
doi = {10.1104/pp.17.00695},
journal = {Plant Physiology (Bethesda)},
number = 3,
volume = 175,
place = {United States},
year = {Tue Sep 12 00:00:00 EDT 2017},
month = {Tue Sep 12 00:00:00 EDT 2017}
}

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