skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Correction: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells

Journal Article · · eLife
DOI:https://doi.org/10.7554/eLife.10328· OSTI ID:1411719
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of California, San Diego, CA (United States). Division of Biological Sciences, Cel and Developmental Biology Section
+ Show Author Affiliations

A central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca2+-dependent and Ca2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca2+-signaling on a cellular, genetic, and biochemical level.

Research Organization:
Univ. of California, San Diego, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
FG02-03ER15449
OSTI ID:
1411719
Journal Information:
eLife, Vol. 4; ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.Copyright Statement
Country of Publication:
United States
Language:
English

References (2)

Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells journal July 2015
Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells journal July 2015

Cited By (3)

Blue light and CO2 signals converge to regulate light-induced stomatal opening journal November 2017
Spatio-temporal aspects of Ca2+ signalling: lessons from guard cells and pollen tubes journal April 2018
PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance journal February 2017

Similar Records

Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells
Journal Article · Mon Jul 20 00:00:00 EDT 2015 · eLife · OSTI ID:1411719
+8 more
Plant, cell, and molecular mechanisms of abscisic-acid regulation of stomatal apertures. In vivo phosphorylation of phosphoenolpyruvate carboxylase in guard cells of Vicia faba L. is enhanced by fusicoccin and suppressed by abscisic acid
Technical Report · Tue Dec 31 00:00:00 EST 1996 · OSTI ID:1411719
Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses
Journal Article · Fri Sep 04 00:00:00 EDT 2015 · Plant Physiology (Bethesda) · OSTI ID:1411719
+11 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES