Bioconversion of distillers’ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture

Liu, Fang; Wu, Weihua; Tran-Gyamfi, Mary B.; Jaryenneh, James D.; Zhuang, Xun; Davis, Ryan W.

doi:10.1186/s12934-017-0804-8

Bioconversion of distillers’ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture

Journal Article · Wed Nov 08 23:00:00 EST 2017 · Microbial Cell Factories

DOI:https://doi.org/10.1186/s12934-017-0804-8· OSTI ID:1411235

^[1]; Wu, Weihua ^[1]; Tran-Gyamfi, Mary B. ^[1]; Jaryenneh, James D. ^[1]; Zhuang, Xun ^[1]; Davis, Ryan W. ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Here, the first generation bioethanol production utilizes the starch fraction of maize, which accounts for approximately 60% of the ash-free dry weight of the grain. Scale-up of this technology for fuels applications has resulted in a massive supply of distillers’ grains with solubles (DGS) coproduct, which is rich in cellulosic polysaccharides and protein. It was surmised that DGS would be rapidly adopted for animal feed applications, however, this has not been observed based on inconsistency of the product stream and other logistics-related risks, especially toxigenic contaminants. Therefore, efficient valorization of DGS for production of petroleum displacing products will significantly improve the techno-economic feasibility and net energy return of the established starch bioethanol process. In this study, we demonstrate ‘one-pot’ bioconversion of the protein and carbohydrate fractions of a DGS hydrolysate into C4 and C5 fusel alcohols through development of a microbial consortium incorporating two engineered Escherichia coli biocatalyst strains.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1411235

Report Number(s):: SAND--2017-12329J; PII: 804

Journal Information:: Microbial Cell Factories, Journal Name: Microbial Cell Factories Journal Issue: 1 Vol. 16; ISSN 1475-2859

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Bioconversion of distillers’ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture [Supplemental Data] Liu, Fang; Tran-Gyamfi, Mary; Jaryenneh, James https://doi.org/10.6084/M9.FIGSHARE.C.3927010 The current record is supplemented by this figure	figure	November 2017
Bioconversion of distillers’ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture Liu, Fang; Wu, Weihua; Tran-Gyamfi, Mary https://doi.org/10.6084/m9.figshare.c.3927010.v1 The current record is supplemented by this dataset	dataset	November 2017
MOESM1 of Bioconversion of distillersâ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture Liu, Fang; Wu, Weihua; Tran-Gyamfi, Mary https://doi.org/10.6084/m9.figshare.c.3927010_d1 The current record is supplemented by this text	text	January 2017
MOESM1 of Bioconversion of distillersâ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture Liu, Fang; Wu, Weihua; Tran-Gyamfi, Mary https://doi.org/10.6084/m9.figshare.c.3927010_d1.v1 The current record is supplemented by this dataset	dataset	November 2017

Bioconversion of distillers’ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture

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