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Title: Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Abstract

Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: sugar release, physicochemical properties, and relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. As a result, the trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [2]; ORCiD logo [1];  [1];  [1]; ORCiD logo [1];  [2]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [3];  [4];  [5]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [6]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Georgia Inst. of Technology, Atlanta, GA (United States)
  3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  4. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
  5. West Virginia Univ., Morgantown, WV (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1410915
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Green Chemistry
Additional Journal Information:
Journal Volume: 19; Journal Issue: 22; Journal ID: ISSN 1463-9262
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Yoo, Chang Geun, Yang, Yongil, Pu, Yunqiao, Meng, Xianzhi, Muchero, Wellington, Yee, Kelsey L., Thompson, Olivia A., Rodriguez, Jr., Miguel, Bali, Garima, Engle, Nancy L., Lindquist, Erika, Singan, Vasanth, Schmutz, Jeremy, DiFazio, Stephen P., Tschaplinski, Timothy J., Tuskan, Gerald A., Chen, Jin -Gui, Davison, Brian, and Ragauskas, Arthur J. Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion. United States: N. p., 2017. Web. doi:10.1039/C7GC02219K.
Yoo, Chang Geun, Yang, Yongil, Pu, Yunqiao, Meng, Xianzhi, Muchero, Wellington, Yee, Kelsey L., Thompson, Olivia A., Rodriguez, Jr., Miguel, Bali, Garima, Engle, Nancy L., Lindquist, Erika, Singan, Vasanth, Schmutz, Jeremy, DiFazio, Stephen P., Tschaplinski, Timothy J., Tuskan, Gerald A., Chen, Jin -Gui, Davison, Brian, & Ragauskas, Arthur J. Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion. United States. doi:10.1039/C7GC02219K.
Yoo, Chang Geun, Yang, Yongil, Pu, Yunqiao, Meng, Xianzhi, Muchero, Wellington, Yee, Kelsey L., Thompson, Olivia A., Rodriguez, Jr., Miguel, Bali, Garima, Engle, Nancy L., Lindquist, Erika, Singan, Vasanth, Schmutz, Jeremy, DiFazio, Stephen P., Tschaplinski, Timothy J., Tuskan, Gerald A., Chen, Jin -Gui, Davison, Brian, and Ragauskas, Arthur J. Mon . "Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion". United States. doi:10.1039/C7GC02219K.
@article{osti_1410915,
title = {Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion},
author = {Yoo, Chang Geun and Yang, Yongil and Pu, Yunqiao and Meng, Xianzhi and Muchero, Wellington and Yee, Kelsey L. and Thompson, Olivia A. and Rodriguez, Jr., Miguel and Bali, Garima and Engle, Nancy L. and Lindquist, Erika and Singan, Vasanth and Schmutz, Jeremy and DiFazio, Stephen P. and Tschaplinski, Timothy J. and Tuskan, Gerald A. and Chen, Jin -Gui and Davison, Brian and Ragauskas, Arthur J.},
abstractNote = {Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: sugar release, physicochemical properties, and relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. As a result, the trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.},
doi = {10.1039/C7GC02219K},
journal = {Green Chemistry},
issn = {1463-9262},
number = 22,
volume = 19,
place = {United States},
year = {2017},
month = {10}
}

Works referenced in this record:

Nonenzymatic Sugar Production from Biomass Using Biomass-Derived  -Valerolactone
journal, January 2014


Transcriptome-Wide Identification of miRNA Targets under Nitrogen Deficiency in Populus tomentosa Using Degradome Sequencing
journal, June 2015

  • Chen, Min; Bao, Hai; Wu, Qiuming
  • International Journal of Molecular Sciences, Vol. 16, Issue 12
  • DOI: 10.3390/ijms160613937

Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments
journal, January 2013


Use of the Simons' Staining Technique to Assess Cellulose Accessibility in Pretreated Substrates
journal, August 2012

  • Chandra, Richard P.; Saddler, Jack N.
  • Industrial Biotechnology, Vol. 8, Issue 4
  • DOI: 10.1089/ind.2012.0016

Phylogeny and structure of the cinnamyl alcohol dehydrogenase gene family in Brachypodium distachyon
journal, October 2012

  • Bukh, Christian; Nord-Larsen, Pia Haugaard; Rasmussen, Søren K.
  • Journal of Experimental Botany, Vol. 63, Issue 17
  • DOI: 10.1093/jxb/ers275

Coming of age: ten years of next-generation sequencing technologies
journal, May 2016

  • Goodwin, Sara; McPherson, John D.; McCombie, W. Richard
  • Nature Reviews Genetics, Vol. 17, Issue 6
  • DOI: 10.1038/nrg.2016.49

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Transcriptome Analysis of Poplar during Leaf Spot Infection with Sphaerulina spp.
journal, September 2015


The strong association of condensed phenolic moieties in isolated lignins with their inhibition of enzymatic hydrolysis
journal, January 2016

  • Sun, Shaolong; Huang, Yang; Sun, Runcang
  • Green Chemistry, Vol. 18, Issue 15
  • DOI: 10.1039/C6GC00685J

Effects of organosolv and ammonia pretreatments on lignin properties and its inhibition for enzymatic hydrolysis
journal, January 2017

  • Yoo, Chang Geun; Li, Mi; Meng, Xianzhi
  • Green Chemistry, Vol. 19, Issue 8
  • DOI: 10.1039/C6GC03627A

featureCounts: an efficient general purpose program for assigning sequence reads to genomic features
journal, November 2013


Ultralight and highly flexible aerogels with long cellulose I nanofibers
journal, January 2011

  • Chen, Wenshuai; Yu, Haipeng; Li, Qing
  • Soft Matter, Vol. 7, Issue 21
  • DOI: 10.1039/c1sm06179h

Excellent rheological performance and impact toughness of cellulose nanofibers/PLA/ionomer composite
journal, January 2017

  • Li, Jingjing; Li, Jian; Feng, Dejun
  • RSC Advances, Vol. 7, Issue 46
  • DOI: 10.1039/C7RA04302C

Poplar as a feedstock for biofuels: A review of compositional characteristics
journal, March 2010

  • Sannigrahi, Poulomi; Ragauskas, Arthur J.; Tuskan, Gerald A.
  • Biofuels, Bioproducts and Biorefining, Vol. 4, Issue 2
  • DOI: 10.1002/bbb.206

Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011


Comparative interrogation of the developing xylem transcriptomes of two wood-forming species: Populus trichocarpa and Eucalyptus grandis
journal, February 2015

  • Hefer, Charles A.; Mizrachi, Eshchar; Myburg, Alexander A.
  • New Phytologist, Vol. 206, Issue 4
  • DOI: 10.1111/nph.13277

Role of Functional Groups in Lignin Inhibition of Enzymatic Hydrolysis of Cellulose to Glucose
journal, March 2008


Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass
journal, January 2017


Rho family GTPase-dependent immunity in plants and animals
journal, October 2014

  • Kawano, Yoji; Kaneko-Kawano, Takako; Shimamoto, Ko
  • Frontiers in Plant Science, Vol. 5
  • DOI: 10.3389/fpls.2014.00522

High-throughput analysis of transcriptome variation during water deficit in a poplar hybrid: a general overview
journal, September 2013

  • Cossu, Rosa Maria; Giordani, Tommaso; Cavallini, Andrea
  • Tree Genetics & Genomes, Vol. 10, Issue 1
  • DOI: 10.1007/s11295-013-0661-5

Development of a decision support tool for the assessment of biofuels
journal, March 2011


Enzymatic hydrolysis of organosolv Kanlow switchgrass and its impact on cellulose crystallinity and degree of polymerization
journal, January 2011

  • Cateto, Carolina; Hu, Gang; Ragauskas, Arthur
  • Energy & Environmental Science, Vol. 4, Issue 4
  • DOI: 10.1039/c0ee00827c

The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray)
journal, September 2006


Effect of dilute acid pretreatment of rice straw on structural properties and enzymatic hydrolysis
journal, July 2010


Lignin content in natural Populus variants affects sugar release
journal, March 2011

  • Studer, M. H.; DeMartini, J. D.; Davis, M. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
  • DOI: 10.1073/pnas.1009252108

Influence of Syringyl to Guaiacyl Ratio on the Structure of Natural and Synthetic Lignins
journal, January 2010

  • Kishimoto, Takao; Chiba, Wakako; Saito, Kaori
  • Journal of Agricultural and Food Chemistry, Vol. 58, Issue 2
  • DOI: 10.1021/jf9035172

Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
journal, August 2015


Biomass as renewable feedstock in standard refinery units. Feasibility, opportunities and challenges
journal, January 2012

  • Melero, Juan Antonio; Iglesias, Jose; Garcia, Alicia
  • Energy & Environmental Science, Vol. 5, Issue 6
  • DOI: 10.1039/c2ee21231e

Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013

  • Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-71

Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014


Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010

  • Park, Sunkyu; Baker, John O.; Himmel, Michael E.
  • Biotechnology for Biofuels, Vol. 3, Issue 1
  • DOI: 10.1186/1754-6834-3-10

High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus
journal, January 2015


Enzymatic breakdown of biomass: enzyme active sites, immobilization, and biofuel production
journal, January 2014


Formaldehyde stabilization facilitates lignin monomer production during biomass depolymerization
journal, October 2016

  • Shuai, Li; Amiri, Masoud Talebi; Questell-Santiago, Ydna M.
  • Science, Vol. 354, Issue 6310
  • DOI: 10.1126/science.aaf7810

Genetic architecture of growth traits in Populus revealed by integrated quantitative trait locus ( QTL ) analysis and association studies
journal, October 2015

  • Du, Qingzhang; Gong, Chenrui; Wang, Qingshi
  • New Phytologist, Vol. 209, Issue 3
  • DOI: 10.1111/nph.13695

Comparison of laboratory delignification methods, their selectivity, and impacts on physiochemical characteristics of cellulosic biomass
journal, February 2013


Naturally p-Hydroxybenzoylated Lignins in Palms
journal, February 2015


Emerging strategies for breaking the 3D amorphous network of lignin
journal, January 2014

  • Dutta, Saikat; Wu, Kevin C. -W.; Saha, Basudeb
  • Catal. Sci. Technol., Vol. 4, Issue 11
  • DOI: 10.1039/C4CY00701H

HISAT: a fast spliced aligner with low memory requirements
journal, March 2015

  • Kim, Daehwan; Langmead, Ben; Salzberg, Steven L.
  • Nature Methods, Vol. 12, Issue 4
  • DOI: 10.1038/nmeth.3317

Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014


Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review
journal, September 2008

  • Taherzadeh, Mohammad; Karimi, Keikhosro
  • International Journal of Molecular Sciences, Vol. 9, Issue 9, p. 1621-1651
  • DOI: 10.3390/ijms9091621

Determination of porosity of lignocellulosic biomass before and after pretreatment by using Simons’ stain and NMR techniques
journal, September 2013


An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock
journal, December 2016