Novel carbohydrate binding modules in the surface anchored α‐amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut

Cockburn, Darrell W.; Suh, Carolyn; Medina, Krizia Perez; Duvall, Rebecca M.; Wawrzak, Zdzislaw; Henrissat, Bernard; Koropatkin, Nicole M.

doi:10.1111/mmi.13881

Novel carbohydrate binding modules in the surface anchored α‐amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut

Journal Article · Thu Nov 30 23:00:00 EST 2017 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.13881· OSTI ID:1410833

Cockburn, Darrell W. ^[1]; Suh, Carolyn ^[2]; Medina, Krizia Perez ^[2]; Duvall, Rebecca M. ^[2]; Wawrzak, Zdzislaw ^[3]; Henrissat, Bernard ^[4]; ^[2]

Department of Microbiology and Immunology University of Michigan Medical School Ann Arbor MI 48109 USA, Department of Food Science Pennsylvania State University, University Park PA 16802 USA
Department of Microbiology and Immunology University of Michigan Medical School Ann Arbor MI 48109 USA
Life Sciences Collaborative Access Team (LS‐CAT), Advanced Photon Source Argonne National Laboratory, 9700 S. Cass Avenue Argonne IL 60439 USA
Architecture et Fonction des Macromolécules Biologiques, CNRS, Aix‐Marseille University Marseille F‐13288 France, Institut National de la Recherche Agronomique, USC1408 Architecture et Fonction des Macromolécules Biologiques Marseille F‐13288 France, Department of Biological Sciences King Abdulaziz University Jeddah 21589 Saudi Arabia

Summary

Gut bacteria recognize accessible glycan substrates within a complex environment. Carbohydrate binding modules (CBMs) of cell surface glycoside hydrolases often drive binding to the target substrate. Eubacterium rectale , an important butyrate‐producing organism in the gut, consumes a limited range of substrates, including starch. Host consumption of resistant starch increases the abundance of E. rectale in the intestine, likely because it successfully captures the products of resistant starch degradation by other bacteria. Here, we demonstrate that the cell wall anchored starch‐degrading α‐amylase, Amy13K of E. rectale harbors five CBMs that all target starch with differing specificities. Intriguingly these CBMs efficiently bind to both regular and high amylose corn starch (a type of resistant starch), but have almost no affinity for potato starch (another type of resistant starch). Removal of these CBMs from Amy13K reduces the activity level of the enzyme toward corn starches by ∼40‐fold, down to the level of activity toward potato starch, suggesting that the CBMs facilitate activity on corn starch and allow its utilization in vivo . The specificity of the Amy13K CBMs provides a molecular rationale for why E. rectale is able to only use certain starch types without the aid of other organisms.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; AC02-06CH11357

OSTI ID:: 1410833

Alternate ID(s):: OSTI ID: 1419064

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 2 Vol. 107; ISSN 0950-382X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: FAO

Language:: English

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