Bifiguratus adelaidae, gen. et sp. nov., a new member of Mucoromycotina in endophytic and soil-dwelling habitats

Torres-Cruz, Terry J.; Billingsley Tobias, Terri L.; Almatruk, Maryam; Hesse, Cedar N.; Kuske, Cheryl R.; Desirò, Alessandro; Benucci, Gian Maria Niccolo; Bonito, Gregory; Stajich, Jason E.; Dunlap, Christopher; Arnold, A. Elizabeth; Porras-Alfaro, Andrea

doi:10.1080/00275514.2017.1364958

Title: Bifiguratus adelaidae, gen. et sp. nov., a new member of Mucoromycotina in endophytic and soil-dwelling habitats

Journal Article · Tue Aug 08 00:00:00 EDT 2017 · Mycologia

DOI:https://doi.org/10.1080/00275514.2017.1364958· OSTI ID:1409768

^[1]; Billingsley Tobias, Terri L. ^[1]; Almatruk, Maryam ^[1]; Hesse, Cedar N. ^[2]; Kuske, Cheryl R. ^[2]; Desirò, Alessandro ^[3]; Benucci, Gian Maria Niccolo ^[3]; Bonito, Gregory ^[3];

^[4]; Dunlap, Christopher ^[5]; Arnold, A. Elizabeth ^[6];

^[1]

Western Illinois Univ., Macomb, IL (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Michigan State Univ., East Lansing, MI (United States)
Univ. of California, Riverside, CA (United States)
USDA Agricultural Research Service (ARS), Peoria, IL (United States). National Center for Agricultural Utilization Research
Univ. of Arizona, Tucson, AZ (United States)

Illumina amplicon sequencing of soil in a temperate pine forest in the southeastern United States detected an abundant, nitrogen (N)-responsive fungal genotype of unknown phylogenetic affiliation. Two isolates with ribosomal sequences consistent with that genotype were subsequently obtained. Examination of records in GenBank revealed that a genetically similar fungus had been isolated previously as an endophyte of moss in a pine forest in the southwestern United States. The three isolates were characterized using morphological, genomic, and multilocus molecular data (18S, internal transcribed spacer [ITS], and 28S rRNA sequences). Phylogenetic and maximum likelihood phylogenomic reconstructions revealed that the taxon represents a novel lineage in Mucoromycotina, only preceded by Calcarisporiella, the earliest diverging lineage in the subphylum. Sequences for the novel taxon are frequently detected in environmental sequencing studies, and it is currently part of UNITE’s dynamic list of most wanted fungi. The fungus is dimorphic, grows best at room temperature, and is associated with a wide variety of bacteria. In this paper, a new monotypic genus, Bifiguratus, is proposed, typified by Bifiguratus adelaidae.

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Research Organization:: Western Illinois Univ., Macomb, IL (United States); Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); USDA Agricultural Research Service (ARS), Peoria, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Inst. of Health (NIH) (United States); Western Illinois Univ. (United States); Dept. of Agriculture (USDA)

Grant/Contract Number:: AC52-06NA25396; DEB-0640996; DEB-0640956; DEB-1441715; DBI-1429826; S10-OD016290

OSTI ID:: 1409768

Report Number(s):: LA-UR-17-22326

Journal Information:: Mycologia, Vol. 109, Issue 3; ISSN 0027-5514

Publisher:: Taylor & FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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Title: Bifiguratus adelaidae, gen. et sp. nov., a new member of Mucoromycotina in endophytic and soil-dwelling habitats

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