Taxonomic and Functional Diversity of a Quercus pyrenaica Willd. Rhizospheric Microbiome in the Mediterranean Mountains

Cobo-Díaz, Jose F.; Fernández-González, Antonio J.; Villadas, Pablo J.; Toro, Nicolás; Tringe, Susannah G.; Fernández-López, Manuel

doi:10.3390/f8100390

Taxonomic and Functional Diversity of a Quercus pyrenaica Willd. Rhizospheric Microbiome in the Mediterranean Mountains

Journal Article · Thu Oct 12 00:00:00 EDT 2017 · Forests

DOI:https://doi.org/10.3390/f8100390· OSTI ID:1408477

Cobo-Díaz, Jose F. ^[1]; Fernández-González, Antonio J. ^[1]; Villadas, Pablo J. ^[1]; Toro, Nicolás ^[1]; Tringe, Susannah G. ^[2]; Fernández-López, Manuel ^[1]

Consejo Superior de Investigaciones Cientificas, Granada (Spain)
DOE Joint Genome Institute, Walnut Creek, CA (United States)

Altitude significantly affects vegetation growth and distribution, including the developmental stages of a forest. We used shotgun Illumina sequencing to analyze microbial community composition and functional potential in melojo-oak (Quercus pyrenaica Willd.) rhizospheric soil for three different development stages along an altitudinal gradient: (a) a low altitude, non-optimal site for forest maintenance; (b) an intermediate altitude, optimal site for a forest; and (c) a high altitude, expansion site with isolated trees but without a real forest canopy. We observed that, at each altitude, the same microbial taxa appear both in the taxonomic analysis of the whole metagenome and in the functional analysis of the methane, sulfur and nitrogen metabolisms. Although there were no major differences at the functional level, there were significant differences in the abundance of each taxon at the phylogenetic level between the rhizospheres of the forest (low and intermediate altitudes) and the expansion site. Proteobacteria and Actinobacteria were the most differentially abundant phyla in forest soils compared to the expansion site rhizosphere. Moreover, Verrucomicrobia, Bacteroidetes and Nitrospirae phyla were more highly represented in the non-forest rhizosphere. Our study suggests that rhizospheric microbial communities of the same tree species may be affected by development stage and forest canopy cover via changes in soil pH and the C/N ratio.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1408477

Journal Information:: Forests, Journal Name: Forests Journal Issue: 10 Vol. 8; ISSN 1999-4907

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Metabarcoding reveals that rhizospheric microbiota of Quercus pyrenaica is composed by a relatively small number of bacterial taxa highly abundant Lasa, Ana V.; Fernández-González, Antonio J.; Villadas, Pablo J. Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-018-38123-z	journal	February 2019
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59 BASIC BIOLOGICAL SCIENCES
Mediterranean forests
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Taxonomic and Functional Diversity of a Quercus pyrenaica Willd. Rhizospheric Microbiome in the Mediterranean Mountains

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