Regulation of Nitrite Stress Response in Desulfovibrio vulgaris Hildenborough, a Model Sulfate-Reducing Bacterium

Rajeev, Lara; Chen, Amy; Kazakov, Alexey E.; Luning, Eric G.; Zane, Grant M.; Novichkov, Pavel S.; Wall, Judy D.; Mukhopadhyay, Aindrila

doi:10.1128/JB.00319-15

Regulation of Nitrite Stress Response in Desulfovibrio vulgaris Hildenborough, a Model Sulfate-Reducing Bacterium

Journal Article · Mon Aug 17 00:00:00 EDT 2015 · Journal of Bacteriology

DOI:https://doi.org/10.1128/JB.00319-15· OSTI ID:1407286

Rajeev, Lara ^[1]; Chen, Amy ^[1]; Kazakov, Alexey E. ^[1]; Luning, Eric G. ^[1]; Zane, Grant M. ^[2]; Novichkov, Pavel S. ^[1]; Wall, Judy D. ^[2]; Mukhopadhyay, Aindrila ^[1]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
Univ. of Missouri, Columbia, MO (United States). Dept. of Biochemistry

Sulfate-reducing bacteria (SRB) are sensitive to low concentrations of nitrite, and nitrite has been used to control SRB-related biofouling in oil fields. Desulfovibrio vulgaris Hildenborough, a model SRB, carries a cytochrome c-type nitrite reductase (nrfHA) that confers resistance to low concentrations of nitrite. The regulation of this nitrite reductase has not been directly examined to date. In this study, we show that DVU0621 (NrfR), a sigma54-dependent two-component system response regulator, is the positive regulator for this operon. NrfR activates the expression of the nrfHA operon in response to nitrite stress. We also show that nrfR is needed for fitness at low cell densities in the presence of nitrite because inactivation of nrfR affects the rate of nitrite reduction. We also predict and validate the binding sites for NrfR upstream of the nrfHA operon using purified NrfR in gel shift assays. Here we discuss possible roles for NrfR in regulating nitrate reductase genes in nitrate-utilizing Desulfovibrio spp. The NrfA nitrite reductase is prevalent across several bacterial phyla and required for dissimilatory nitrite reduction. However, regulation of the nrfA gene has been studied in only a few nitrate-utilizing bacteria. Here, we show that in D. vulgaris, a bacterium that does not respire nitrate, the expression of nrfHA is induced by NrfR upon nitrite stress. This is the first report of regulation of nrfA by a sigma54-dependent two-component system. Finally, our study increases our knowledge of nitrite stress responses and possibly of the regulation of nitrate reduction in SRB.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1407286

Journal Information:: Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 21 Vol. 197; ISSN 0021-9193

Publisher:: American Society for Microbiology

Country of Publication:: United States

Language:: English

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