Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx

Romano, Christine A.; Zhou, Mowei; Song, Yang; Wysocki, Vicki H.; Dohnalkova, Alice C.; Kovarik, Libor; Pasa-Tolic, Ljiljana; Tebo, Bradley M.

doi:10.1038/s41467-017-00896-8

Title: Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx

Journal Article · Fri Sep 29 00:00:00 EDT 2017 · Nature Communications

DOI:https://doi.org/10.1038/s41467-017-00896-8· OSTI ID:1406743

^[1];

^[2]; Song, Yang ^[3]; Wysocki, Vicki H. ^[3]; Dohnalkova, Alice C. ^[2]; Kovarik, Libor ^[2]; Pasa-Tolic, Ljiljana ^[2]; Tebo, Bradley M. ^[1]

Oregon Health & Science Univ., Portland, OR (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
The Ohio State Univ., Columbus, OH (United States)

Bacteria that produce Mn oxides are extraordinarily skilled engineers of nanomaterials that contribute significantly to global biogeochemical cycles. Their enzyme-based reaction mechanisms may be genetically tailored for environmental remediation applications or bioenergy production. However, significant challenges exist for structural characterization of the enzymes responsible for biomineralization. The active Mn oxidase, Mnx, in Bacillus sp. PL-12 is a complex composed of a multicopper oxidase (MCO), MnxG, and two accessory proteins MnxE and MnxF. MnxG shares sequence similarity with other, structurally characterized MCOs. However, MnxE and MnxF have no similarity to any characterized proteins. The ~200 kDa complex has been recalcitrant to crystallization, so its structure is unknown. In this study, native mass spectrometry defines the subunit topology and copper binding of the Mnx complex, while high resolution electron microscopy visualizes the protein and nascent Mn oxide minerals. Furthermore, these data provide critical structural information for conceptualizing how Mnx produces nanoparticulate Mn oxides.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1406743

Report Number(s):: PNNL-SA-124759; 48903; KP1704020

Journal Information:: Nature Communications, Vol. 8, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 51 works

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References (69)

Direction-Specific Interactions Control Crystal Growth by Oriented Attachment Li, D.; Nielsen, M. H.; Lee, J. R. I. Science, Vol. 336, Issue 6084 https://doi.org/10.1126/science.1219643	journal	May 2012
Mn(II) Binding and Subsequent Oxidation by the Multicopper Oxidase MnxG Investigated by Electron Paramagnetic Resonance Spectroscopy Tao, Lizhi; Stich, Troy A.; Butterfield, Cristina N. Journal of the American Chemical Society, Vol. 137, Issue 33 https://doi.org/10.1021/jacs.5b04331	journal	August 2015
Photochemical Water Oxidation by Crystalline Polymorphs of Manganese Oxides: Structural Requirements for Catalysis Robinson, David M.; Go, Yong Bok; Mui, Michelle Journal of the American Chemical Society, Vol. 135, Issue 9 https://doi.org/10.1021/ja310286h	journal	February 2013
Function and molecular evolution of multicopper blue proteins Nakamura, K.; Go, N. Cellular and Molecular Life Sciences https://doi.org/10.1007/s00018-004-5076-5	journal	August 2005
Superoxide dismutases: Ancient enzymes and new insights Miller, Anne-Frances FEBS Letters, Vol. 586, Issue 5 https://doi.org/10.1016/j.febslet.2011.10.048	journal	November 2011
Identification and characterization of a gene cluster involved in manganese oxidation by spores of the marine Bacillus sp. strain SG-1. van Waasbergen, L. G.; Hildebrand, M.; Tebo, B. M. Journal of Bacteriology, Vol. 178, Issue 12 https://doi.org/10.1128/jb.178.12.3517-3530.1996	journal	June 1996
Nonspecific aggregation in native electrokinetic nanoelectrospray ionization Davidson, Kimberly L.; Oberreit, Derek R.; Hogan, Christopher J. International Journal of Mass Spectrometry, Vol. 420 https://doi.org/10.1016/j.ijms.2016.09.013	journal	September 2017
Shall We Dance? How a Multicopper Oxidase Chooses Its Electron Transfer Partner Quintanar, Liliana; Stoj, Christopher; Taylor, Alexander B. ChemInform, Vol. 38, Issue 37 https://doi.org/10.1002/chin.200737266	journal	September 2007
Bacterial enzymes involved in lignin degradation de Gonzalo, Gonzalo; Colpa, Dana I.; Habib, Mohamed H. M. Journal of Biotechnology, Vol. 236 https://doi.org/10.1016/j.jbiotec.2016.08.011	journal	October 2016
Principles of assembly reveal a periodic table of protein complexes Ahnert, S. E.; Marsh, J. A.; Hernandez, H. Science, Vol. 350, Issue 6266 https://doi.org/10.1126/science.aaa2245	journal	December 2015
Computational identification of self-inhibitory peptides from envelope proteins Xu, Yongtao; Rahman, Noorsaadah A. B. D.; Othman, Rozana Proteins: Structure, Function, and Bioinformatics, Vol. 80, Issue 9 https://doi.org/10.1002/prot.24105	journal	June 2012
Metal Sulfide Cluster Complexes and their Biogeochemical Importance in the Environment Luther, George W.; Rickard, David T. Journal of Nanoparticle Research, Vol. 7, Issue 4-5 https://doi.org/10.1007/s11051-005-4272-4	journal	October 2005
Coupled biotic–abiotic Mn(II) oxidation pathway mediates the formation and structural evolution of biogenic Mn oxides Learman, D. R.; Wankel, S. D.; Webb, S. M. Geochimica et Cosmochimica Acta, Vol. 75, Issue 20 https://doi.org/10.1016/j.gca.2011.07.026	journal	October 2011
Ligand binding and unfolding of tryptophan synthase revealed by ion mobility-tandem mass spectrometry employing collision and surface induced dissociation Quintyn, Royston S.; Zhou, Mowei; Dagan, Shai International Journal for Ion Mobility Spectrometry, Vol. 16, Issue 2 https://doi.org/10.1007/s12127-013-0126-4	journal	March 2013
Multicopper oxidase involvement in both Mn(II) and Mn(III) oxidation during bacterial formation of MnO2 Soldatova, Alexandra V.; Butterfield, Cristina; Oyerinde, Oyeyemi F. JBIC Journal of Biological Inorganic Chemistry, Vol. 17, Issue 8 https://doi.org/10.1007/s00775-012-0928-6	journal	August 2012
Heterologous Expression and Characterization of the Manganese-Oxidizing Protein from Erythrobacter sp. Strain SD21 Nakama, Katherine; Medina, Michael; Lien, Ahn Applied and Environmental Microbiology, Vol. 80, Issue 21 https://doi.org/10.1128/aem.01873-14	journal	August 2014
Surface Induced Dissociation: Dissecting Noncovalent Protein Complexes in the Gas phase Zhou, Mowei; Wysocki, Vicki H. Accounts of Chemical Research, Vol. 47, Issue 4 https://doi.org/10.1021/ar400223t	journal	February 2014
Manganous Phosphate Acts as a Superoxide Dismutase Barnese, Kevin; Gralla, Edith B.; Cabelli, Diane E. Journal of the American Chemical Society, Vol. 130, Issue 14 https://doi.org/10.1021/ja710162n	journal	April 2008
Multicopper oxidases: a workshop on copper coordination chemistry, electron transfer, and metallophysiology Kosman, Daniel J. JBIC Journal of Biological Inorganic Chemistry, Vol. 15, Issue 1 https://doi.org/10.1007/s00775-009-0590-9	journal	October 2009
Mn(II) Oxidation Is Catalyzed by Heme Peroxidases in "Aurantimonas manganoxydans" Strain SI85-9A1 and Erythrobacter sp. Strain SD-21 Anderson, C. R.; Johnson, H. A.; Caputo, N. Applied and Environmental Microbiology, Vol. 75, Issue 12 https://doi.org/10.1128/aem.02890-08	journal	May 2009
Water-oxidation catalysis by manganese in a geochemical-like cycle Hocking, Rosalie K.; Brimblecombe, Robin; Chang, Lan-Yun Nature Chemistry, Vol. 3, Issue 6 https://doi.org/10.1038/nchem.1049	journal	May 2011
The I-TASSER Suite: protein structure and function prediction Yang, Jianyi; Yan, Renxiang; Roy, Ambrish Nature Methods, Vol. 12, Issue 1 https://doi.org/10.1038/nmeth.3213	journal	December 2014
Inter-relationships of MnO2 precipitation, siderophore–Mn(III) complex formation, siderophore degradation, and iron limitation in Mn(II)-oxidizing bacterial cultures Parker, Dorothy L.; Morita, Takami; Mozafarzadeh, Mylene L. Geochimica et Cosmochimica Acta, Vol. 71, Issue 23 https://doi.org/10.1016/j.gca.2007.03.042	journal	December 2007
The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium Imlay, James A. Nature Reviews Microbiology, Vol. 11, Issue 7 https://doi.org/10.1038/nrmicro3032	journal	May 2013
Direct Identification of a Bacterial Manganese(II) Oxidase, the Multicopper Oxidase MnxG, from Spores of Several Different Marine Bacillus Species Dick, G. J.; Torpey, J. W.; Beveridge, T. J. Applied and Environmental Microbiology, Vol. 74, Issue 5 https://doi.org/10.1128/aem.01240-07	journal	December 2007
Mineralization in Ferritin: An Efficient Means of Iron Storage Chasteen, N. Dennis; Harrison, Pauline M. Journal of Structural Biology, Vol. 126, Issue 3 https://doi.org/10.1006/jsbi.1999.4118	journal	June 1999
NIH Image to ImageJ: 25 years of image analysis Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W. Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2089	journal	June 2012
Mn(II,III) oxidation and MnO2 mineralization by an expressed bacterial multicopper oxidase Butterfield, C. N.; Soldatova, A. V.; Lee, S. -W. Proceedings of the National Academy of Sciences, Vol. 110, Issue 29 https://doi.org/10.1073/pnas.1303677110	journal	July 2013
Fungal laccases – occurrence and properties Baldrian, Petr FEMS Microbiology Reviews, Vol. 30, Issue 2 https://doi.org/10.1111/j.1574-4976.2005.00010.x	journal	March 2006
Shall We Dance? How A Multicopper Oxidase Chooses Its Electron Transfer Partner Quintanar, Liliana; Stoj, Christopher; Taylor, Alexander B. Accounts of Chemical Research, Vol. 40, Issue 6 https://doi.org/10.1021/ar600051a	journal	June 2007
Multicopper Oxidases and Oxygenases Solomon, Edward I.; Sundaram, Uma M.; Machonkin, Timothy E. Chemical Reviews, Vol. 96, Issue 7 https://doi.org/10.1021/cr950046o	journal	January 1996
Surface-Induced Dissociation of Homotetramers with D2 Symmetry Yields their Assembly Pathways and Characterizes the Effect of Ligand Binding Quintyn, Royston S.; Yan, Jing; Wysocki, Vicki H. Chemistry & Biology, Vol. 22, Issue 5 https://doi.org/10.1016/j.chembiol.2015.03.019	journal	May 2015
Identification of a Third Mn(II) Oxidase Enzyme in Pseudomonas putida GB-1 Geszvain, Kati; Smesrud, Logan; Tebo, Bradley M. Applied and Environmental Microbiology, Vol. 82, Issue 13 https://doi.org/10.1128/aem.00046-16	journal	April 2016
Copper incorporation into recombinant CotA laccase from Bacillus subtilis: characterization of fully copper loaded enzymes Durão, Paulo; Chen, Zhenjia; Fernandes, André T. JBIC Journal of Biological Inorganic Chemistry, Vol. 13, Issue 2 https://doi.org/10.1007/s00775-007-0312-0	journal	October 2007
Non-heme manganese catalase – The ‘other’ catalase Whittaker, James W. Archives of Biochemistry and Biophysics, Vol. 525, Issue 2 https://doi.org/10.1016/j.abb.2011.12.008	journal	September 2012
Collision-Induced Dissociation of Electrosprayed Protein Complexes: An All-Atom Molecular Dynamics Model with Mobile Protons Popa, Vlad; Trecroce, Danielle A.; McAllister, Robert G. The Journal of Physical Chemistry B, Vol. 120, Issue 23 https://doi.org/10.1021/acs.jpcb.6b03035	journal	May 2016
Multicopper manganese oxidase accessory proteins bind Cu and heme Butterfield, Cristina N.; Tao, Lizhi; Chacón, Kelly N. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1854, Issue 12 https://doi.org/10.1016/j.bbapap.2015.08.012	journal	December 2015
The catalytic center of ferritin regulates iron storage via Fe(II)-Fe(III) displacement Honarmand Ebrahimi, Kourosh; Bill, Eckhard; Hagedoorn, Peter-Leon Nature Chemical Biology, Vol. 8, Issue 11 https://doi.org/10.1038/nchembio.1071	journal	September 2012
Protein Complexes in the Gas Phase: Technology for Structural Genomics and Proteomics Benesch, Justin L. P.; Ruotolo, Brandon T.; Simmons, Douglas A. Chemical Reviews, Vol. 107, Issue 8 https://doi.org/10.1021/cr068289b	journal	August 2007
NMR reveals pathway for ferric mineral precursors to the central cavity of ferritin Turano, P.; Lalli, D.; Felli, I. C. Proceedings of the National Academy of Sciences, Vol. 107, Issue 2 https://doi.org/10.1073/pnas.0908082106	journal	December 2009
Extracellular haem peroxidases mediate Mn(II) oxidation in a marine R oseobacter bacterium via superoxide production : Peroxidases mediate superoxide-based Mn oxidation Andeer, Peter F.; Learman, Deric R.; McIlvin, Matt Environmental Microbiology, Vol. 17, Issue 10 https://doi.org/10.1111/1462-2920.12893	journal	June 2015
Investigation of intact protein complexes by mass spectrometry Heck, Albert J. R.; van den Heuvel, Robert H. H. Mass Spectrometry Reviews, Vol. 23, Issue 5 https://doi.org/10.1002/mas.10081	journal	January 2004
Localization of Mn(II)-oxidizing activity and the putative multicopper oxidase, MnxG, to the exosporium of the marine Bacillus sp. strain SG-1 Francis, Chris; Casciotti, Karen; Tebo, Bradley Archives of Microbiology, Vol. 178, Issue 6 https://doi.org/10.1007/s00203-002-0472-9	journal	December 2002
Manganese binding and oxidation by spores of a marine bacillus. Rosson, R. A.; Nealson, K. H. Journal of Bacteriology, Vol. 151, Issue 2 https://doi.org/10.1128/jb.151.2.1027-1034.1982	journal	January 1982
Manganese (Mn) Oxidation Increases Intracellular Mn in Pseudomonas putida GB-1 Banh, Andy; Chavez, Valarie; Doi, Julia PLoS ONE, Vol. 8, Issue 10 https://doi.org/10.1371/journal.pone.0077835	journal	October 2013
Constraints on superoxide mediated formation of manganese oxides Learman, Deric R.; Voelker, Bettina M.; Madden, Andrew S. Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00262	journal	January 2013
Function and molecular evolution of multicopper blue proteins Nakamura, K.; Go, N. Cellular and Molecular Life Sciences, Vol. 62, Issue 18 https://doi.org/10.1007/s00018-004-5076-x	journal	August 2005
ZDOCK server: interactive docking prediction of protein-protein complexes and symmetric multimers Pierce, B. G.; Wiehe, K.; Hwang, H. Bioinformatics, Vol. 30, Issue 12 https://doi.org/10.1093/bioinformatics/btu097	journal	February 2014
CotA, a Multicopper Oxidase from Bacillus pumilus WH4, Exhibits Manganese-Oxidase Activity Su, Jianmei; Bao, Peng; Bai, Tenglong PLoS ONE, Vol. 8, Issue 4 https://doi.org/10.1371/journal.pone.0060573	journal	April 2013
Identification of catalytically important amino acids in human ceruloplasmin by site-directed mutagenesis Brown, Mark A.; Stenberg, Leisa M.; Mauk, A. Grant FEBS Letters, Vol. 520, Issue 1-3 https://doi.org/10.1016/s0014-5793(02)02652-2	journal	May 2002
Manganese oxidation by Leptothrix discophora. Boogerd, F. C.; de Vrind, J. P. Journal of Bacteriology, Vol. 169, Issue 2 https://doi.org/10.1128/jb.169.2.489-494.1987	journal	February 1987
Ab initio protein structure assembly using continuous structure fragments and optimized knowledge-based force field Xu, Dong; Zhang, Yang Proteins: Structure, Function, and Bioinformatics https://doi.org/10.1002/prot.24065	journal	January 2012
Protein Oxidation Implicated as the Primary Determinant of Bacterial Radioresistance Daly, Michael J.; Gaidamakova, Elena K.; Matrosova, Vera Y. PLoS Biology, Vol. 5, Issue 4, Article No. e92 https://doi.org/10.1371/journal.pbio.0050092	journal	March 2007
Manganese(II) adsorption and oxidation by whole cells and a membrane fraction of Pedomicrobium sp. ACM 3067 Larsen, E. I.; Sly, Lindsay I.; McEwan, Alastair G. Archives of Microbiology, Vol. 171, Issue 4 https://doi.org/10.1007/s002030050708	journal	March 1999
Enzymatic Manganese(II) Oxidation by Metabolically Dormant Spores of Diverse Bacillus Species Francis, C. A.; Tebo, B. M. Applied and Environmental Microbiology, Vol. 68, Issue 2 https://doi.org/10.1128/aem.68.2.874-880.2002	journal	February 2002
Nature of Activated Manganese Oxide for Oxygen Evolution Huynh, Michael; Shi, Chenyang; Billinge, Simon J. L. Journal of the American Chemical Society, Vol. 137, Issue 47 https://doi.org/10.1021/jacs.5b06382	journal	November 2015
Partial purification and characterization of manganese-oxidizing factors of Pseudomonas fluorescens GB-1. Okazaki, M.; Sugita, T.; Shimizu, M. Applied and environmental microbiology, Vol. 63, Issue 12 https://doi.org/10.1128/aem.63.12.4793-4799.1997	journal	January 1997
Protein Complexes in the Gas Phase: Technology for Structural Genomics and Proteomics Benesch, Justin L. P.; Ruotolo, Brandon T.; Simmons, Douglas A. ChemInform, Vol. 38, Issue 47 https://doi.org/10.1002/chin.200747245	journal	November 2007
Evidence for the presence of Mn(III) intermediates in the bacterial oxidation of Mn(II) Webb, S. M.; Dick, G. J.; Bargar, J. R. Proceedings of the National Academy of Sciences, Vol. 102, Issue 15 https://doi.org/10.1073/pnas.0409119102	journal	March 2005
Drilling accurate nanopores for biosensors by energetic multi-wall carbon nanotubes: a molecular dynamics investigation Li, Changsheng; Wang, Zilin; Ma, Lei Journal of Molecular Modeling, Vol. 28, Issue 10 https://doi.org/10.1007/s00894-022-05276-8	journal	September 2022
Origin of the Breit-Wigner-Fano lineshape of the tangential G -band feature of metallic carbon nanotubes Brown, S. D. M.; Jorio, A.; Corio, P. Physical Review B, Vol. 63, Issue 15 https://doi.org/10.1103/PhysRevB.63.155414	journal	March 2001
Diastereoselective Carbonyl Allylation with Simple Olefins Enabled by Palladium Complex-Catalyzed C–H Oxidative Borylation Tao, Zhong-Lin; Li, Xing-Han; Han, Zhi-Yong Journal of the American Chemical Society, Vol. 137, Issue 12 https://doi.org/10.1021/jacs.5b00507	journal	March 2015
Substrate specificity and copper loading of the manganese-oxidizing multicopper oxidase Mnx from Bacillus sp. PL-12 Butterfield, Cristina N.; Tebo, Bradley M. Metallomics, Vol. 9, Issue 2 https://doi.org/10.1039/c6mt00239k	journal	January 2017
Manganese-oxidizing photosynthesis before the rise of cyanobacteria Johnson, J. E.; Webb, S. M.; Thomas, K. Proceedings of the National Academy of Sciences, Vol. 110, Issue 28 https://doi.org/10.1073/pnas.1305530110	journal	June 2013
Manganese(II)-Oxidizing Bacillus Spores in Guaymas Basin Hydrothermal Sediments and Plumes Dick, G. J.; Lee, Y. E.; Tebo, B. M. Applied and Environmental Microbiology, Vol. 72, Issue 5 https://doi.org/10.1128/aem.72.5.3184-3190.2006	journal	May 2006
Manganese, superoxide dismutase, and oxygen tolerance in some lactic acid bacteria. Archibald, F. S.; Fridovich, I. Journal of Bacteriology, Vol. 146, Issue 3 https://doi.org/10.1128/jb.146.3.928-936.1981	journal	January 1981
Manganese and Defenses against Oxygen Toxicity in Lactobacillus plantarum Archibald, Frederick S.; Fridovich, Irwin Journal of Bacteriology, Vol. 145, Issue 1 https://doi.org/10.1128/jb.145.1.442-451.1981	journal	January 1981
BIOGENIC MANGANESE OXIDES: Properties and Mechanisms of Formation Tebo, Bradley M.; Bargar, John R.; Clement, Brian G. Annual Review of Earth and Planetary Sciences, Vol. 32, Issue 1 https://doi.org/10.1146/annurev.earth.32.101802.120213	journal	May 2004
Towards a mechanistic understanding of carbon stabilization in manganese oxides Johnson, Karen; Purvis, Graham; Lopez-Capel, Elisa Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms8628	journal	July 2015

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