Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase

Prakash, Aishwarya; Moharana, Kedar; Wallace, Susan S.; Doublié, Sylvie

doi:10.1093/nar/gkw1282

Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase

Journal Article · Sun Dec 18 23:00:00 EST 2016 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkw1282· OSTI ID:1404951

Prakash, Aishwarya ^[1]; Moharana, Kedar ^[2]; Wallace, Susan S. ^[2]; Doublié, Sylvie ^[2]

Univ. of South Alabama, Mobile, AL (United States)
Univ. of Vermont, Burlington, VT (United States). The Markey Center for Molecular Genetics

The base excision repair (BER) pathway repairs oxidized lesions in the DNA that result from reactive oxygen species generated in cells. If left unrepaired, these damaged DNA bases can disrupt cellular processes such as replication. NEIL1 is one of the 11 human DNA glycosylases that catalyze the first step of the BER pathway, i.e. recognition and excision of DNA lesions. NEIL1 interacts with essential replication proteins such as the ring-shaped homotrimeric proliferating cellular nuclear antigen (PCNA). We isolated a complex formed between NEIL1 and PCNA (±DNA) using size exclusion chromatography (SEC). This interaction was confirmed using native gel electrophoresis and mass spectrometry. Stokes radii measured by SEC hinted that PCNA in complex with NEIL1 (±DNA) was no longer a trimer. Height measurements and images obtained by atomic force microscopy also demonstrated the dissociation of the PCNA homotrimer in the presence of NEIL1 and DNA, while small-angle X-ray scattering analysis confirmed the NEIL1 mediated PCNA trimer dissociation and formation of a 1:1:1 NEIL1-DNA-PCNA(monomer) complex. Furthermore, ab initio shape reconstruction provides insights into the solution structure of this previously unreported complex. Together, these data point to a potential mechanistic switch between replication and BER.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH)

OSTI ID:: 1404951

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: (5) ; 03, 2017 Vol. 45; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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