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Robust biological nitrogen fixation in a model grass–bacterial association

Journal Article · · The Plant Journal
DOI:https://doi.org/10.1111/tpj.12777· OSTI ID:1401375
 [1];  [2];  [1];  [3];  [4];  [4];  [2];  [1];  [1];  [1];  [5];  [4]
  1. Department of Biochemistry and Molecular Biology Federal University of Paraná 81531‐980 Curitiba Brazil
  2. Department of Food Science and Technology Federal University of Santa Catarina 88034‐001 Florianópolis Brazil
  3. Department of Environmental Biology State University of New York College of Environmental Science and Forestry Syracuse NY 13210 USA
  4. Biological Environmental and Climate Sciences Department Brookhaven National Laboratory Building 901 Upton NY 11973 USA
  5. Divisions of Plant Science and Biochemistry C. S. Bond Life Science Center University of Missouri Columbia MO 65211 USA
Summary

Nitrogen‐fixing rhizobacteria can promote plant growth; however, it is controversial whether biological nitrogen fixation ( BNF ) from associative interaction contributes to growth promotion. The roots of Setaria viridis , a model C 4 grass, were effectively colonized by bacterial inoculants resulting in a significant enhancement of growth. Nitrogen‐13 tracer studies provided direct evidence for tracer uptake by the host plant and incorporation into protein. Indeed, plants showed robust growth under nitrogen‐limiting conditions when inoculated with an ammonium‐excreting strain of Azospirillum brasilense . 11 C‐labeling experiments showed that patterns in central carbon metabolism and resource allocation exhibited by nitrogen‐starved plants were largely reversed by bacterial inoculation, such that they resembled plants grown under nitrogen‐sufficient conditions. Adoption of S. viridis as a model should promote research into the mechanisms of associative nitrogen fixation with the ultimate goal of greater adoption of BNF for sustainable crop production.

Sponsoring Organization:
USDOE
OSTI ID:
1401375
Alternate ID(s):
OSTI ID: 1184513
Journal Information:
The Plant Journal, Journal Name: The Plant Journal Journal Issue: 6 Vol. 81; ISSN 0960-7412
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
United Kingdom
Language:
English

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