Methods for suspension culture, protoplast extraction, and transformation of high‐biomass yielding perennial grass Arundo donax

Pigna, Gaia; Dhillon, Taniya; Dlugosz, Elizabeth M.; Yuan, Joshua S.; Gorman, Connor; Morandini, Piero; Lenaghan, Scott C.; Stewart, Jr., C. Neal

doi:10.1002/biot.201600486

Title: Methods for suspension culture, protoplast extraction, and transformation of high‐biomass yielding perennial grass Arundo donax

Journal Article · Mon Nov 28 00:00:00 EST 2016 · Biotechnology Journal

DOI:https://doi.org/10.1002/biot.201600486· OSTI ID:1401244

Pigna, Gaia ^[1]; Dhillon, Taniya ^[2]; Dlugosz, Elizabeth M. ^[2]; Yuan, Joshua S. ^[3]; Gorman, Connor ^[3]; Morandini, Piero ^[4]; Lenaghan, Scott C. ^[5]; Stewart, Jr., C. Neal ^[2]

Department of Plant Sciences University of Tennessee Knoxville Tennessee USA, Department of Biosciences University of Milan Milano Italy
Department of Plant Sciences University of Tennessee Knoxville Tennessee USA
Department of Plant Pathology and Microbiology Texas A&,M University College Station Texas USA
Department of Biosciences University of Milan Milano Italy, National Research Council Institute of Biophysics Milano Italy
Department of Food Science University of Tennessee Knoxville Tennessee USA, Department of Mechanical, Aerospace, and Biomedical Engineering University of Tennessee Knoxville Tennessee USA

Abstract Arundo donax L. is a promising biofuel feedstock in the Mediterranean region. Despite considerable interest in its genetic improvement, Arundo tissue culture and transformation remains arduous. The authors developed methodologies for cell‐ and tissue culture and genetic engineering in Arundo . A media screen was conducted, and a suspension culture was established using callus induced from stem axillary bud explants. DBAP medium, containing 9 µM 2,4‐D and 4.4 µM BAP, was found to be the most effective medium among those tested for inducing cell suspension cultures, which resulted in a five‐fold increase in tissue mass over 14 days. In contrast, CIM medium containing 13 µM 2,4‐D, resulted in just a 1.4‐fold increase in mass over the same period. Optimized suspension cultures were superior to previously‐described solidified medium‐based callus culture methods for tissue mass increase. Suspension cultures proved to be very effective for subsequent protoplast isolation. Protoplast electroporation resulted in a 3.3 ± 1.5% transformation efficiency. A dual fluorescent reporter gene vector enabled the direct comparison of the CAMV 35S promoter with the switchgrass ubi2 promoter in single cells of Arundo . The switchgrass ubi2 promoter resulted in noticeably higher reporter gene expression compared with that conferred by the 35S promoter in Arundo .

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Sponsoring Organization:: USDOE

OSTI ID:: 1401244

Journal Information:: Biotechnology Journal, Journal Name: Biotechnology Journal Vol. 11 Journal Issue: 12; ISSN 1860-6768

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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Cited by: 4 works

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