Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol

Pereira, Brian; Zhang, Haoran; De Mey, Marjan; Lim, Chin Giaw; Li, Zheng‐Jun; Stephanopoulos, Gregory

doi:10.1002/bit.25717

Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol

Journal Article · Fri Sep 18 04:00:00 EDT 2015 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.25717· OSTI ID:1401151

Pereira, Brian ^[1]; Zhang, Haoran ^[1]; De Mey, Marjan ^[2]; Lim, Chin Giaw ^[1]; Li, Zheng‐Jun ^[3]; Stephanopoulos, Gregory ^[1]

Department of Chemical Engineering Massachusetts Institute of Technology Cambridge Massachusetts 02139
Department of Chemical Engineering Massachusetts Institute of Technology Cambridge Massachusetts 02139, Center for Industrial Biotechnology and Biocatalysis, Department for Biochemical and Microbial Technology Ghent University Ghent Belgium
Department of Chemical Engineering Massachusetts Institute of Technology Cambridge Massachusetts 02139, Beijing Key Laboratory of Bioprocess, College of Life Science and Technology Beijing University of Chemical Technology Beijing P. R. China

ABSTRACT

Ethylene glycol (EG) is an important commodity chemical with broad industrial applications. It is presently produced from petroleum or natural gas feedstocks in processes requiring consumption of significant quantities of non‐renewable resources. Here, we report a novel pathway for biosynthesis of EG from the renewable sugar glucose in metabolically engineered Escherichia coli . Serine‐to‐EG conversion was first achieved through a pathway comprising serine decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase. Serine provision in E. coli was then enhanced by overexpression of the serine‐biosynthesis pathway. The integration of these two parts into the complete EG‐biosynthesis pathway in E. coli allowed for production of 4.1 g/L EG at a cumulative yield of 0.14 g‐EG/g‐glucose, establishing a foundation for a promising biotechnology. Biotechnol. Bioeng. 2016;113: 376–383. © 2015 Wiley Periodicals, Inc.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0008744

OSTI ID:: 1401151

Journal Information:: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 2 Vol. 113; ISSN 0006-3592

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United States

Language:: English

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