Identification, evolution and functional characterization of two Zn CDF‐family transporters of the ectomycorrhizal fungus Suillus luteus

Ruytinx, Joske; Coninx, Laura; Nguyen, Hoai; Smisdom, Nick; Morin, Emmanuelle; Kohler, Annegret; Cuypers, Ann; Colpaert, Jan V.

doi:10.1111/1758-2229.12551

Title: Identification, evolution and functional characterization of two Zn CDF‐family transporters of the ectomycorrhizal fungus Suillus luteus

Journal Article · Tue Jun 27 00:00:00 EDT 2017 · Environmental Microbiology Reports

DOI:https://doi.org/10.1111/1758-2229.12551· OSTI ID:1400856

^[1]; Coninx, Laura ^[1]; Nguyen, Hoai ^[1]; Smisdom, Nick ^[2]; Morin, Emmanuelle ^[3]; Kohler, Annegret ^[3]; Cuypers, Ann ^[1]; Colpaert, Jan V. ^[1]

Centre for Environmental Sciences, Environmental Biology Hasselt University Agoralaan building D Diepenbeek 3590 Belgium
Biomedical Research Institute Hasselt University Agoralaan building C Diepenbeek 3590 Belgium
Institut National de la Recherche Agronomique UMR1136 INRA‐Université de Lorraine Interactions Arbres/Microorganismes, Laboratoire d'Excellence ARBRE Champenoux 54280 France

Summary Two genes, SlZnT1 and SlZnT2 , encoding Cation Diffusion Facilitator (CDF) family transporters were isolated from Suillus luteus mycelium by genome walking. Both gene models are very similar and phylogenetic analysis indicates that they are most likely the result of a recent gene duplication event. Comparative sequence analysis of the deduced proteins predicts them to be Zn transporters. This function was confirmed by functional analysis in yeast for SlZnT1 . SlZnT1 was able to restore growth of the highly Zn sensitive yeast mutant Δzrc1 and localized to the vacuolar membrane. Transformation of Δzrc1 yeast cells with SlZnT1 resulted in an increased accumulation of Zn compared to empty vector transformed Δzrc1 yeast cells and equals Zn accumulation in wild type yeast cells. We were not able to express functional SlZnT2 in yeast. In S. luteus , both SlZnT genes are constitutively expressed whatever the external Zn concentrations. A labile Zn pool was detected in the vacuoles of S. luteus free‐living mycelium. Therefore we conclude that SlZnT1 is indispensable for maintenance of Zn homeostasis by transporting excess Zn into the vacuole.

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Sponsoring Organization:: USDOE

OSTI ID:: 1400856

Journal Information:: Environmental Microbiology Reports, Journal Name: Environmental Microbiology Reports Vol. 9 Journal Issue: 4; ISSN 1758-2229

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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