Cooperative Subunit Refolding of a Light‐Harvesting Protein through a Self‐Chaperone Mechanism

Laos, Alistair J.; Dean, Jacob C.; Toa, Zi S. D.; Wilk, Krystyna E.; Scholes, Gregory D.; Curmi, Paul M. G.; Thordarson, Pall

doi:10.1002/ange.201607921

Cooperative Subunit Refolding of a Light‐Harvesting Protein through a Self‐Chaperone Mechanism

Journal Article · Wed Feb 08 23:00:00 EST 2017 · Angewandte Chemie

DOI:https://doi.org/10.1002/ange.201607921· OSTI ID:1400819

Laos, Alistair J. ^[1]; Dean, Jacob C. ^[2]; Toa, Zi S. D. ^[2]; Wilk, Krystyna E. ^[3]; Scholes, Gregory D. ^[2]; Curmi, Paul M. G. ^[3]; ^[4]

School of Chemistry the Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology The University of New South Wales Sydney 2052 NSW Australia, School of Physics The University of New South Wales Sydney 2052 NSW Australia
Department of Chemistry Princeton University Princeton NJ 08544 USA
School of Physics The University of New South Wales Sydney 2052 NSW Australia
School of Chemistry the Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology The University of New South Wales Sydney 2052 NSW Australia

Abstract

The fold of a protein is encoded by its amino acid sequence, but how complex multimeric proteins fold and assemble into functional quaternary structures remains unclear. Here we show that two structurally different phycobiliproteins refold and reassemble in a cooperative manner from their unfolded polypeptide subunits, without biological chaperones. Refolding was confirmed by ultrafast broadband transient absorption and two‐dimensional electronic spectroscopy to probe internal chromophores as a marker of quaternary structure. Our results demonstrate a cooperative, self‐chaperone refolding mechanism, whereby the β‐subunits independently refold, thereby templating the folding of the α‐subunits, which then chaperone the assembly of the native complex, quantitatively returning all coherences. Our results indicate that subunit self‐chaperoning is a robust mechanism for heteromeric protein folding and assembly that could also be applied in self‐assembled synthetic hierarchical systems.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; SC0001035

OSTI ID:: 1400819

Journal Information:: Angewandte Chemie, Journal Name: Angewandte Chemie Journal Issue: 29 Vol. 129; ISSN 0044-8249

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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