Chlamydomonas as a model for biofuels and bio‐products production

Scranton, Melissa A.; Ostrand, Joseph T.; Fields, Francis J.; Mayfield, Stephen P.

doi:10.1111/tpj.12780

Title: Chlamydomonas as a model for biofuels and bio‐products production

Journal Article · Wed Feb 18 00:00:00 EST 2015 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.12780· OSTI ID:1400689

Scranton, Melissa A. ^[1]; Ostrand, Joseph T. ^[1]; Fields, Francis J. ^[1]; Mayfield, Stephen P. ^[1]

California Center for Algae Biotechnology Division of Biological Sciences University of California San Diego 9500 Gilman Dr La Jolla CA 92093‐0368 USA

Summary Developing renewable energy sources is critical to maintaining the economic growth of the planet while protecting the environment. First generation biofuels focused on food crops like corn and sugarcane for ethanol production, and soybean and palm for biodiesel production. Second generation biofuels based on cellulosic ethanol produced from terrestrial plants, has received extensive funding and recently pilot facilities have been commissioned, but to date output of fuels from these sources has fallen well short of what is needed. Recent research and pilot demonstrations have highlighted the potential of algae as one of the most promising sources of sustainable liquid transportation fuels. Algae have also been established as unique biofactories for industrial, therapeutic, and nutraceutical co‐products. Chlamydomonas reinhardtii 's long established role in the field of basic research in green algae has paved the way for understanding algal metabolism and developing genetic engineering protocols. These tools are now being utilized in C. reinhardtii and in other algal species for the development of strains to maximize biofuels and bio‐products yields from the lab to the field.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: DE‐EE0003373

OSTI ID:: 1400689

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Vol. 82 Journal Issue: 3; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 158 works

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References (99)

Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase Wu-Scharf, D. Science, Vol. 290, Issue 5494 https://doi.org/10.1126/science.290.5494.1159	journal	November 2000
High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA Zhang, Ru; Patena, Weronika; Armbruster, Ute The Plant Cell, Vol. 26, Issue 4 https://doi.org/10.1105/tpc.114.124099	journal	April 2014
Molecular mechanisms of DNA double-strand break repair Kanaar, Roland; Hoeijmakers, Jan H. J.; van Gent, Dik C. Trends in Cell Biology, Vol. 8, Issue 12 https://doi.org/10.1016/S0962-8924(98)01383-X	journal	December 1998
Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol Li, Yantao; Han, Danxiang; Hu, Guongrong Metabolic Engineering, Vol. 12, Issue 4, p. 387-391 https://doi.org/10.1016/j.ymben.2010.02.002	journal	July 2010
Algal biofuels: Challenges and opportunities Leite, Gustavo B.; Abdelaziz, Ahmed E. M.; Hallenbeck, Patrick C. Bioresource Technology, Vol. 145 https://doi.org/10.1016/j.biortech.2013.02.007	journal	October 2013
Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii: Production of therapeutic proteins in algae Rasala, Beth A.; Muto, Machiko; Lee, Philip A. Plant Biotechnology Journal, Vol. 8, Issue 6 https://doi.org/10.1111/j.1467-7652.2010.00503.x	journal	March 2010
Chloroplast Biogenesis of Photosystem II Cores Involves a Series of Assembly-Controlled Steps That Regulate Translation Minai, Limor; Wostrikoff, Katia; Wollman, Francis-André The Plant Cell, Vol. 18, Issue 1 https://doi.org/10.1105/tpc.105.037705	journal	December 2005
Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii. Sodeinde, O. A.; Kindle, K. L. Proceedings of the National Academy of Sciences, Vol. 90, Issue 19 https://doi.org/10.1073/pnas.90.19.9199	journal	October 1993
Microalgae for a macroenergy world Oncel, Suphi S. Renewable and Sustainable Energy Reviews, Vol. 26 https://doi.org/10.1016/j.rser.2013.05.059	journal	October 2013
Algae biofuels: versatility for the future of bioenergy Jones, Carla S.; Mayfield, Stephen P. Current Opinion in Biotechnology, Vol. 23, Issue 3 https://doi.org/10.1016/j.copbio.2011.10.013	journal	June 2012
Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production James, B. D.; Baum, G. N.; Perez, J. https://doi.org/10.2172/1218404	report	September 2009
Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines Gregory, James A.; Topol, Aaron B.; Doerner, David Z. Applied and Environmental Microbiology, Vol. 79, Issue 13 https://doi.org/10.1128/AEM.00714-13	journal	April 2013
The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas Schroda, Michael; Blocker, Dagmar; Beck, Christoph F. The Plant Journal, Vol. 21, Issue 2 https://doi.org/10.1046/j.1365-313x.2000.00652.x	journal	January 2000
Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii Almaraz-Delgado, Alma Lorena; Flores-Uribe, José; Pérez-España, Víctor Hugo AMB Express, Vol. 4, Issue 1 https://doi.org/10.1186/s13568-014-0057-4	journal	August 2014
Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids Couso, I.; Vila, M.; Rodriguez, H. Biotechnology Progress, Vol. 27, Issue 1 https://doi.org/10.1002/btpr.527	journal	January 2011
A rapid live-cell ELISA for characterizing antibodies against cell surface antigens of Chlamydomonas reinhardtii and its use in isolating algae from natural environments with related cell wall components Jiang, Wenzhi; Cossey, Sarah; Rosenberg, Julian N. BMC Plant Biology, Vol. 14, Issue 1 https://doi.org/10.1186/s12870-014-0244-0	journal	September 2014
Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii Melis, Anastasios; Zhang, Liping; Forestier, Marc Plant Physiology, Vol. 122, Issue 1 https://doi.org/10.1104/pp.122.1.127	journal	January 2000
Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii La Russa, M.; Bogen, C.; Uhmeyer, A. Journal of Biotechnology, Vol. 162, Issue 1 https://doi.org/10.1016/j.jbiotec.2012.04.006	journal	November 2012
Synthetic promoters genetic control through cis engineering Venter, Mauritz Trends in Plant Science, Vol. 12, Issue 3, p. 118-124 https://doi.org/10.1016/j.tplants.2007.01.002	journal	March 2007
Fatty acid profiling of Chlamydomonas reinhardtii under nitrogen deprivation James, Gabriel O.; Hocart, Charles H.; Hillier, Warwick Bioresource Technology, Vol. 102, Issue 3 https://doi.org/10.1016/j.biortech.2010.11.051	journal	February 2011
Hydrogen Production by Chlamydomonas reinhardtii Torzillo, Giuseppe; Seibert, Michael Handbook of Microalgal Culture: Applied Phycology and Biotechnology, Second Edition https://doi.org/10.1002/9781118567166.ch22	book	January 2013
Production of anti-cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners: Production of Anti-Cancer Immunotoxins in Algae Tran, Miller; Henry, Ryan E.; Siefker, David Biotechnology and Bioengineering, Vol. 110, Issue 11 https://doi.org/10.1002/bit.24966	journal	June 2013
Hydrogen Fuel Production by Transgenic Microalgae Melis, Anastasios; Seibert, Michael; Ghirardi, Maria L. Transgenic Microalgae as Green Cell Factories https://doi.org/10.1007/978-0-387-75532-8_10	book	January 2007
Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes Li, Ting; Huang, Sheng; Zhao, Xuefeng Nucleic Acids Research, Vol. 39, Issue 14 https://doi.org/10.1093/nar/gkr188	journal	March 2011
TALE activation of endogenous genes in Chlamydomonas reinhardtii Gao, Han; Wright, David A.; Li, Ting Algal Research, Vol. 5 https://doi.org/10.1016/j.algal.2014.05.003	journal	July 2014
Improvement of hydrogen yield of lba-transgenic Chlamydomonas reinhardtii caused by increasing respiration and impairing photosynthesis Xu, Lili; Wang, Quanxi; Wu, Shuangxiu International Journal of Hydrogen Energy, Vol. 39, Issue 25 https://doi.org/10.1016/j.ijhydene.2014.04.178	journal	August 2014
Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis Cordero, Baldo F.; Couso, Inmaculada; León, Rosa Applied Microbiology and Biotechnology, Vol. 91, Issue 2 https://doi.org/10.1007/s00253-011-3262-y	journal	April 2011
An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance Schmollinger, Stefan; Strenkert, Daniela; Schroda, Michael Current Genetics, Vol. 56, Issue 4 https://doi.org/10.1007/s00294-010-0304-4	journal	May 2010
Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast Manuell, Andrea L.; Beligni, Maria Verónica; Elder, John H. Plant Biotechnology Journal, Vol. 5, Issue 3 https://doi.org/10.1111/j.1467-7652.2007.00249.x	journal	May 2007
An updated comprehensive techno-economic analysis of algae biodiesel Nagarajan, Sanjay; Chou, Siaw Kiang; Cao, Shenyan Bioresource Technology, Vol. 145 https://doi.org/10.1016/j.biortech.2012.11.108	journal	October 2013
Artificial control of transgene expression in Chlamydomonas reinhardtii chloroplast using the lac regulation system from Escherichia coli Kato, Ko; Marui, Takahiro; Kasai, Seitaro Journal of Bioscience and Bioengineering, Vol. 104, Issue 3 https://doi.org/10.1263/jbb.104.207	journal	September 2007
Importance of algae oil as a source of biodiesel Demirbas, Ayhan; Fatih Demirbas, M. Energy Conversion and Management, Vol. 52, Issue 1 https://doi.org/10.1016/j.enconman.2010.06.055	journal	January 2011
Photobioreactors for mass cultivation of algae Ugwu, C. U.; Aoyagi, H.; Uchiyama, H. Bioresource Technology, Vol. 99, Issue 10, p. 4021-4028 https://doi.org/10.1016/j.biortech.2007.01.046	journal	July 2008
Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains Work, Victoria H.; Radakovits, Randor; Jinkerson, Robert E. Eukaryotic Cell, Vol. 9, Issue 8 https://doi.org/10.1128/EC.00075-10	journal	June 2010
Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks Yoon, So-Mi; Kim, So Young; Li, Kun Feng Applied Microbiology and Biotechnology, Vol. 91, Issue 3 https://doi.org/10.1007/s00253-011-3279-2	journal	April 2011
Algal biodiversity Norton, Trevor A.; Melkonian, Michael; Andersen, Robert A. Phycologia, Vol. 35, Issue 4 https://doi.org/10.2216/i0031-8884-35-4-308.1	journal	July 1996
Recombination and Heterologous Expression of Allophycocyanin Gene in the Chloroplast of Chlamydomonas reinhardtii Su, Zhong-Liang; Qian, Kai-Xian; Tan, Cong-Ping Acta Biochimica et Biophysica Sinica, Vol. 37, Issue 10 https://doi.org/10.1111/j.1745-7270.2005.00092.x	journal	October 2005
Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses Rasala, Beth A.; Mayfield, Stephen P. Photosynthesis Research, Vol. 123, Issue 3 https://doi.org/10.1007/s11120-014-9994-7	journal	March 2014
System and method for research-scale outdoor production of microalgae and cyanobacteria Schoepp, Nathan G.; Stewart, Ryan L.; Sun, Vincent Bioresource Technology, Vol. 166 https://doi.org/10.1016/j.biortech.2014.05.046	journal	August 2014
The future viability of algae-derived biodiesel under economic and technical uncertainties Brownbridge, George; Azadi, Pooya; Smallbone, Andrew Bioresource Technology, Vol. 151 https://doi.org/10.1016/j.biortech.2013.10.062	journal	January 2014
TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation Merchant, Sabeeha S.; Kropat, Janette; Liu, Bensheng Current Opinion in Biotechnology, Vol. 23, Issue 3 https://doi.org/10.1016/j.copbio.2011.12.001	journal	June 2012
Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169 Msanne, Joseph; Xu, Di; Konda, Anji Reddy Phytochemistry, Vol. 75 https://doi.org/10.1016/j.phytochem.2011.12.007	journal	March 2012
Increased hydrogen photoproduction by means of a sulfur-deprived Chlamydomonas reinhardtii D1 protein mutant Torzillo, Giuseppe; Scoma, Alberto; Faraloni, Cecilia International Journal of Hydrogen Energy, Vol. 34, Issue 10 https://doi.org/10.1016/j.ijhydene.2008.07.093	journal	May 2009
Biopharmaceutical benchmarks 2014 Walsh, Gary Nature Biotechnology, Vol. 32, Issue 10 https://doi.org/10.1038/nbt.3040	journal	October 2014
The Roles of acyl-CoA: Diacylglycerol Acyltransferase 2 Genes in the Biosynthesis of Triacylglycerols by the Green Algae Chlamydomonas reinhardtii Deng, Xiao-Dong; Gu, Bo; Li, Ya-Jun Molecular Plant, Vol. 5, Issue 4 https://doi.org/10.1093/mp/sss040	journal	July 2012
Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology Näätsaari, Laura; Mistlberger, Beate; Ruth, Claudia PLoS ONE, Vol. 7, Issue 6 https://doi.org/10.1371/journal.pone.0039720	journal	June 2012
The Conserved TFLK Motif of Mammary-Associated Serum Amyloid A3 Is Responsible for Up-regulation of Intestinal MUC3 Mucin Expression In Vitro Mack, David R.; McDonald, Thomas L.; Larson, Marilynn A. Pediatric Research, Vol. 53, Issue 1 https://doi.org/10.1203/00006450-200301000-00023	journal	January 2003
RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii Moellering, Eric R.; Benning, Christoph Eukaryotic Cell, Vol. 9, Issue 1 https://doi.org/10.1128/EC.00203-09	journal	November 2009
Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection Dreesen, Imke A. J.; Hamri, Ghislaine Charpin-El; Fussenegger, Martin Journal of Biotechnology, Vol. 145, Issue 3 https://doi.org/10.1016/j.jbiotec.2009.12.006	journal	February 2010
Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii: Artificial miRNAs in Chlamydomonas Molnar, Attila; Bassett, Andrew; Thuenemann, Eva The Plant Journal, Vol. 58, Issue 1 https://doi.org/10.1111/j.1365-313X.2008.03767.x	journal	January 2009
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice Jiang, Wenzhi; Zhou, Huanbin; Bi, Honghao Nucleic Acids Research, Vol. 41, Issue 20 https://doi.org/10.1093/nar/gkt780	journal	August 2013
Biohydrogen production using mutant strains of Chlamydomonas reinhardtii: The effects of light intensity and illumination patterns Oncel, S. S.; Kose, A.; Faraloni, C. Biochemical Engineering Journal, Vol. 92 https://doi.org/10.1016/j.bej.2014.06.019	journal	November 2014
Production of unique immunotoxin cancer therapeutics in algal chloroplasts Tran, M.; Van, C.; Barrera, D. J. Proceedings of the National Academy of Sciences, Vol. 110, Issue 1 https://doi.org/10.1073/pnas.1214638110	journal	December 2012
The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii Goodenough, Ursula; Blaby, Ian; Casero, David Eukaryotic Cell, Vol. 13, Issue 5 https://doi.org/10.1128/EC.00013-14	journal	February 2014
Edible oils from microalgae: insights in TAG accumulation Klok, A. J.; Lamers, P. P.; Martens, D. E. Trends in Biotechnology, Vol. 32, Issue 10 https://doi.org/10.1016/j.tibtech.2014.07.004	journal	October 2014
Synthetic Oligonucleotide Libraries Reveal Novel Regulatory Elements in Chlamydomonas Chloroplast mRNAs Specht, Elizabeth A.; Mayfield, Stephen P. ACS Synthetic Biology, Vol. 2, Issue 1 https://doi.org/10.1021/sb300069k	journal	September 2012
Outdoor cultivation of microalgae for carotenoid production: current state and perspectives Del Campo, José A.; García-González, Mercedes; Guerrero, Miguel G. Applied Microbiology and Biotechnology, Vol. 74, Issue 6 https://doi.org/10.1007/s00253-007-0844-9	journal	February 2007
Production of recombinant enzymes in the marine alga Dunaliella tertiolecta Georgianna, D. Ryan; Hannon, Michael J.; Marcuschi, Marina Algal Research, Vol. 2, Issue 1 https://doi.org/10.1016/j.algal.2012.10.004	journal	January 2013
Manipulating Fatty Acid Biosynthesis in Microalgae for Biofuel through Protein-Protein Interactions Blatti, Jillian L.; Beld, Joris; Behnke, Craig A. PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0042949	journal	September 2012
Strategies to facilitate transgene expression in Chlamydomonas reinhardtii Eichler-Stahlberg, Alke; Weisheit, Wolfram; Ruecker, Ovidiu Planta, Vol. 229, Issue 4 https://doi.org/10.1007/s00425-008-0879-x	journal	January 2009
Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas Boyle, Nanette R.; Page, Mark Dudley; Liu, Bensheng Journal of Biological Chemistry, Vol. 287, Issue 19 https://doi.org/10.1074/jbc.M111.334052	journal	March 2012
Strategies for psbA gene expression in cyanobacteria, green algae and higher plants: From transcription to PSII repair Mulo, Paula; Sakurai, Isamu; Aro, Eva-Mari Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 1 https://doi.org/10.1016/j.bbabio.2011.04.011	journal	January 2012
Decoding algal genomes: tracing back the history of photosynthetic life on Earth: Decoding algal genomes Tirichine, Leïla; Bowler, Chris The Plant Journal, Vol. 66, Issue 1 https://doi.org/10.1111/j.1365-313X.2011.04540.x	journal	March 2011
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels Dismukes, G. Charles; Carrieri, Damian; Bennette, Nicholas Current Opinion in Biotechnology, Vol. 19, Issue 3 https://doi.org/10.1016/j.copbio.2008.05.007	journal	June 2008
Mechanism and challenges in commercialisation of algal biofuels Singh, Anoop; Nigam, Poonam Singh; Murphy, Jerry D. Bioresource Technology, Vol. 102, Issue 1 https://doi.org/10.1016/j.biortech.2010.06.057	journal	January 2011
High-throughput fluorescence-activated cell sorting for lipid hyperaccumulating Chlamydomonas reinhardtii mutants Xie, Bo; Stessman, Dan; Hart, Jason H. Plant Biotechnology Journal, Vol. 12, Issue 7 https://doi.org/10.1111/pbi.12190	journal	April 2014
Genetic Engineering to Improve Algal Biofuels Production Rasala, Beth A.; Gimpel, Javier A.; Tran, Miller Algae for Biofuels and Energy https://doi.org/10.1007/978-94-007-5479-9_6	book	November 2012
Outdoor H2 production in a 50-L tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii Scoma, Alberto; Giannelli, Luca; Faraloni, Cecilia Journal of Biotechnology, Vol. 157, Issue 4 https://doi.org/10.1016/j.jbiotec.2011.06.040	journal	February 2012
Studies on homologous recombination in the green alga Chlamydomonas reinhardtii Gumpel, Nicola J.; Rochaix, Jean-David; Purton, Saul Current Genetics, Vol. 26, Issue 5-6 https://doi.org/10.1007/BF00309931	journal	January 1994
Identification and microbial production of a terpene-based advanced biofuel Peralta-Yahya, Pamela P.; Ouellet, Mario; Chan, Rossana Nature Communications, Vol. 2, Issue 1 https://doi.org/10.1038/ncomms1494	journal	September 2011
Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves Siaut, Magali; Cuiné, Stéphan; Cagnon, Caroline BMC Biotechnology, Vol. 11, Issue 1 https://doi.org/10.1186/1472-6750-11-7	journal	January 2011
Plastidial Expression of Type II NAD(P)H Dehydrogenase Increases the Reducing State of Plastoquinones and Hydrogen Photoproduction Rate by the Indirect Pathway in Chlamydomonas reinhardtii Baltz, Anthony; Dang, Kieu-Van; Beyly, Audrey Plant Physiology, Vol. 165, Issue 3 https://doi.org/10.1104/pp.114.240432	journal	May 2014
Efficient H2 production via Chlamydomonas reinhardtii Esquível, Maria G.; Amaro, Helena M.; Pinto, Teresa S. Trends in Biotechnology, Vol. 29, Issue 12 https://doi.org/10.1016/j.tibtech.2011.06.008	journal	December 2011
Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii Jiang, Wenzhi; Brueggeman, Andrew J.; Horken, Kempton M. Eukaryotic Cell, Vol. 13, Issue 11 https://doi.org/10.1128/EC.00213-14	journal	September 2014
Structural Correlates of Cytoplasmic and Chloroplast Lipid Body Synthesis in Chlamydomonas reinhardtii and Stimulation of Lipid Body Production with Acetate Boost Goodson, Carrie; Roth, Robyn; Wang, Zi Teng Eukaryotic Cell, Vol. 10, Issue 12 https://doi.org/10.1128/EC.05242-11	journal	October 2011
Selenoprotein-Transgenic Chlamydomonas reinhardtii Hou, Qintang; Qiu, Shi; Liu, Qiong Nutrients, Vol. 5, Issue 3 https://doi.org/10.3390/nu5030624	journal	February 2013
Exploiting diversity and synthetic biology for the production of algal biofuels Georgianna, D. Ryan; Mayfield, Stephen P. Nature, Vol. 488, Issue 7411 https://doi.org/10.1038/nature11479	journal	August 2012
Lipid productivity as a key characteristic for choosing algal species for biodiesel production Griffiths, Melinda J.; Harrison, Susan T. L. Journal of Applied Phycology, Vol. 21, Issue 5 https://doi.org/10.1007/s10811-008-9392-7	journal	January 2009
Targeted disruption of the NIT8 gene in Chlamydomonas reinhardtii. Nelson, J. A.; Lefebvre, P. A. Molecular and Cellular Biology, Vol. 15, Issue 10 https://doi.org/10.1128/MCB.15.10.5762	journal	October 1995
Genomic Insights into Marine Microalgae Parker, Micaela S.; Mock, Thomas; Armbrust, E. Virginia Annual Review of Genetics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev.genet.42.110807.091417	journal	December 2008
Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms Ghirardi, Maria L.; Posewitz, Matthew C.; Maness, Pin-Ching Annual Review of Plant Biology, Vol. 58, Issue 1 https://doi.org/10.1146/annurev.arplant.58.032806.103848	journal	June 2007
Assembly of the Light-Harvesting Chlorophyll Antenna in the Green Alga Chlamydomonas reinhardtii Requires Expression of the TLA2 - CpFTSY Gene Kirst, Henning; García-Cerdán, Jose Gines; Zurbriggen, Andreas Plant Physiology, Vol. 158, Issue 2 https://doi.org/10.1104/pp.111.189910	journal	November 2011
The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics Rasala, Beth A.; Mayfield, Stephen P. Bioengineered Bugs, Vol. 2, Issue 1 https://doi.org/10.4161/bbug.2.1.13423	journal	January 2011
Gene silencing by artificial microRNAs in Chlamydomonas: Gene silencing by artificial miRNAs in Chlamydomonas Zhao, Tao; Wang, Wei; Bai, Xue The Plant Journal, Vol. 58, Issue 1 https://doi.org/10.1111/j.1365-313X.2008.03758.x	journal	December 2008
Survival of Human Metallothionein-2 Transplastomic Chlamydomonas reinhardtii to Ultraviolet B Exposure Zhang, Yu-Kun; Shen, Gui-Fang; Ru, Bing-Gen Acta Biochimica et Biophysica Sinica, Vol. 38, Issue 3 https://doi.org/10.1111/j.1745-7270.2006.00148.x	journal	March 2006
Engineered Photosystem II Reaction Centers Optimize Photochemistry versus Photoprotection at Different Solar Intensities Vinyard, David J.; Gimpel, Javier; Ananyev, Gennady M. Journal of the American Chemical Society, Vol. 136, Issue 10 https://doi.org/10.1021/ja5002967	journal	February 2014
Natural Variants of Photosystem II Subunit D1 Tune Photochemical Fitness to Solar Intensity Vinyard, David J.; Gimpel, Javier; Ananyev, Gennady M. Journal of Biological Chemistry, Vol. 288, Issue 8 https://doi.org/10.1074/jbc.M112.394668	journal	December 2012
Stable Integration and Functional Expression of Flounder Growth Hormone Gene in Transformed Microalga, Chlorella ellipsoidea Kim, Dae-Hyun; Kim, Young Tae; Cho, Jung Jong Marine Biotechnology, Vol. 4, Issue 1 https://doi.org/10.1007/s1012601-0070-x	journal	January 2002
Human serum amyloid A3 peptide enhances intestinal MUC3 expression and inhibits EPEC adherence Larson, Marilynn A.; Wei, Shu H.; Weber, Annika Biochemical and Biophysical Research Communications, Vol. 300, Issue 2 https://doi.org/10.1016/S0006-291X(02)02901-7	journal	January 2003
Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast Yang, Zongqi; Li, yinü; Chen, Feng Chinese Science Bulletin, Vol. 51, Issue 14 https://doi.org/10.1007/s11434-006-2041-0	journal	July 2006
Algal Lipid Bodies: Stress Induction, Purification, and Biochemical Characterization in Wild-Type and Starchless Chlamydomonas reinhardtii Wang, Zi Teng; Ullrich, Nico; Joo, Sunjoo Eukaryotic Cell, Vol. 8, Issue 12 https://doi.org/10.1128/EC.00272-09	journal	October 2009
Unravelling the genomic mosaic of a ubiquitous genus of marine cyanobacteria Dufresne, Alexis; Ostrowski, Martin; Scanlan, David J. Genome Biology, Vol. 9, Issue 5 https://doi.org/10.1186/gb-2008-9-5-r90	journal	January 2008
Chloroplast transformation in Chlamydomonas with high velocity microprojectiles Boynton, J.; Gillham, N.; Harris, E. Science, Vol. 240, Issue 4858 https://doi.org/10.1126/science.2897716	journal	June 1988
Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives Rosales-Mendoza, Sergio; Paz-Maldonado, Luz María Teresita; Soria-Guerra, Ruth Elena Plant Cell Reports, Vol. 31, Issue 3 https://doi.org/10.1007/s00299-011-1186-8	journal	November 2011
Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii Rasala, Beth A.; Barrera, Daniel J.; Ng, Jenny The Plant Journal, Vol. 74, Issue 4 https://doi.org/10.1111/tpj.12165	journal	April 2013
A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants Terashima, Mia; Freeman, Elizabeth S.; Jinkerson, Robert E. The Plant Journal, Vol. 81, Issue 1 https://doi.org/10.1111/tpj.12682	journal	October 2014
Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas. Cerutti, H.; Johnson, A. M.; Gillham, N. W. The Plant Cell, Vol. 9, Issue 6 https://doi.org/10.1105/tpc.9.6.925	journal	June 1997
Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide Rasala, Beth A.; Lee, Philip A.; Shen, Zhouxin PLoS ONE, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pone.0043349	journal	August 2012
Production of recombinant proteins in microalgae at pilot greenhouse scale: Recombinant Protein Production in Algae Gimpel, Javier A.; Hyun, James S.; Schoepp, Nathan G. Biotechnology and Bioengineering, Vol. 112, Issue 2 https://doi.org/10.1002/bit.25357	journal	September 2014

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