Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications

Young, Eric J.; Burton, Rodney; Mahalik, Jyoti P.; Sumpter, Bobby G.; Fuentes-Cabrera, Miguel; Kerfeld, Cheryl A.; Ducat, Daniel C.

doi:10.3389/fmicb.2017.01441

Title: Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications

Journal Article · Mon Jul 31 00:00:00 EDT 2017 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2017.01441· OSTI ID:1399948

Young, Eric J. ^[1]; Burton, Rodney ^[1]; Mahalik, Jyoti P. ^[2]; Sumpter, Bobby G. ^[2]; Fuentes-Cabrera, Miguel ^[2]; Kerfeld, Cheryl A. ^[3]; Ducat, Daniel C. ^[1]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Computational Sciences and Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division

As synthetic biology advances the intricacy of engineered biological systems, the importance of spatial organization within the cellular environment must not be marginalized. Increasingly, biological engineers are investigating means to control spatial organization within the cell, mimicking strategies used by natural pathways to increase flux and reduce cross-talk. A modular platform for constructing a diverse set of defined, programmable architectures would greatly assist in improving yields from introduced metabolic pathways and increasing insulation of other heterologous systems. Here, we review recent research on the shell proteins of bacterial microcompartments and discuss their potential application as “building blocks” for a range of customized intracellular scaffolds. As a result, we summarize the state of knowledge on the self-assembly of BMC shell proteins and discuss future avenues of research that will be important to realize the potential of BMC shell proteins as predictively assembling and programmable biological materials for bioengineering.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725; FG02-91ER20021

OSTI ID:: 1399948

Alternate ID(s):: OSTI ID: 1687363

Journal Information:: Frontiers in Microbiology, Vol. 8; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 35 works

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References (79)

Bacterial microcompartments and the modular construction of microbial metabolism Kerfeld, Cheryl A.; Erbilgin, Onur Trends in Microbiology, Vol. 23, Issue 1 https://doi.org/10.1016/j.tim.2014.10.003	journal	January 2015
The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene: Function of the PduJ microcompartment shell protein Chowdhury, Chiranjit; Chun, Sunny; Sawaya, Michael R. Molecular Microbiology, Vol. 101, Issue 5 https://doi.org/10.1111/mmi.13423	journal	June 2016
Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli Liang, Mingzhi; Frank, Stefanie; Lünsdorf, Heinrich Biotechnology Journal, Vol. 12, Issue 3 https://doi.org/10.1002/biot.201600415	journal	February 2017
Principles of assembly reveal a periodic table of protein complexes Ahnert, S. E.; Marsh, J. A.; Hernandez, H. Science, Vol. 350, Issue 6266 https://doi.org/10.1126/science.aaa2245	journal	December 2015
β-Carboxysomal proteins assemble into highly organized structures in Nicotiana chloroplasts Lin, Myat T.; Occhialini, Alessandro; Andralojc, P. John The Plant Journal, Vol. 79, Issue 1 https://doi.org/10.1111/tpj.12536	journal	June 2014
Bacterial Microcompartments Bobik, Thomas A. Microbe Magazine, Vol. 2, Issue 1 https://doi.org/10.1128/microbe.2.25.1	journal	January 2007
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A. PLoS Computational Biology, Vol. 10, Issue 10 https://doi.org/10.1371/journal.pcbi.1003898	journal	October 2014
Many-molecule encapsulation by an icosahedral shell Perlmutter, Jason D.; Mohajerani, Farzaneh; Hagan, Michael F. eLife, Vol. 5 https://doi.org/10.7554/eLife.14078	journal	May 2016
Natural strategies for the spatial optimization of metabolism in synthetic biology Agapakis, Christina M.; Boyle, Patrick M.; Silver, Pamela A. Nature Chemical Biology, Vol. 8, Issue 6 https://doi.org/10.1038/nchembio.975	journal	May 2012
Engineering Bacterial Microcompartment Shells: Chimeric Shell Proteins and Chimeric Carboxysome Shells Cai, Fei; Sutter, Markus; Bernstein, Susan L. ACS Synthetic Biology, Vol. 4, Issue 4 https://doi.org/10.1021/sb500226j	journal	July 2014
Two-dimensional crystals of carboxysome shell proteins recapitulate the hexagonal packing of three-dimensional crystals Dryden, Kelly A.; Crowley, Christopher S.; Tanaka, Shiho Protein Science, Vol. 18, Issue 12 https://doi.org/10.1002/pro.272	journal	December 2009
Enzyme clustering accelerates processing of intermediates through metabolic channeling Castellana, Michele; Wilson, Maxwell Z.; Xu, Yifan Nature Biotechnology, Vol. 32, Issue 10 https://doi.org/10.1038/nbt.3018	journal	September 2014
Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures Fu, Jinglin; Liu, Minghui; Liu, Yan Journal of the American Chemical Society, Vol. 134, Issue 12 https://doi.org/10.1021/ja300897h	journal	March 2012
Spatially Ordered Dynamics of the Bacterial Carbon Fixation Machinery Savage, D. F.; Afonso, B.; Chen, A. H. Science, Vol. 327, Issue 5970 https://doi.org/10.1126/science.1186090	journal	March 2010
Modularity of a carbon-fixing protein organelle Bonacci, W.; Teng, P. K.; Afonso, B. Proceedings of the National Academy of Sciences, Vol. 109, Issue 2 https://doi.org/10.1073/pnas.1108557109	journal	December 2011
Heterologous expression of the Halothiobacillus neapolitanus carboxysomal gene cluster in Corynebacterium glutamicum Baumgart, Meike; Huber, Isabel; Abdollahzadeh, Iman Journal of Biotechnology, Vol. 258 https://doi.org/10.1016/j.jbiotec.2017.03.019	journal	September 2017
Channeling by Proximity: The Catalytic Advantages of Active Site Colocalization Using Brownian Dynamics Bauler, Patricia; Huber, Gary; Leyh, Thomas The Journal of Physical Chemistry Letters, Vol. 1, Issue 9 https://doi.org/10.1021/jz1002007	journal	April 2010
Bacterial microcompartment assembly: The key role of encapsulation peptides Aussignargues, Clément; Paasch, Bradley C.; Gonzalez-Esquer, Raul Communicative & Integrative Biology, Vol. 8, Issue 3 https://doi.org/10.1080/19420889.2015.1039755	journal	May 2015
Challenges and opportunities for structural DNA nanotechnology Pinheiro, Andre V.; Han, Dongran; Shih, William M. Nature Nanotechnology, Vol. 6, Issue 12 https://doi.org/10.1038/nnano.2011.187	journal	November 2011
Streamlined Construction of the Cyanobacterial CO ₂ -Fixing Organelle via Protein Domain Fusions for Use in Plant Synthetic Biology Gonzalez-Esquer, C. Raul; Shubitowski, Tyler B.; Kerfeld, Cheryl A. The Plant Cell, Vol. 27, Issue 9 https://doi.org/10.1105/tpc.15.00329	journal	August 2015
Synthetic Protein Scaffolds Based on Peptide Motifs and Cognate Adaptor Domains for Improving Metabolic Productivity Horn, Anselm H. C.; Sticht, Heinrich Frontiers in Bioengineering and Biotechnology, Vol. 3 https://doi.org/10.3389/fbioe.2015.00191	journal	November 2015
Bacterial Microcompartment Organelles: Protein Shell Structure and Evolution Yeates, Todd O.; Crowley, Christopher S.; Tanaka, Shiho Annual Review of Biophysics, Vol. 39, Issue 1 https://doi.org/10.1146/annurev.biophys.093008.131418	journal	April 2010
Engineered Protein Nano-Compartments for Targeted Enzyme Localization Choudhary, Swati; Quin, Maureen B.; Sanders, Mark A. PLoS ONE, Vol. 7, Issue 3 https://doi.org/10.1371/journal.pone.0033342	journal	March 2012
Spatial organization of enzymes for metabolic engineering Lee, Hanson; DeLoache, William C.; Dueber, John E. Metabolic Engineering, Vol. 14, Issue 3 https://doi.org/10.1016/j.ymben.2011.09.003	journal	May 2012
Engineering formation of multiple recombinant Eut protein nanocompartments in E. coli Held, Mark; Kolb, Alexander; Perdue, Sarah Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep24359	journal	April 2016
Theoretical Study of the Initial Stages of Self-Assembly of a Carboxysome’s Facet Mahalik, J. P.; Brown, Kirsten A.; Cheng, Xiaolin ACS Nano, Vol. 10, Issue 6 https://doi.org/10.1021/acsnano.5b07805	journal	March 2016
Encapsulation of multiple cargo proteins within recombinant Eut nanocompartments Quin, Maureen B.; Perdue, Sarah A.; Hsu, Szu-Yi Applied Microbiology and Biotechnology, Vol. 100, Issue 21 https://doi.org/10.1007/s00253-016-7737-8	journal	July 2016
The Structure of CcmP, a Tandem Bacterial Microcompartment Domain Protein from the β-Carboxysome, Forms a Subcompartment Within a Microcompartment Cai, Fei; Sutter, Markus; Cameron, Jeffrey C. Journal of Biological Chemistry, Vol. 288, Issue 22 https://doi.org/10.1074/jbc.M113.456897	journal	April 2013
DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency Conrado, Robert J.; Wu, Gabriel C.; Boock, Jason T. Nucleic Acids Research, Vol. 40, Issue 4 https://doi.org/10.1093/nar/gkr888	journal	October 2011
Protein Assembly: Versatile Approaches to Construct Highly Ordered Nanostructures Luo, Quan; Hou, Chunxi; Bai, Yushi Chemical Reviews, Vol. 116, Issue 22 https://doi.org/10.1021/acs.chemrev.6b00228	journal	September 2016
Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli Lee, Matthew J.; Brown, Ian R.; Juodeikis, Rokas Metabolic Engineering, Vol. 36 https://doi.org/10.1016/j.ymben.2016.02.007	journal	July 2016
Identification and Structural Analysis of a Novel Carboxysome Shell Protein with Implications for Metabolite Transport Klein, Michael G.; Zwart, Peter; Bagby, Sarah C. Journal of Molecular Biology, Vol. 392, Issue 2 https://doi.org/10.1016/j.jmb.2009.03.056	journal	September 2009
Undesired usage and the robust self-assembly of heterogeneous structures Murugan, Arvind; Zou, James; Brenner, Michael P. Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms7203	journal	February 2015
Protein Structures Forming the Shell of Primitive Bacterial Organelles Kerfeld, C. A. Science, Vol. 309, Issue 5736 https://doi.org/10.1126/science.1113397	journal	August 2005
Encapsulation as a Strategy for the Design of Biological Compartmentalization Giessen, Tobias W.; Silver, Pamela A. Journal of Molecular Biology, Vol. 428, Issue 5 https://doi.org/10.1016/j.jmb.2015.09.009	journal	February 2016
Engineering bacterial microcompartments with heterologous enzyme cargos Wagner, Hanna J.; Capitain, Charlotte C.; Richter, Karsten Engineering in Life Sciences, Vol. 17, Issue 1 https://doi.org/10.1002/elsc.201600107	journal	September 2016
Engineering enzymatic cascades on nanoscale scaffolds Idan, Ofer; Hess, Henry Current Opinion in Biotechnology, Vol. 24, Issue 4 https://doi.org/10.1016/j.copbio.2013.01.003	journal	August 2013
Light Modulates the Biosynthesis and Organization of Cyanobacterial Carbon Fixation Machinery through Photosynthetic Electron Flow Sun, Yaqi; Casella, Selene; Fang, Yi Plant Physiology, Vol. 171, Issue 1 https://doi.org/10.1104/pp.16.00107	journal	March 2016
Interactions and structural variability of β-carboxysomal shell protein CcmL Keeling, Thomas J.; Samborska, Bożena; Demers, Ryan W. Photosynthesis Research, Vol. 121, Issue 2-3 https://doi.org/10.1007/s11120-014-9973-z	journal	February 2014
Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments Fan, C.; Cheng, S.; Sinha, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 37 https://doi.org/10.1073/pnas.1207516109	journal	August 2012
Principles of Protein−Protein Interactions: What are the Preferred Ways For Proteins To Interact? Keskin, Ozlem; Gursoy, Attila; Ma, Buyong Chemical Reviews, Vol. 108, Issue 4 https://doi.org/10.1021/cr040409x	journal	April 2008
Enzyme cascades activated on topologically programmed DNA scaffolds Wilner, Ofer I.; Weizmann, Yossi; Gill, Ron Nature Nanotechnology, Vol. 4, Issue 4 https://doi.org/10.1038/nnano.2009.50	journal	March 2009
Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy Conrado, Robert J.; Varner, Jeffrey D.; DeLisa, Matthew P. Current Opinion in Biotechnology, Vol. 19, Issue 5 https://doi.org/10.1016/j.copbio.2008.07.006	journal	October 2008
An iterative method for extracting energy-like quantities from protein structures. Thomas, P. D.; Dill, K. A. Proceedings of the National Academy of Sciences, Vol. 93, Issue 21 https://doi.org/10.1073/pnas.93.21.11628	journal	October 1996
Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli Yung, Mimi C.; Bourguet, Feliza A.; Carpenter, Timothy S. Microbial Cell Factories, Vol. 16, Issue 1 https://doi.org/10.1186/s12934-017-0685-x	journal	April 2017
Bacterial Microcompartments Kerfeld, Cheryl A.; Heinhorst, Sabine; Cannon, Gordon C. Annual Review of Microbiology, Vol. 64, Issue 1 https://doi.org/10.1146/annurev.micro.112408.134211	journal	October 2010
A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments Kim, Edward Y.; Tullman-Ercek, Danielle Biotechnology Journal, Vol. 9, Issue 3 https://doi.org/10.1002/biot.201300391	journal	January 2014
Localization of Proteins to the 1,2-Propanediol Utilization Microcompartment by Non-native Signal Sequences Is Mediated by a Common Hydrophobic Motif Jakobson, Christopher M.; Kim, Edward Y.; Slininger, Marilyn F. Journal of Biological Chemistry, Vol. 290, Issue 40 https://doi.org/10.1074/jbc.M115.651919	journal	August 2015
Structural Insights into Higher Order Assembly and Function of the Bacterial Microcompartment Protein PduA Pang, Allan; Frank, Stefanie; Brown, Ian Journal of Biological Chemistry, Vol. 289, Issue 32 https://doi.org/10.1074/jbc.M114.569285	journal	May 2014
Synthetic scaffolds for pathway enhancement Siu, Ka-Hei; Chen, Rebecca P.; Sun, Qing Current Opinion in Biotechnology, Vol. 36 https://doi.org/10.1016/j.copbio.2015.08.009	journal	December 2015
Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell Sutter, Markus; Greber, Basil; Aussignargues, Clement Science, Vol. 356, Issue 6344 https://doi.org/10.1126/science.aan3289	journal	June 2017
Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis Parsons, Joshua B.; Dinesh, Sriramulu D.; Deery, Evelyne Journal of Biological Chemistry, Vol. 283, Issue 21 https://doi.org/10.1074/jbc.M709214200	journal	March 2008
Visualization of Bacterial Microcompartment Facet Assembly Using High-Speed Atomic Force Microscopy Sutter, Markus; Faulkner, Matthew; Aussignargues, Clément Nano Letters, Vol. 16, Issue 3 https://doi.org/10.1021/acs.nanolett.5b04259	journal	December 2015
Atomic-Level Models of the Bacterial Carboxysome Shell Tanaka, S.; Kerfeld, C. A.; Sawaya, M. R. Science, Vol. 319, Issue 5866 https://doi.org/10.1126/science.1151458	journal	February 2008
Signaling Through Scaffold, Anchoring, and Adaptor Proteins Pawson, T. Science, Vol. 278, Issue 5346 https://doi.org/10.1126/science.278.5346.2075	journal	December 1997
Synthetic protein scaffolds provide modular control over metabolic flux Dueber, John E.; Wu, Gabriel C.; Malmirchegini, G. Reza Nature Biotechnology, Vol. 27, Issue 8, p. 753-759 https://doi.org/10.1038/nbt.1557	journal	August 2009
Genetic encoding of DNA nanostructures and their self-assembly in living bacteria Elbaz, Johann; Yin, Peng; Voigt, Christopher A. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms11179	journal	April 2016
Scaffold Proteins: Hubs for Controlling the Flow of Cellular Information Good, M. C.; Zalatan, J. G.; Lim, W. A. Science, Vol. 332, Issue 6030 https://doi.org/10.1126/science.1198701	journal	May 2011
PduA Is a Shell Protein of Polyhedral Organelles Involved in Coenzyme B12-Dependent Degradation of 1,2-Propanediol in Salmonella enterica Serovar Typhimurium LT2 Havemann, G. D.; Sampson, E. M.; Bobik, T. A. Journal of Bacteriology, Vol. 184, Issue 5 https://doi.org/10.1128/JB.184.5.1253-1261.2002	journal	March 2002
Protein Calligraphy: A New Concept Begins To Take Shape Glover, Dominic J.; Clark, Douglas S. ACS Central Science, Vol. 2, Issue 7 https://doi.org/10.1021/acscentsci.6b00067	journal	June 2016
A Dodecameric CcmK2 Structure Suggests β-Carboxysomal Shell Facets Have a Double-Layered Organization Samborska, Bożena; Kimber, Matthew S. Structure, Vol. 20, Issue 8 https://doi.org/10.1016/j.str.2012.05.013	journal	August 2012
Elucidating Essential Role of Conserved Carboxysomal Protein CcmN Reveals Common Feature of Bacterial Microcompartment Assembly Kinney, James N.; Salmeen, Annette; Cai, Fei Journal of Biological Chemistry, Vol. 287, Issue 21 https://doi.org/10.1074/jbc.M112.355305	journal	March 2012
Structural Insight into the Mechanisms of Transport across the Salmonella enterica Pdu Microcompartment Shell Crowley, Christopher S.; Cascio, Duilio; Sawaya, Michael R. Journal of Biological Chemistry, Vol. 285, Issue 48 https://doi.org/10.1074/jbc.M110.160580	journal	September 2010
Substrate channelling as an approach to cascade reactions Wheeldon, Ian; Minteer, Shelley D.; Banta, Scott Nature Chemistry, Vol. 8, Issue 4 https://doi.org/10.1038/nchem.2459	journal	March 2016
Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway Cameron, Jeffrey C.; Wilson, Steven C.; Bernstein, Susan L. Cell, Vol. 155, Issue 5 https://doi.org/10.1016/j.cell.2013.10.044	journal	November 2013
Placing molecules with Bohr radius resolution using DNA origami Funke, Jonas J.; Dietz, Hendrik Nature Nanotechnology, Vol. 11, Issue 1 https://doi.org/10.1038/nnano.2015.240	journal	October 2015
Using synthetic RNAs as scaffolds and regulators Myhrvold, Cameron; Silver, Pamela A. Nature Structural & Molecular Biology, Vol. 22, Issue 1 https://doi.org/10.1038/nsmb.2944	journal	January 2015
Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli Takenoya, M.; Nikolakakis, K.; Sagermann, M. Journal of Bacteriology, Vol. 192, Issue 22 https://doi.org/10.1128/JB.00652-10	journal	September 2010
Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor Lawrence, Andrew D.; Frank, Stefanie; Newnham, Sarah ACS Synthetic Biology, Vol. 3, Issue 7 https://doi.org/10.1021/sb4001118	journal	November 2013
Structural Insight into the Clostridium difficile Ethanolamine Utilisation Microcompartment Pitts, Alison C.; Tuck, Laura R.; Faulds-Pain, Alexandra PLoS ONE, Vol. 7, Issue 10 https://doi.org/10.1371/journal.pone.0048360	journal	October 2012
Alanine Scanning Mutagenesis Identifies an Asparagine–Arginine–Lysine Triad Essential to Assembly of the Shell of the Pdu Microcompartment Sinha, Sharmistha; Cheng, Shouqiang; Sung, Yea Won Journal of Molecular Biology, Vol. 426, Issue 12 https://doi.org/10.1016/j.jmb.2014.04.012	journal	June 2014
Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement Parsons, Joshua B.; Frank, Stefanie; Bhella, David Molecular Cell, Vol. 38, Issue 2 https://doi.org/10.1016/j.molcel.2010.04.008	journal	April 2010
Assembly of Robust Bacterial Microcompartment Shells Using Building Blocks from an Organelle of Unknown Function Lassila, Jonathan K.; Bernstein, Susan L.; Kinney, James N. Journal of Molecular Biology, Vol. 426, Issue 11 https://doi.org/10.1016/j.jmb.2014.02.025	journal	May 2014
Comparative analysis of carboxysome shell proteins Kinney, James N.; Axen, Seth D.; Kerfeld, Cheryl A. Photosynthesis Research, Vol. 109, Issue 1-3 https://doi.org/10.1007/s11120-011-9624-6	journal	January 2011
Insights from multiple structures of the shell proteins from the β-carboxysome Tanaka, Shiho; Sawaya, Michael R.; Phillips, Martin Protein Science https://doi.org/10.1002/pro.14	journal	January 2008
Rationally engineering natural protein assemblies in nanobiotechnology Howorka, Stefan Current Opinion in Biotechnology, Vol. 22, Issue 4 https://doi.org/10.1016/j.copbio.2011.05.003	journal	August 2011
Evidence for Improved Encapsulated Pathway Behavior in a Bacterial Microcompartment through Shell Protein Engineering Slininger Lee, Marilyn F.; Jakobson, Christopher M.; Tullman-Ercek, Danielle ACS Synthetic Biology, Vol. 6, Issue 10 https://doi.org/10.1021/acssynbio.7b00042	journal	June 2017
Rewiring Cells: Synthetic Biology as a Tool to Interrogate the Organizational Principles of Living Systems Bashor, Caleb J.; Horwitz, Andrew A.; Peisajovich, Sergio G. Annual Review of Biophysics, Vol. 39, Issue 1 https://doi.org/10.1146/annurev.biophys.050708.133652	journal	April 2010
Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri Heldt, Dana; Frank, Stefanie; Seyedarabi, Arefeh Biochemical Journal, Vol. 423, Issue 2 https://doi.org/10.1042/BJ20090780	journal	September 2009

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Protein Gradients on the Nucleoid Position the Carbon-Fixing Organelles of Cyanobacteria MacCready, Joshua S.; Hakim, Pusparanee; Young, Eric J. SSRN Electronic Journal https://doi.org/10.2139/ssrn.3192036	journal	January 2018
Bacterial Microcompartments Bobik, Thomas A. Microbe Magazine, Vol. 2, Issue 1 https://doi.org/10.1128/microbe.2.25.1	journal	January 2007
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Deciphering molecular details in the assembly of alpha-type carboxysome Liu, Yilan; He, Xinyuan; Lim, Weiping Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-33074-x	journal	October 2018
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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
scaffold
synthetic biology
bacterial microcompartment
shell proteins
BMC
spatial organization
metabolic engineering
self-assembly

Title: Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications

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References (79)

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