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Title: Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications

Abstract

As synthetic biology advances the intricacy of engineered biological systems, the importance of spatial organization within the cellular environment must not be marginalized. Increasingly, biological engineers are investigating means to control spatial organization within the cell, mimicking strategies used by natural pathways to increase flux and reduce cross-talk. A modular platform for constructing a diverse set of defined, programmable architectures would greatly assist in improving yields from introduced metabolic pathways and increasing insulation of other heterologous systems. Here, we review recent research on the shell proteins of bacterial microcompartments and discuss their potential application as “building blocks” for a range of customized intracellular scaffolds. As a result, we summarize the state of knowledge on the self-assembly of BMC shell proteins and discuss future avenues of research that will be important to realize the potential of BMC shell proteins as predictively assembling and programmable biological materials for bioengineering.

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [1];  [2];  [5];  [1];  [2]
  1. Michigan State Univ., East Lansing, MI (United States)
  2. (United States)
  3. MSU-DOE Plant Research Lab., East Lansing, MI (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  5. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1399948
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; scaffold; synthetic biology; bacterial microcompartment; shell proteins; BMC; spatial organization; metabolic engineering; self-assembly

Citation Formats

Young, Eric J., MSU-DOE Plant Research Lab., East Lansing, MI, Burton, Rodney, Mahalik, Jyoti P., Sumpter, Bobby G., Fuentes-Cabrera, Miguel A., Kerfeld, Cheryl A., MSU-DOE Plant Research Lab., East Lansing, MI, Lawrence Berkeley National Lab., Ducat, Daniel C., and MSU-DOE Plant Research Lab., East Lansing, MI. Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications. United States: N. p., 2017. Web. doi:10.3389/fmicb.2017.01441.
Young, Eric J., MSU-DOE Plant Research Lab., East Lansing, MI, Burton, Rodney, Mahalik, Jyoti P., Sumpter, Bobby G., Fuentes-Cabrera, Miguel A., Kerfeld, Cheryl A., MSU-DOE Plant Research Lab., East Lansing, MI, Lawrence Berkeley National Lab., Ducat, Daniel C., & MSU-DOE Plant Research Lab., East Lansing, MI. Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications. United States. doi:10.3389/fmicb.2017.01441.
Young, Eric J., MSU-DOE Plant Research Lab., East Lansing, MI, Burton, Rodney, Mahalik, Jyoti P., Sumpter, Bobby G., Fuentes-Cabrera, Miguel A., Kerfeld, Cheryl A., MSU-DOE Plant Research Lab., East Lansing, MI, Lawrence Berkeley National Lab., Ducat, Daniel C., and MSU-DOE Plant Research Lab., East Lansing, MI. Mon . "Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications". United States. doi:10.3389/fmicb.2017.01441. https://www.osti.gov/servlets/purl/1399948.
@article{osti_1399948,
title = {Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications},
author = {Young, Eric J. and MSU-DOE Plant Research Lab., East Lansing, MI and Burton, Rodney and Mahalik, Jyoti P. and Sumpter, Bobby G. and Fuentes-Cabrera, Miguel A. and Kerfeld, Cheryl A. and MSU-DOE Plant Research Lab., East Lansing, MI and Lawrence Berkeley National Lab. and Ducat, Daniel C. and MSU-DOE Plant Research Lab., East Lansing, MI},
abstractNote = {As synthetic biology advances the intricacy of engineered biological systems, the importance of spatial organization within the cellular environment must not be marginalized. Increasingly, biological engineers are investigating means to control spatial organization within the cell, mimicking strategies used by natural pathways to increase flux and reduce cross-talk. A modular platform for constructing a diverse set of defined, programmable architectures would greatly assist in improving yields from introduced metabolic pathways and increasing insulation of other heterologous systems. Here, we review recent research on the shell proteins of bacterial microcompartments and discuss their potential application as “building blocks” for a range of customized intracellular scaffolds. As a result, we summarize the state of knowledge on the self-assembly of BMC shell proteins and discuss future avenues of research that will be important to realize the potential of BMC shell proteins as predictively assembling and programmable biological materials for bioengineering.},
doi = {10.3389/fmicb.2017.01441},
journal = {Frontiers in Microbiology},
number = ,
volume = 8,
place = {United States},
year = {Mon Jul 31 00:00:00 EDT 2017},
month = {Mon Jul 31 00:00:00 EDT 2017}
}

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Works referenced in this record:

Synthetic protein scaffolds provide modular control over metabolic flux
journal, August 2009

  • Dueber, John E.; Wu, Gabriel C.; Malmirchegini, G. Reza
  • Nature Biotechnology, Vol. 27, Issue 8, p. 753-759
  • DOI: 10.1038/nbt.1557