A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Priye, Aashish; Bird, Sara W.; Light, Yooli K.; Ball, Cameron S.; Negrete, Oscar A.; Meagher, Robert J.

doi:10.1038/srep44778

A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Journal Article · Mon Mar 20 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/srep44778· OSTI ID:1399884

Priye, Aashish ^[1]; Bird, Sara W. ^[1]; Light, Yooli K. ^[2]; Ball, Cameron S. ^[1]; Negrete, Oscar A. ^[1]; Meagher, Robert J. ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biotechnology and Bioengineering
Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Systems Biology

Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. We demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. The smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Altogether, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1399884

Alternate ID(s):: OSTI ID: 1510601

Report Number(s):: SAND2016--11988J; srep44778

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 1 of Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform Ahn, Su; Baek, Yun; Lloren, Khristine https://doi.org/10.6084/m9.figshare.9210812.v1 The current record is supplemented by this text	text	January 2019
Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform Ahn, Su; Baek, Yun; Lloren, Khristine https://doi.org/10.6084/m9.figshare.c.4604882.v1 The current record is supplemented by this collection	collection	January 2019

A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

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