‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family

Kleindienst, Sara; Higgins, Steven A.; Tsementzi, Despina; Chen, Gao; Konstantinidis, Konstantinos T.; Mack, E. Erin; Löffler, Frank E.

doi:10.1016/j.syapm.2016.12.001

‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family

Journal Article · Wed Dec 21 00:00:00 EST 2016 · Systematic and Applied Microbiology

DOI:https://doi.org/10.1016/j.syapm.2016.12.001· OSTI ID:1399390

^[1]; Higgins, Steven A. ^[1]; Tsementzi, Despina ^[2]; Chen, Gao ^[3]; Konstantinidis, Konstantinos T. ^[4]; Mack, E. Erin ^[5]; ^[6]

Univ. of Tennessee and Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology
Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering, School of Biology
Corporate Remediation Group, Newark, DE (United States)
Univ. of Tennessee and Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering

Taxonomic assignments of anaerobic dichloromethane (DCM)-degrading bacteria remain poorly constrained but are important for understanding the microbial diversity of organisms contributing to DCM turnover in environmental systems. Here, we describe the taxonomic classification of a novel DCM degrader in consortium RM obtained from pristine Rio Mameyes sediment. Phylogenetic analysis of full-length 16S rRNA gene sequences demonstrated that the DCM degrader was most closely related to members of the genera Dehalobacter and Syntrophobotulus, but sequence similarities did not exceed 94% and 93%, respectively. Genome-aggregate average amino acid identities against Peptococcaceae members did not exceed 66%, suggesting that the DCM degrader does not affiliate with any described genus. Phylogenetic analysis of conserved single-copy functional genes supported that the DCM degrader represents a novel clade. Growth strictly depended on the presence of DCM, which was consumed at a rate of 160 ± 3 μmol L^-1 d^-1. The DCM degrader attained 5.25 × 10⁷ ± 1.0 × 10⁷ cells per μmol DCM consumed. Fluorescence in situ hybridization revealed rod-shaped cells 4 ± 0.8 μm long and 0.4 ± 0.1 μm wide. Furthermore, based on the unique phylogenetic, genomic, and physiological characteristics, we propose that the DCM degrader represents a new genus and species, ‘Candidatus Dichloromethanomonas elyunquensis’.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1399390

Journal Information:: Systematic and Applied Microbiology, Journal Name: Systematic and Applied Microbiology Journal Issue: 3 Vol. 40; ISSN 0723-2020

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family

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