RsaM: a transcriptional regulator of Burkholderia spp. with novel fold

Michalska, Karolina; Chhor, Gekleng; Clancy, Shonda; Jedrzejczak, Robert; Babnigg, Gyorgy; Winans, Stephen C.; Joachimiak, Andrzej

doi:10.1111/febs.12868

RsaM: a transcriptional regulator of Burkholderia spp. with novel fold

Journal Article · Fri Jul 04 04:00:00 EDT 2014 · Federation of European Biochemical Societies (FEBS) Journal

DOI:https://doi.org/10.1111/febs.12868· OSTI ID:1396289

Michalska, Karolina ^[1]; Chhor, Gekleng ^[2]; Clancy, Shonda ^[2]; Jedrzejczak, Robert ^[2]; Babnigg, Gyorgy ^[2]; Winans, Stephen C. ^[3]; Joachimiak, Andrzej ^[4]

Midwest Center for Structural Genomics, Argonne National Laboratory, IL USA; Structural Biology Center, Biosciences Division, Argonne National Laboratory, IL USA
Midwest Center for Structural Genomics, Argonne National Laboratory, IL USA
Department of Microbiology, Cornell University, Ithaca NY USA
Midwest Center for Structural Genomics, Argonne National Laboratory, IL USA; Structural Biology Center, Biosciences Division, Argonne National Laboratory, IL USA; Department of Biochemistry and Molecular Biology, University of Chicago, IL USA

Burkholderia cepacia complex (Bcc) is a set of closely related bacterial species that are notorious pathogens of cystic fibrosis patients, responsible for life-threatening lung infections. Expression of several virulence factors of Bcc is controlled by a mechanism known as quorum sensing (QS). QS is a means of bacterial communication used to coordinate gene expression in a cell-density-dependent manner. The system involves the production of diffusible signaling molecules (N-acyl-L-homoserine lactones, AHLs), that bind to cognate transcriptional regulators and influence their ability to regulate gene expression. One such system that is highly conserved in Bcc consists of CepI and CepR. CepI is AHL synthase, while CepR is an AHL-dependent transcription factor. In most members of the Bcc group, the cepI and cepR genes are divergently transcribed and separated by additional genes. One of them, bcam1869, encodes the BcRsaM protein, which was recently postulated to modulate the abundance or activity of CepI or CepR. Here we show the crystal structure of BcRsaM from B. cenocepacia J2315. It is a single-domain protein with unique topology and presents a novel fold. The protein is a dimer in the crystal and in solution. This regulator has no known DNA binding motifs and direct binding of BcRsaM to the cepI promoter could not be detected in in vitro assays. Therefore, we propose that the modulatory action of RsaM might result from interactions with other components of the QS machinery rather than from direct association with the DNA promoter.

Research Organization:: Argonne National Laboratory (ANL)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science - Office of Biological and Environmental Research

DOE Contract Number:: AC02-06CH11357

OSTI ID:: 1396289

Journal Information:: Federation of European Biochemical Societies (FEBS) Journal, Journal Name: Federation of European Biochemical Societies (FEBS) Journal Journal Issue: 18 Vol. 281; ISSN 1742-464X

Publisher:: Federation of European Biochemical Societies

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
BcRsaM
Burkholderia cenocepacia
TofM
bcam1869
quorum sensing

RsaM: a transcriptional regulator of Burkholderia spp. with novel fold

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