Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

Jaing, Crystal; Rowland, Raymond R.  R.; Allen, Jonathan E.; Certoma, Andrea; Thissen, James B.; Bingham, John; Rowe, Brenton; White, John R.; Wynne, James W.; Johnson, Dayna; Gaudreault, Natasha N.; Williams, David T.

doi:10.1038/s41598-017-10186-4

Title: Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

Journal Article · Thu Aug 31 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-10186-4· OSTI ID:1395491

Jaing, Crystal ^[1]; Rowland, Raymond R. R. ^[2]; Allen, Jonathan E. ^[3]; Certoma, Andrea ^[4]; Thissen, James B. ^[1]; Bingham, John ^[4]; Rowe, Brenton ^[4]; White, John R. ^[4]; Wynne, James W. ^[4]; Johnson, Dayna ^[4]; Gaudreault, Natasha N. ^[2]; Williams, David T. ^[4]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physical & Life Sciences Directorate
Kansas State Univ., Manhattan, KS (United States). Department of Diagnostic Medicine and Pathobiology
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Computation Directorate
CSIRO Australian Animal Health Laboratory, Geelong, Victoria (Australia)

African swine fever virus (ASFV) is a macrophage-tropic virus responsible for ASF, a transboundary disease that threatens swine production world-wide. Since there are no vaccines available to control ASF after an outbreak, obtaining an understanding of the virus-host interaction is important for developing new intervention strategies. In this study, a whole transcriptomic RNA-Seq method was used to characterize differentially expressed genes in pigs infected with a low pathogenic ASFV isolate, OUR T88/3 (OURT), or the highly pathogenic Georgia 2007/1 (GRG). After infection, pigs infected with OURT showed no or few clinical signs; whereas, GRG produced clinical signs consistent with acute ASF. RNA-Seq detected the expression of ASFV genes from the whole blood of the GRG, but not the OURT pigs, consistent with the pathotypes of these strains and the replication of GRG in circulating monocytes. Even though GRG and OURT possess different pathogenic properties, there was significant overlap in the most upregulated host genes. A small number of differentially expressed microRNAs were also detected in GRG and OURT pigs. These data confirm previous studies describing the response of macrophages and lymphocytes to ASFV infection, as well as reveal unique gene pathways upregulated in response to infection with GRG.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1395491

Journal Information:: Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 27 works

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Identification and Immunogenicity of African Swine Fever Virus Antigens Netherton, Christopher L.; Goatley, Lynnette C.; Reis, Ana Luisa Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.01318	journal	June 2019
African swine fever virus does not express viral microRNAs in experimentally infected pigs Núñez-Hernández, Fernando; Vera, Gonzalo; Sánchez, Armand BMC Veterinary Research, Vol. 14, Issue 1 https://doi.org/10.1186/s12917-018-1601-2	journal	September 2018
RNA-seq reveals downregulated osteochondral genes potentially related to tibia bacterial chondronecrosis with osteomyelitis in broilers de Oliveira, Haniel Cedraz; Ibelli, Adriana Mércia Guaratini; Guimarães, Simone Eliza Facioni BMC Genetics, Vol. 21, Issue 1 https://doi.org/10.1186/s12863-020-00862-2	journal	June 2020
Mechanisms of African swine fever virus pathogenesis and immune evasion inferred from gene expression changes in infected swine macrophages Zhu, James J.; Ramanathan, Palaniappan; Bishop, Elizabeth A. PLOS ONE, Vol. 14, Issue 11 https://doi.org/10.1371/journal.pone.0223955	journal	November 2019
Identification and characterization of the 285L and K145R proteins of African swine fever virus Hübner, Alexandra; Keßler, Catharina; Pannhorst, Katrin Journal of General Virology, Vol. 100, Issue 9 https://doi.org/10.1099/jgv.0.001306	journal	September 2019
The effects of a globin blocker on the resolution of 3’mRNA sequencing data in porcine blood Lim, Kyu-Sang; Dong, Qian; Moll, Pamela BMC Genomics, Vol. 20, Issue 1 https://doi.org/10.1186/s12864-019-6122-2	journal	October 2019

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