skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Variation in short-term and long-term responses of photosynthesis and isoprenoid-mediated photoprotection to soil water availability in four Douglas-fir provenances

Abstract

For long-lived forest tree species, the understanding of intraspecific variation among populations and their response to water availability can reveal their ability to cope with and adapt to climate change. Dissipation of excess excitation energy, mediated by photoprotective isoprenoids, is an important defense mechanism against drought and high light when photosynthesis is hampered. We used 50-year-old Douglas-fir trees of four provenances at two common garden experiments to characterize provenance-specific variation in photosynthesis and photoprotective mechanisms mediated by essential and non-essential isoprenoids in response to soil water availability and solar radiation. All provenances revealed uniform photoprotective responses to high solar radiation, including increased de-epoxidation of photoprotective xanthophyll cycle pigments and enhanced emission of volatile monoterpenes. In contrast, we observed differences between provenances in response to drought, where provenances sustaining higher CO2 assimilation rates also revealed increased water-use efficiency, carotenoid-chlorophyll ratios, pools of xanthophyll cycle pigments, β-carotene and stored monoterpenes. Our results demonstrate that local adaptation to contrasting habitats affected chlorophyll-carotenoid ratios, pool sizes of photoprotective xanthophylls, β-carotene, and stored volatile isoprenoids. We conclude that intraspecific variation in isoprenoid-mediated photoprotective mechanisms contributes to the adaptive potential of Douglas-fir provenances to climate change.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [8];  [2];  [9];  [10]
  1. Univ. of Toronto, ON (Canada). Department of Biology, Graduate Programs in Cell & Systems Biology and Ecology & Evolutionary Biology; Forstliche Versuchs- und Forschungsanstalt Baden-Wurttemberg (Germany); Forschungszentrum Julich GmbH (Germany). Institute of Bio and Geosciences IBG-2, Plant Sciences
  2. Albert-Ludwigs University Freiburg (Germany). Chair of Tree Physiology, Institute of Forest Sciences
  3. Institute for Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research (ZALF), Muncheberg (Germany); Leuphana University of Luneburg (Germany). Institute of Ecology
  4. Forstliche Versuchs- und Forschungsanstalt Baden-Wurttemberg (Germany); HAWK University of Applied Sciences and Arts (Germany). Hildesheim/Holzminden/Gottingen, Faculty of Resource Management
  5. Univ. of Toronto, ON (Canada). Department of Biology, Graduate Programs in Cell & Systems Biology and Ecology & Evolutionary Biology; Forstliche Versuchs- und Forschungsanstalt Baden-Wurttemberg (Germany); Albert-Ludwigs University, Freiburg (Germany). Institute of Biology III, Faculty of Biology
  6. Institute for Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research (ZALF), Muncheberg (Germany); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). USDA Forest Service, Northern Research Station
  7. Albert-Ludwigs-University, Freiburg (Germany). Centre for Biosystems Analysis (ZBSA)
  8. Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum Munchen, Neuherberg (Germany)
  9. Institute for Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research (ZALF), Muncheberg (Germany); Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB) (Germany); Swiss Federal Research Institute WSL, Birmensdorf (Switzerland)
  10. Univ. of Toronto, ON (Canada). Department of Biology, Graduate Programs in Cell & Systems Biology and Ecology & Evolutionary Biology; Forstliche Versuchs- und Forschungsanstalt Baden-Wurttemberg (Germany)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1395485
Grant/Contract Number:  
AC52-07NA27344
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; Drought; Light responses; Natural variation in plants; Non-photochemical quenching; Plant physiology

Citation Formats

Junker, Laura Verena, Kleiber, Anita, Jansen, Kirstin, Wildhagen, Henning, Hess, Moritz, Kayler, Zachary, Kammerer, Bernd, Schnitzler, Jörg-Peter, Kreuzwieser, Jürgen, Gessler, Arthur, and Ensminger, Ingo. Variation in short-term and long-term responses of photosynthesis and isoprenoid-mediated photoprotection to soil water availability in four Douglas-fir provenances. United States: N. p., 2017. Web. doi:10.1038/srep40145.
Junker, Laura Verena, Kleiber, Anita, Jansen, Kirstin, Wildhagen, Henning, Hess, Moritz, Kayler, Zachary, Kammerer, Bernd, Schnitzler, Jörg-Peter, Kreuzwieser, Jürgen, Gessler, Arthur, & Ensminger, Ingo. Variation in short-term and long-term responses of photosynthesis and isoprenoid-mediated photoprotection to soil water availability in four Douglas-fir provenances. United States. doi:10.1038/srep40145.
Junker, Laura Verena, Kleiber, Anita, Jansen, Kirstin, Wildhagen, Henning, Hess, Moritz, Kayler, Zachary, Kammerer, Bernd, Schnitzler, Jörg-Peter, Kreuzwieser, Jürgen, Gessler, Arthur, and Ensminger, Ingo. Tue . "Variation in short-term and long-term responses of photosynthesis and isoprenoid-mediated photoprotection to soil water availability in four Douglas-fir provenances". United States. doi:10.1038/srep40145. https://www.osti.gov/servlets/purl/1395485.
@article{osti_1395485,
title = {Variation in short-term and long-term responses of photosynthesis and isoprenoid-mediated photoprotection to soil water availability in four Douglas-fir provenances},
author = {Junker, Laura Verena and Kleiber, Anita and Jansen, Kirstin and Wildhagen, Henning and Hess, Moritz and Kayler, Zachary and Kammerer, Bernd and Schnitzler, Jörg-Peter and Kreuzwieser, Jürgen and Gessler, Arthur and Ensminger, Ingo},
abstractNote = {For long-lived forest tree species, the understanding of intraspecific variation among populations and their response to water availability can reveal their ability to cope with and adapt to climate change. Dissipation of excess excitation energy, mediated by photoprotective isoprenoids, is an important defense mechanism against drought and high light when photosynthesis is hampered. We used 50-year-old Douglas-fir trees of four provenances at two common garden experiments to characterize provenance-specific variation in photosynthesis and photoprotective mechanisms mediated by essential and non-essential isoprenoids in response to soil water availability and solar radiation. All provenances revealed uniform photoprotective responses to high solar radiation, including increased de-epoxidation of photoprotective xanthophyll cycle pigments and enhanced emission of volatile monoterpenes. In contrast, we observed differences between provenances in response to drought, where provenances sustaining higher CO2 assimilation rates also revealed increased water-use efficiency, carotenoid-chlorophyll ratios, pools of xanthophyll cycle pigments, β-carotene and stored monoterpenes. Our results demonstrate that local adaptation to contrasting habitats affected chlorophyll-carotenoid ratios, pool sizes of photoprotective xanthophylls, β-carotene, and stored volatile isoprenoids. We conclude that intraspecific variation in isoprenoid-mediated photoprotective mechanisms contributes to the adaptive potential of Douglas-fir provenances to climate change.},
doi = {10.1038/srep40145},
journal = {Scientific Reports},
issn = {2045-2322},
number = ,
volume = 7,
place = {United States},
year = {2017},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 1 work
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Nonenzymic carotenoid oxidation and photooxidative stress signalling in plants
journal, August 2012

  • Ramel, Fanny; Mialoundama, Alexis S.; Havaux, Michel
  • Journal of Experimental Botany, Vol. 64, Issue 3
  • DOI: 10.1093/jxb/ers223

Drought effects on allocation of recent carbon: from beech leaves to soil CO2 efflux
journal, October 2009


Monoterpene emission from coniferous trees in response to elevated CO2 concentration and climate warming
journal, March 1999


Opportunistic emissions of volatile isoprenoids
journal, September 2005


Isoprene emission protects photosynthesis but reduces plant productivity during drought in transgenic tobacco ( Nicotiana tabacum ) plants
journal, September 2013

  • Ryan, Annette C.; Hewitt, C. Nicholas; Possell, Malcolm
  • New Phytologist, Vol. 201, Issue 1
  • DOI: 10.1111/nph.12477

Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants
journal, October 1998

  • Pogson, B. J.; Niyogi, K. K.; Bjorkman, O.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 22
  • DOI: 10.1073/pnas.95.22.13324

Abiotic stresses and induced BVOCs
journal, March 2010


Impact of drought on productivity and water use efficiency in 29 genotypes of Populus deltoides x Populus nigra
journal, February 2006


Conifer species adapt to low-rainfall climates by following one of two divergent pathways
journal, September 2014

  • Brodribb, Timothy J.; McAdam, Scott A. M.; Jordan, Gregory J.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 40
  • DOI: 10.1073/pnas.1407930111

Isoprenoids: an evolutionary pool for photoprotection
journal, April 2005


Photosynthetic performance and water relations in young pubescent oak ( Quercus pubescens ) trees during drought stress and recovery
journal, June 2007


Risk-taking plants: Anisohydric behavior as a stress-resistance trait
journal, July 2012

  • Sade, Nir; Gebremedhin, Alem; Moshelion, Menachem
  • Plant Signaling & Behavior, Vol. 7, Issue 7
  • DOI: 10.4161/psb.20505

How Plants Cope with Water Stress in the Field? Photosynthesis and Growth
journal, June 2002


Molecular genetics of xanthophyll–dependent photoprotection in green algae and plants
journal, October 2000

  • Baroli, Irene; Niyogi, Krishna K.
  • Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 355, Issue 1402
  • DOI: 10.1098/rstb.2000.0700

Photoprotection in an ecological context: the remarkable complexity of thermal energy dissipation
journal, October 2006


Carotenoid oxidation products as stress signals in plants
journal, December 2013


Chlorophyll fluorescence—a practical guide
journal, April 2000


Sap Pressure in Vascular Plants: Negative hydrostatic pressure can be measured in plants
journal, April 1965


Relationship between leaf optical properties, chlorophyll fluorescence and pigment changes in senescing Acer saccharum leaves
journal, February 2016


Natural strategies for photosynthetic light harvesting
journal, June 2014

  • Croce, Roberta; van Amerongen, Herbert
  • Nature Chemical Biology, Vol. 10, Issue 7
  • DOI: 10.1038/nchembio.1555

Mechanisms of plant survival and mortality during drought: why do some plants survive while others succumb to drought?
journal, June 2008


May photoinhibition be a consequence, rather than a cause, of limited plant productivity?
journal, May 2013

  • Adams, William W.; Muller, Onno; Cohu, Christopher M.
  • Photosynthesis Research, Vol. 117, Issue 1-3
  • DOI: 10.1007/s11120-013-9849-7

Intermittent low temperatures constrain spring recovery of photosynthesis in boreal Scots pine forests
journal, June 2004


Clear link between drought stress, photosynthesis and biogenic volatile organic compounds in Fagus sylvatica L.
journal, September 2011


Shoot and root vulnerability to xylem cavitation in four populations of Douglas-fir seedlings
journal, January 1999


Too much light? How β-carotene protects the photosystem II reaction centre
journal, January 2005

  • Telfer, Alison
  • Photochemical & Photobiological Sciences, Vol. 4, Issue 12
  • DOI: 10.1039/b507888c

A unified mechanism of action for volatile isoprenoids in plant abiotic stress
journal, April 2009

  • Vickers, Claudia E.; Gershenzon, Jonathan; Lerdau, Manuel T.
  • Nature Chemical Biology, Vol. 5, Issue 5
  • DOI: 10.1038/nchembio.158

Physiological and structural changes in response to altered precipitation regimes in a Mediterranean macchia ecosystem
journal, March 2009


Monoterpene levels in needles of Douglas fir exposed to elevated CO2 and temperature
journal, March 2003


Volatile isoprenoid emission potentials are correlated with essential isoprenoid concentrations in five plant species
journal, July 2013


Interactions of drought and shade effects on seedlings of four Quercus species: physiological and structural leaf responses
journal, June 2006


Adaptation, migration or extirpation: climate change outcomes for tree populations: Climate change outcomes for tree populations
journal, January 2008


Carbon Isotope Discrimination and Photosynthesis
journal, June 1989

  • Farquhar, G. D.; Ehleringer, J. R.; Hubick, K. T.
  • Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 40, Issue 1, p. 503-537
  • DOI: 10.1146/annurev.pp.40.060189.002443

Safety valves for photosynthesis
journal, December 2000


Carbon isotope discrimination by plants follows latitudinal and altitudinal trends
journal, September 1991

  • Körner, Ch.; Farquhar, G. D.; Wong, S. C.
  • Oecologia, Vol. 88, Issue 1
  • DOI: 10.1007/BF00328400