Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction

Journal Article · · Applied and Environmental Microbiology
DOI:https://doi.org/10.1128/AEM.00043-15· OSTI ID:1394408
 [1];  [2];  [1];  [1];  [1];  [3];  [3];  [3];  [3];  [3];  [2];  [3];  [4];  [4];  [5];  [6]
  1. Univ. of Oklahoma, Norman, OK (United States)
  2. Stanford Univ., CA (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Michigan State Univ., East Lansing, MI (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. Univ. of Oklahoma, Norman, OK (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Tsinghua Univ., Beijing (China)
+ Show Author Affiliations
A pilot-scale field experiment demonstrated that a one-time amendment of emulsified vegetable oil (EVO) reduced groundwater U(VI) concentrations for 1 year in a fast-flowing aquifer. However, little is known about how EVO amendment stimulates the functional gene composition, structure, and dynamics of groundwater microbial communities toward prolonged U(VI) reduction. In this paper, we hypothesized that EVO amendment would shift the functional gene composition and structure of groundwater microbial communities and stimulate key functional genes/groups involved in EVO biodegradation and reduction of electron acceptors in the aquifer. To test these hypotheses, groundwater microbial communities after EVO amendment were analyzed using a comprehensive functional gene microarray. Our results showed that EVO amendment stimulated sequential shifts in the functional composition and structure of groundwater microbial communities. Particularly, the relative abundance of key functional genes/groups involved in EVO biodegradation and the reduction of NO3-, Mn(IV), Fe(III), U(VI), and SO42- significantly increased, especially during the active U(VI) reduction period. The relative abundance for some of these key functional genes/groups remained elevated over 9 months. Montel tests suggested that the dynamics in the abundance, composition, and structure of these key functional genes/groups were significantly correlated with groundwater concentrations of acetate, NO3-, Mn(II), Fe(II), U(VI), and SO42-. Our results suggest that EVO amendment stimulated dynamic succession of key functional microbial communities. Finally, this study improves our understanding of the composition, structure, and function changes needed for groundwater microbial communities to sustain a long-term U(VI) reduction.
Research Organization:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Michigan State Univ., East Lansing, MI (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Oklahoma, Norman, OK (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
AC02-05CH11231; AC05-00OR22725; FG02-07ER64398
OSTI ID:
1394408
Alternate ID(s):
OSTI ID: 1511425
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 12 Vol. 81; ISSN 0099-2240
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

References (52)

Environmental proteomics reveals early microbial community responses to biostimulation at a uranium- and nitrate-contaminated site journal August 2013
Reduction of U(VI) to U(IV) by indigenous bacteria in contaminated ground water journal December 1998
Concurrent bioremediation of perchlorate and 1,1,1-trichloroethane in an emulsified oil barrier journal October 2007
XPS and XANES Studies of Uranium Reduction by Clostridium sp journal April 1994
In Situ Bioreduction of Technetium and Uranium in a Nitrate-Contaminated Aquifer journal January 2004
Pilot-Scale in Situ Bioremediation of Uranium in a Highly Contaminated Aquifer. 1. Conditioning of a Treatment Zone journal June 2006
Pilot-Scale in Situ Bioremedation of Uranium in a Highly Contaminated Aquifer. 2. Reduction of U(VI) and Geochemical Control of U(VI) Bioavailability journal June 2006
In Situ Bioreduction of Uranium (VI) to Submicromolar Levels and Reoxidation by Dissolved Oxygen journal August 2007
Effects of Nitrate on the Stability of Uranium in a Bioreduced Region of the Subsurface journal July 2010
In Situ Bioremediation of Uranium with Emulsified Vegetable Oil as the Electron Donor journal May 2013
U(VI) Bioreduction with Emulsified Vegetable Oil as the Electron Donor – Model Application to a Field Test journal March 2013
In Situ Long-Term Reductive Bioimmobilization of Cr(VI) in Groundwater Using Hydrogen Release Compound journal November 2008
Microbial reduction of uranium journal April 1991
Subsurface clade of Geobacteraceae that predominates in a diversity of Fe(III)-reducing subsurface environments journal October 2007
Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths journal September 2008
Responses of microbial community functional structures to pilot-scale uranium in situ bioremediation journal March 2010
GeoChip 3.0 as a high-throughput tool for analyzing microbial community composition, structure and functional activity journal April 2010
Biostimulation induces syntrophic interactions that impact C, S and N cycling in a sediment microbial community journal November 2012
Evaluation of Slow Release Substrates for Anaerobic Bioremediation journal July 2006
PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR journal July 1996
Comparison of 16S rRNA, nifD, recA, gyrB, rpoB and fusA genes within the family Geobacteraceae fam. nov. journal September 2004
Metagenomic analysis reveals a marked divergence in the structure of belowground microbial communities at elevated CO2: Changes in the soil microbial community at elevated CO2 journal April 2010
GeoChip-based analysis of functional microbial communities during the reoxidation of a bioreduced uranium-contaminated aquifer journal October 2009
Sulfate-reducing bacterium grows with Cr(VI), U(VI), Mn(IV), and Fe(III) as electron acceptors journal May 1998
A Limited Microbial Consortium Is Responsible for Extended Bioreduction of Uranium in a Contaminated Aquifer journal July 2011
Reduction of Uranium(VI) to Uranium(IV) by Clostridia journal May 2008
Molecular Analysis of the Metabolic Rates of Discrete Subsurface Populations of Sulfate Reducers journal July 2011
Significant Association between Sulfate-Reducing Bacteria and Uranium-Reducing Microbial Communities as Revealed by a Combined Massively Parallel Sequencing-Indicator Species Approach journal August 2010
Dynamics of Microbial Community Composition and Function during In Situ Bioremediation of a Uranium-Contaminated Aquifer journal April 2011
Microbial Communities in Contaminated Sediments, Associated with Bioremediation of Uranium to Submicromolar Levels journal May 2008
Diversity and Distribution of Anaeromyxobacter Strains in a Uranium-Contaminated Subsurface Environment with a Nonuniform Groundwater Flow journal April 2009
Microarray-Based Analysis of Subnanogram Quantities of Microbial Community DNAs by Using Whole-Community Genome Amplification journal July 2006
Functional Diversity and Electron Donor Dependence of Microbial Populations Capable of U(VI) Reduction in Radionuclide-Contaminated Subsurface Sediments journal March 2008
Microbial Functional Gene Diversity with a Shift of Subsurface Redox Conditions during In Situ Uranium Reduction journal February 2012
Reduction of uranium by Desulfovibrio desulfuricans. journal January 1992
Reduction of uranium by cytochrome c3 of Desulfovibrio vulgaris. journal January 1993
DNA recovery from soils of diverse composition. journal January 1996
Diversity and Characterization of Sulfate-Reducing Bacteria in Groundwater at a Uranium Mill Tailings Site journal July 2001
Uranium Reduction by Desulfovibrio desulfuricans Strain G20 and a Cytochrome c3 Mutant journal June 2002
Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer journal October 2003
Molecular Diversity of Sulfate-Reducing Bacteria from Two Different Continental Margin Habitats journal October 2003
Periplasmic Cytochrome c3 of Desulfovibrio vulgaris Is Directly Involved in H2-Mediated Metal but Not Sulfate Reduction journal January 2004
Change in Bacterial Community Structure during In Situ Biostimulation of Subsurface Sediment Cocontaminated with Uranium and Nitrate journal August 2004
Alkaline Anaerobic Respiration: Isolation and Characterization of a Novel Alkaliphilic and Metal-Reducing Bacterium journal September 2004
Diversity and Distribution of Sulfate-Reducing Bacteria in Permanently Frozen Lake Fryxell, McMurdo Dry Valleys, Antarctica journal October 2005
Phylogeography of Sulfate-Reducing Bacteria among Disturbed Sediments, Disclosed by Analysis of the Dissimilatory Sulfite Reductase Genes (dsrAB) journal February 2005
Uranium(VI) Reduction by Anaeromyxobacter dehalogenans Strain 2CP-C journal May 2006
Reduction of uranium by Desulfovibrio desulfuricans. journal January 1992
Reduction of uranium by cytochrome c3 of Desulfovibrio vulgaris. journal January 1993
DNA recovery from soils of diverse composition. journal January 1996
Uranium Reduction journal October 2006
Importance of c-Type cytochromes for U(VI) reduction by Geobacter sulfurreducens journal January 2007

Cited By (5)

Contrasting Ecological Processes and Functional Compositions Between Intestinal Bacterial Community in Healthy and Diseased Shrimp journal August 2016
Metagenomic applications in environmental monitoring and bioremediation journal August 2016
Network succession reveals the importance of competition in response to emulsified vegetable oil amendment for uranium bioremediation: Competition in bioremediation system journal August 2015
Microbial Functional Gene Diversity Predicts Groundwater Contamination and Ecosystem Functioning journal February 2018
Functional Gene Array-Based Ultrasensitive and Quantitative Detection of Microbial Populations in Complex Communities journal June 2019

Similar Records

A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction
Journal Article · Thu Apr 02 00:00:00 EDT 2015 · Environmental Science and Technology · OSTI ID:1470905
A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction
Journal Article · Wed Apr 01 20:00:00 EDT 2015 · Environmental Science and Technology · OSTI ID:1379163

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES